Effect of accelerated portland cement-chitosan on dentinogenic/osteogenic differentiation of stem cell from human exfoliated deciduous teeth

Mineral trioxide aggregate (MTA) is a recent gold standard endodontic biomaterial but its application is limited by expensive cost, long setting time and can cause cell toxicity. To overcome this problem, the discovery and development of new potential biomaterial is crucially required. Hence, in...

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Main Author:	Yasin, Zahidah Nasuha Mohd
Format:	Thesis
Language:	English
Published:	2020
Subjects:	R Medicine
Online Access:	http://eprints.usm.my/48001/1/37.%20Thesis_Final%20Copy_THESIS_ZAHIDAH%20NASUHA%20BINTI%20MOHD%20YASIN_P-SKM0075_19-24%20pages.pdf
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spelling	my-usm-ep.480012020-12-16T08:04:28Z Effect of accelerated portland cement-chitosan on dentinogenic/osteogenic differentiation of stem cell from human exfoliated deciduous teeth 2020-08 Yasin, Zahidah Nasuha Mohd R Medicine Mineral trioxide aggregate (MTA) is a recent gold standard endodontic biomaterial but its application is limited by expensive cost, long setting time and can cause cell toxicity. To overcome this problem, the discovery and development of new potential biomaterial is crucially required. Hence, in this study we determined the effects of accelerated Portland cement-chitosan (APC-CHT) on the dentinogenic and osteogenic differentiation in stem cells from human exfoliated deciduous teeth (SHED). The APC-CHT was prepared 1 week prior to the treatment on SHED for 14 days. The cell morphology was accessed using an inverted microscope. SHED demonstrated phenotype transition from fibroblast-like to extended-spindle morphologies starting from Day 7 until Day 14. The highest protein concentration obtained through Bradford assay was 16.89 mg/ml which is in group 4 (APC-2.5 % CHT). The sizes of proteins in this study i.e. OCN, GAPDH, ALP, DMP-1, MEPE, OPN and DSPP were evaluated using SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The highest band intensity was observed in Day 14 in group 3 (APC-1.25 % CHT) and 4 (APC-2.5 % CHT). Western blot analysis also demonstrated the appearance of apparent bands in Day 14 in both group 3 and 4. In conclusion, APC-CHT exhibited favourable effects on dentinogenic/osteogenic differentiation in SHED and has the potential to be used as an alternative material in dental applications. 2020-08 Thesis http://eprints.usm.my/48001/ http://eprints.usm.my/48001/1/37.%20Thesis_Final%20Copy_THESIS_ZAHIDAH%20NASUHA%20BINTI%20MOHD%20YASIN_P-SKM0075_19-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine
spellingShingle	R Medicine Yasin, Zahidah Nasuha Mohd Effect of accelerated portland cement-chitosan on dentinogenic/osteogenic differentiation of stem cell from human exfoliated deciduous teeth
description	Mineral trioxide aggregate (MTA) is a recent gold standard endodontic biomaterial but its application is limited by expensive cost, long setting time and can cause cell toxicity. To overcome this problem, the discovery and development of new potential biomaterial is crucially required. Hence, in this study we determined the effects of accelerated Portland cement-chitosan (APC-CHT) on the dentinogenic and osteogenic differentiation in stem cells from human exfoliated deciduous teeth (SHED). The APC-CHT was prepared 1 week prior to the treatment on SHED for 14 days. The cell morphology was accessed using an inverted microscope. SHED demonstrated phenotype transition from fibroblast-like to extended-spindle morphologies starting from Day 7 until Day 14. The highest protein concentration obtained through Bradford assay was 16.89 mg/ml which is in group 4 (APC-2.5 % CHT). The sizes of proteins in this study i.e. OCN, GAPDH, ALP, DMP-1, MEPE, OPN and DSPP were evaluated using SDS-Polyacrylamide Gel Electrophoresis (SDS-PAGE). The highest band intensity was observed in Day 14 in group 3 (APC-1.25 % CHT) and 4 (APC-2.5 % CHT). Western blot analysis also demonstrated the appearance of apparent bands in Day 14 in both group 3 and 4. In conclusion, APC-CHT exhibited favourable effects on dentinogenic/osteogenic differentiation in SHED and has the potential to be used as an alternative material in dental applications.
format	Thesis
qualification_level	Master's degree
author	Yasin, Zahidah Nasuha Mohd
author_facet	Yasin, Zahidah Nasuha Mohd
author_sort	Yasin, Zahidah Nasuha Mohd
title	Effect of accelerated portland cement-chitosan on dentinogenic/osteogenic differentiation of stem cell from human exfoliated deciduous teeth
title_short	Effect of accelerated portland cement-chitosan on dentinogenic/osteogenic differentiation of stem cell from human exfoliated deciduous teeth
title_full	Effect of accelerated portland cement-chitosan on dentinogenic/osteogenic differentiation of stem cell from human exfoliated deciduous teeth
title_fullStr	Effect of accelerated portland cement-chitosan on dentinogenic/osteogenic differentiation of stem cell from human exfoliated deciduous teeth
title_full_unstemmed	Effect of accelerated portland cement-chitosan on dentinogenic/osteogenic differentiation of stem cell from human exfoliated deciduous teeth
title_sort	effect of accelerated portland cement-chitosan on dentinogenic/osteogenic differentiation of stem cell from human exfoliated deciduous teeth
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2020
url	http://eprints.usm.my/48001/1/37.%20Thesis_Final%20Copy_THESIS_ZAHIDAH%20NASUHA%20BINTI%20MOHD%20YASIN_P-SKM0075_19-24%20pages.pdf
_version_	1747821862957613056

Effect of accelerated portland cement-chitosan on dentinogenic/osteogenic differentiation of stem cell from human exfoliated deciduous teeth

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