Metastatic inhibitory effect of gallic acid combined with cisplatin on hela cells proliferation

Due to the multiple adverse effects of chemotherapy treatment, more researches with natural product discovery has been conducted to treat various types of cancer. A polyphenol derivative, gallic acid, was previously reported to combat the growth of cancer cells. Hence, this study aims to assess t...

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Main Author:	Zamberi, Nurul Nadia Mohamad
Format:	Thesis
Language:	English
Published:	2020
Subjects:	R Medicine
Online Access:	http://eprints.usm.my/47996/1/33.%20Thesis_Final%20Copy_THESIS_NURUL%20NADIA%20BINTI%20MOHAMAD%20ZAMBERI_P-SKM0035_19-24%20pages.pdf
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spelling	my-usm-ep.479962020-12-15T07:21:40Z Metastatic inhibitory effect of gallic acid combined with cisplatin on hela cells proliferation 2020 Zamberi, Nurul Nadia Mohamad R Medicine Due to the multiple adverse effects of chemotherapy treatment, more researches with natural product discovery has been conducted to treat various types of cancer. A polyphenol derivative, gallic acid, was previously reported to combat the growth of cancer cells. Hence, this study aims to assess the metastatic inhibitory effect of gallic acid combined with cisplatin against HeLa cells. Cell migration, cell invasion and cell proliferation were measured by scratch-wound healing assay, Transwell invasion assay and colony formation assay, respectively. After 24-hour of scratch-wound healing assay, the combined treatment showed significant decrease in wound closure rate where 43.08 ± 6.21% of the wound was filled by the HeLa cells in comparison to control wound width. Combination of gallic acid and cisplatin also exerted a significant decrease in the number of invaded cells at 44.55 ± 6.04% as compared to the control group by Transwell invasion assay. Gallic acid and cisplatin were able to significantly reduce the proliferation HeLa cells by colony formation assay. However, the effect that was produced by their combination was greater than that of each agent alone. Hence further study either in vitro and in vivo is required to strengthen the current findings such as research regarding the involvement of PI3K/AKT/mTOR signaling pathway in decreasing migration and invasion capability of HeLa cells. 2020 Thesis http://eprints.usm.my/47996/ http://eprints.usm.my/47996/1/33.%20Thesis_Final%20Copy_THESIS_NURUL%20NADIA%20BINTI%20MOHAMAD%20ZAMBERI_P-SKM0035_19-24%20pages.pdf application/pdf en public other Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine
spellingShingle	R Medicine Zamberi, Nurul Nadia Mohamad Metastatic inhibitory effect of gallic acid combined with cisplatin on hela cells proliferation
description	Due to the multiple adverse effects of chemotherapy treatment, more researches with natural product discovery has been conducted to treat various types of cancer. A polyphenol derivative, gallic acid, was previously reported to combat the growth of cancer cells. Hence, this study aims to assess the metastatic inhibitory effect of gallic acid combined with cisplatin against HeLa cells. Cell migration, cell invasion and cell proliferation were measured by scratch-wound healing assay, Transwell invasion assay and colony formation assay, respectively. After 24-hour of scratch-wound healing assay, the combined treatment showed significant decrease in wound closure rate where 43.08 ± 6.21% of the wound was filled by the HeLa cells in comparison to control wound width. Combination of gallic acid and cisplatin also exerted a significant decrease in the number of invaded cells at 44.55 ± 6.04% as compared to the control group by Transwell invasion assay. Gallic acid and cisplatin were able to significantly reduce the proliferation HeLa cells by colony formation assay. However, the effect that was produced by their combination was greater than that of each agent alone. Hence further study either in vitro and in vivo is required to strengthen the current findings such as research regarding the involvement of PI3K/AKT/mTOR signaling pathway in decreasing migration and invasion capability of HeLa cells.
format	Thesis
qualification_level	other
author	Zamberi, Nurul Nadia Mohamad
author_facet	Zamberi, Nurul Nadia Mohamad
author_sort	Zamberi, Nurul Nadia Mohamad
title	Metastatic inhibitory effect of gallic acid combined with cisplatin on hela cells proliferation
title_short	Metastatic inhibitory effect of gallic acid combined with cisplatin on hela cells proliferation
title_full	Metastatic inhibitory effect of gallic acid combined with cisplatin on hela cells proliferation
title_fullStr	Metastatic inhibitory effect of gallic acid combined with cisplatin on hela cells proliferation
title_full_unstemmed	Metastatic inhibitory effect of gallic acid combined with cisplatin on hela cells proliferation
title_sort	metastatic inhibitory effect of gallic acid combined with cisplatin on hela cells proliferation
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2020
url	http://eprints.usm.my/47996/1/33.%20Thesis_Final%20Copy_THESIS_NURUL%20NADIA%20BINTI%20MOHAMAD%20ZAMBERI_P-SKM0035_19-24%20pages.pdf
_version_	1747821861741264896

Metastatic inhibitory effect of gallic acid combined with cisplatin on hela cells proliferation

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