Evaluation of antibacterial properties of portland cement in combination with chitosan against sfrepfoccocus mutans

Caries is one of the most prevalent oral problems among people around the world. The main pathogenic bacteria that cause the problem is known as Streptococcus mutans. Tooth caries can be painful as it can cause pulp inflammation. Vital pulp therapies which include pulp capping and pulpotomy are the...

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Main Author:	Sudirman, Nur Hafizah
Format:	Thesis
Language:	English
Published:	2020
Subjects:	R Medicine
Online Access:	http://eprints.usm.my/47993/1/30.%20Thesis_Final%20Copy_THESIS_NUR%20NAJIHAH%20BINTI%20SIDEK_P-SKM0042_19-24%20pages.pdf
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spelling	my-usm-ep.479932020-12-15T03:07:49Z Evaluation of antibacterial properties of portland cement in combination with chitosan against sfrepfoccocus mutans 2020-08 Sudirman, Nur Hafizah R Medicine Caries is one of the most prevalent oral problems among people around the world. The main pathogenic bacteria that cause the problem is known as Streptococcus mutans. Tooth caries can be painful as it can cause pulp inflammation. Vital pulp therapies which include pulp capping and pulpotomy are the most commonly used treatment nowadays. Biomaterials that are commonly utilised in the treatment in clinics include mineral trioxide aggregate (MTA) and calcium hydroxide (Ca(OH)2); however these materials have some drawbacks. In this study, a new material known as Portland cement was synthesized with different concentrations of chitosan and antibacterial properties against S. mutan was investigated. Materials was prepared as follows: APC, APC-0.6%CHT, APC-1.25%CHT and APC-2.5%CHT. The direct contact test demonstrated a significant reduction in the growth curve in all treated groups compared to the control group. Similar findings were obtained in the biofilm formation test with APC-2.5%CHT showed the highest inhibition against S. mutans. Bacterial distribution analysis by scanning electron microscopy on APC-2.5%CHT showed that after 7 days of treatment, the number of distributed bacteria was decreased. Gene expression by RT-PCR demonstrated APC-2.5%CHT has significantly down-regulated gtfB and gbpB genes compared to APC alone. In conclusion, APC with different concentrations of chitosan exhibited antibacterial effects with APC-2.5%CHT demonstrated the highest inhibition against S. mutans. 2020-08 Thesis http://eprints.usm.my/47993/ http://eprints.usm.my/47993/1/30.%20Thesis_Final%20Copy_THESIS_NUR%20NAJIHAH%20BINTI%20SIDEK_P-SKM0042_19-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine
spellingShingle	R Medicine Sudirman, Nur Hafizah Evaluation of antibacterial properties of portland cement in combination with chitosan against sfrepfoccocus mutans
description	Caries is one of the most prevalent oral problems among people around the world. The main pathogenic bacteria that cause the problem is known as Streptococcus mutans. Tooth caries can be painful as it can cause pulp inflammation. Vital pulp therapies which include pulp capping and pulpotomy are the most commonly used treatment nowadays. Biomaterials that are commonly utilised in the treatment in clinics include mineral trioxide aggregate (MTA) and calcium hydroxide (Ca(OH)2); however these materials have some drawbacks. In this study, a new material known as Portland cement was synthesized with different concentrations of chitosan and antibacterial properties against S. mutan was investigated. Materials was prepared as follows: APC, APC-0.6%CHT, APC-1.25%CHT and APC-2.5%CHT. The direct contact test demonstrated a significant reduction in the growth curve in all treated groups compared to the control group. Similar findings were obtained in the biofilm formation test with APC-2.5%CHT showed the highest inhibition against S. mutans. Bacterial distribution analysis by scanning electron microscopy on APC-2.5%CHT showed that after 7 days of treatment, the number of distributed bacteria was decreased. Gene expression by RT-PCR demonstrated APC-2.5%CHT has significantly down-regulated gtfB and gbpB genes compared to APC alone. In conclusion, APC with different concentrations of chitosan exhibited antibacterial effects with APC-2.5%CHT demonstrated the highest inhibition against S. mutans.
format	Thesis
qualification_level	Master's degree
author	Sudirman, Nur Hafizah
author_facet	Sudirman, Nur Hafizah
author_sort	Sudirman, Nur Hafizah
title	Evaluation of antibacterial properties of portland cement in combination with chitosan against sfrepfoccocus mutans
title_short	Evaluation of antibacterial properties of portland cement in combination with chitosan against sfrepfoccocus mutans
title_full	Evaluation of antibacterial properties of portland cement in combination with chitosan against sfrepfoccocus mutans
title_fullStr	Evaluation of antibacterial properties of portland cement in combination with chitosan against sfrepfoccocus mutans
title_full_unstemmed	Evaluation of antibacterial properties of portland cement in combination with chitosan against sfrepfoccocus mutans
title_sort	evaluation of antibacterial properties of portland cement in combination with chitosan against sfrepfoccocus mutans
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kesihatan
publishDate	2020
url	http://eprints.usm.my/47993/1/30.%20Thesis_Final%20Copy_THESIS_NUR%20NAJIHAH%20BINTI%20SIDEK_P-SKM0042_19-24%20pages.pdf
_version_	1747821861002018816

Evaluation of antibacterial properties of portland cement in combination with chitosan against sfrepfoccocus mutans

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