Cell-Based Therapy Of Human Adipose- Derived Mesenchymal Stem Cell Expressing Angiopoietin-1 In An Experimental Model Of Airway Inflammation

Cell-based therapy of mesenchymal stem cells (MSCs) has been shown to enhance the endogenous repair process by increasing the limited regenerative capacity of the lung in chronic lung disorders as well as following injury. However, the underlying cellular and molecular mechanisms of MSCs to enhance...

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主要作者: Halim, Nur Shuhaidatul Sarmiza Abdul
格式: Thesis
语言:English
出版: 2018
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在线阅读:http://eprints.usm.my/48009/1/NUR%20SHUHAIDATUL%20SARMIZA%20ABD%20HALIM%20-%20CELL-BASED%20THERAPY%20OF%20HUMAN%20ADIPOSEDERIVED%20MESENCHYMAL%20STEM%20CELL.pdf
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spelling my-usm-ep.480092020-12-21T06:52:54Z Cell-Based Therapy Of Human Adipose- Derived Mesenchymal Stem Cell Expressing Angiopoietin-1 In An Experimental Model Of Airway Inflammation 2018-09 Halim, Nur Shuhaidatul Sarmiza Abdul R735-854 Medical education. Medical schools. Research Cell-based therapy of mesenchymal stem cells (MSCs) has been shown to enhance the endogenous repair process by increasing the limited regenerative capacity of the lung in chronic lung disorders as well as following injury. However, the underlying cellular and molecular mechanisms of MSCs to enhance airway regeneration and repair are remain unclear. This study was aimed to investigate the effect of delivering human adipose-derived MSCs (hAD-MSCs) alone and in combination with vasculoprotective factor, the ANGPT1, by aerosol technique in a rabbit model of asthma related-airway inflammation. Given the anti-inflammatory, anti-permeability, and endothelial-protective characteristics of ANGPT1, we hypothesise that, combining MSCs with ANGPT1 offers promise in the treatment of airway inflammation. Transfection of hAD-MSCs with ANGPT1 was performed using microporation technique. For in vivo study, the rabbit were sensitised with combination of ovalbumin (Ova) and alum injection and further challenged with Ova inhalation to induce asthma-related airway inflammation. The MSCs and MSC-pANGPT1 were aerosolised directly into the airway using the MicroSprayer® Aerosolizer 48 h after injury. Histopathological assessments of the airway inflammation along with local inflammatory responses were quantitatively measured at three days after cell delivery. To investigate the functional effect of MSCs on airway regeneration and repair, an indirect in vitro co-culture model of injured airway epithelium explant with MSCs was developed. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed to determine the factors secreted by MSCs and their involvement in epithelium regeneration and repair was evaluated by histopathological assessment. 2018-09 Thesis http://eprints.usm.my/48009/ http://eprints.usm.my/48009/1/NUR%20SHUHAIDATUL%20SARMIZA%20ABD%20HALIM%20-%20CELL-BASED%20THERAPY%20OF%20HUMAN%20ADIPOSEDERIVED%20MESENCHYMAL%20STEM%20CELL.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R735-854 Medical education
Medical schools
Research
spellingShingle R735-854 Medical education
Medical schools
Research
Halim, Nur Shuhaidatul Sarmiza Abdul
Cell-Based Therapy Of Human Adipose- Derived Mesenchymal Stem Cell Expressing Angiopoietin-1 In An Experimental Model Of Airway Inflammation
description Cell-based therapy of mesenchymal stem cells (MSCs) has been shown to enhance the endogenous repair process by increasing the limited regenerative capacity of the lung in chronic lung disorders as well as following injury. However, the underlying cellular and molecular mechanisms of MSCs to enhance airway regeneration and repair are remain unclear. This study was aimed to investigate the effect of delivering human adipose-derived MSCs (hAD-MSCs) alone and in combination with vasculoprotective factor, the ANGPT1, by aerosol technique in a rabbit model of asthma related-airway inflammation. Given the anti-inflammatory, anti-permeability, and endothelial-protective characteristics of ANGPT1, we hypothesise that, combining MSCs with ANGPT1 offers promise in the treatment of airway inflammation. Transfection of hAD-MSCs with ANGPT1 was performed using microporation technique. For in vivo study, the rabbit were sensitised with combination of ovalbumin (Ova) and alum injection and further challenged with Ova inhalation to induce asthma-related airway inflammation. The MSCs and MSC-pANGPT1 were aerosolised directly into the airway using the MicroSprayer® Aerosolizer 48 h after injury. Histopathological assessments of the airway inflammation along with local inflammatory responses were quantitatively measured at three days after cell delivery. To investigate the functional effect of MSCs on airway regeneration and repair, an indirect in vitro co-culture model of injured airway epithelium explant with MSCs was developed. Liquid chromatography-tandem mass spectrometry (LC-MS/MS) analysis was performed to determine the factors secreted by MSCs and their involvement in epithelium regeneration and repair was evaluated by histopathological assessment.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Halim, Nur Shuhaidatul Sarmiza Abdul
author_facet Halim, Nur Shuhaidatul Sarmiza Abdul
author_sort Halim, Nur Shuhaidatul Sarmiza Abdul
title Cell-Based Therapy Of Human Adipose- Derived Mesenchymal Stem Cell Expressing Angiopoietin-1 In An Experimental Model Of Airway Inflammation
title_short Cell-Based Therapy Of Human Adipose- Derived Mesenchymal Stem Cell Expressing Angiopoietin-1 In An Experimental Model Of Airway Inflammation
title_full Cell-Based Therapy Of Human Adipose- Derived Mesenchymal Stem Cell Expressing Angiopoietin-1 In An Experimental Model Of Airway Inflammation
title_fullStr Cell-Based Therapy Of Human Adipose- Derived Mesenchymal Stem Cell Expressing Angiopoietin-1 In An Experimental Model Of Airway Inflammation
title_full_unstemmed Cell-Based Therapy Of Human Adipose- Derived Mesenchymal Stem Cell Expressing Angiopoietin-1 In An Experimental Model Of Airway Inflammation
title_sort cell-based therapy of human adipose- derived mesenchymal stem cell expressing angiopoietin-1 in an experimental model of airway inflammation
granting_institution Universiti Sains Malaysia
granting_department Institut Perubatan & Pergigian Termaju
publishDate 2018
url http://eprints.usm.my/48009/1/NUR%20SHUHAIDATUL%20SARMIZA%20ABD%20HALIM%20-%20CELL-BASED%20THERAPY%20OF%20HUMAN%20ADIPOSEDERIVED%20MESENCHYMAL%20STEM%20CELL.pdf
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