Role of oxidative stress in rem sleep deprivation-induced endothelial dysfunction in rat model

Sleep deprivation is associated with endothelial dysfunction, an early sign of cardiovascular disease. The pathogenesis underlying endothelial dysfunction in sleep deprivation remains poorly understood to date. The objective of this study was to identify the possible factors involved in the mecha...

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Main Author:	Nor, Nur Syafiqah Mohmed
Format:	Thesis
Language:	English
Published:	2020
Subjects:	R Medicine
Online Access:	http://eprints.usm.my/47994/1/31.%20THESIS_NUR%20SYAFIQAH%20BINTI%20MOHMED%20NOR_P-SKM0062_19-24%20pages.pdf
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spelling	my-usm-ep.479942020-12-15T04:27:20Z Role of oxidative stress in rem sleep deprivation-induced endothelial dysfunction in rat model 2020-08 Nor, Nur Syafiqah Mohmed R Medicine Sleep deprivation is associated with endothelial dysfunction, an early sign of cardiovascular disease. The pathogenesis underlying endothelial dysfunction in sleep deprivation remains poorly understood to date. The objective of this study was to identify the possible factors involved in the mechanism of endothelial dysfunction in sleep deprivation, in particular REM sleep phase. Only experiments for the first objective were carried out in this study as a result of the Movement Control Order (MCO) of the Federal Government of Malaysia due to the pandemic of coronavirus disease (COVID-19). Eight (8) male Sprague-Dawley rats were equally divided into four groups; free-moving control rats (FMC), 72-h REM sleep-deprived rats (REMsd), tank control rats (TC) and sleep recovery for 72 hours after 72 hours of REM sleep deprivation rats (SR). Rats were deprived of REM sleep using the inverted flowerpot technique. There was no significant differences in the levels of food consumption, body weight gain and systolic blood pressure between the groups. The levels of protein expression of endothelial nitric oxide synthase (eNOS) and phosphorylated eNOS (peNOS), and the levels of oxidative stress markers in the aorta; total antioxidant capacity (TAC), catalase (CAT), and superoxide dismutase (SOD) were not measured due to time constraints. Based on the assumption that REM sleep deprivation induced endothelial dysfunction, it is expected that there will be a significant decrease in the levels of p-eNOS in the REMsd group compared to other groups. Oxidative stress has been postulated as a possible factor in the mechanism of endothelial dysfunction, thus a significant decrease in the levels of antioxidants is expected to occur in aortic tissue. In conclusion, it is postulated that REM sleep deprivation is associated with endothelial dysfunction that may be induced by oxidative stress 2020-08 Thesis http://eprints.usm.my/47994/ http://eprints.usm.my/47994/1/31.%20THESIS_NUR%20SYAFIQAH%20BINTI%20MOHMED%20NOR_P-SKM0062_19-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine
spellingShingle	R Medicine Nor, Nur Syafiqah Mohmed Role of oxidative stress in rem sleep deprivation-induced endothelial dysfunction in rat model
description	Sleep deprivation is associated with endothelial dysfunction, an early sign of cardiovascular disease. The pathogenesis underlying endothelial dysfunction in sleep deprivation remains poorly understood to date. The objective of this study was to identify the possible factors involved in the mechanism of endothelial dysfunction in sleep deprivation, in particular REM sleep phase. Only experiments for the first objective were carried out in this study as a result of the Movement Control Order (MCO) of the Federal Government of Malaysia due to the pandemic of coronavirus disease (COVID-19). Eight (8) male Sprague-Dawley rats were equally divided into four groups; free-moving control rats (FMC), 72-h REM sleep-deprived rats (REMsd), tank control rats (TC) and sleep recovery for 72 hours after 72 hours of REM sleep deprivation rats (SR). Rats were deprived of REM sleep using the inverted flowerpot technique. There was no significant differences in the levels of food consumption, body weight gain and systolic blood pressure between the groups. The levels of protein expression of endothelial nitric oxide synthase (eNOS) and phosphorylated eNOS (peNOS), and the levels of oxidative stress markers in the aorta; total antioxidant capacity (TAC), catalase (CAT), and superoxide dismutase (SOD) were not measured due to time constraints. Based on the assumption that REM sleep deprivation induced endothelial dysfunction, it is expected that there will be a significant decrease in the levels of p-eNOS in the REMsd group compared to other groups. Oxidative stress has been postulated as a possible factor in the mechanism of endothelial dysfunction, thus a significant decrease in the levels of antioxidants is expected to occur in aortic tissue. In conclusion, it is postulated that REM sleep deprivation is associated with endothelial dysfunction that may be induced by oxidative stress
format	Thesis
qualification_level	Master's degree
author	Nor, Nur Syafiqah Mohmed
author_facet	Nor, Nur Syafiqah Mohmed
author_sort	Nor, Nur Syafiqah Mohmed
title	Role of oxidative stress in rem sleep deprivation-induced endothelial dysfunction in rat model
title_short	Role of oxidative stress in rem sleep deprivation-induced endothelial dysfunction in rat model
title_full	Role of oxidative stress in rem sleep deprivation-induced endothelial dysfunction in rat model
title_fullStr	Role of oxidative stress in rem sleep deprivation-induced endothelial dysfunction in rat model
title_full_unstemmed	Role of oxidative stress in rem sleep deprivation-induced endothelial dysfunction in rat model
title_sort	role of oxidative stress in rem sleep deprivation-induced endothelial dysfunction in rat model
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2020
url	http://eprints.usm.my/47994/1/31.%20THESIS_NUR%20SYAFIQAH%20BINTI%20MOHMED%20NOR_P-SKM0062_19-24%20pages.pdf
_version_	1747821861251579904

Role of oxidative stress in rem sleep deprivation-induced endothelial dysfunction in rat model

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