Phytochemical screening and natural killer cells immunomodulation effects of pereskia bleo leaves extract on cervical cancer cells
Pereskia bleo is a leafy and edible plant, locally known as 'Pokok Jarum Tujuh Bilah" which has anti-cancer properties. This study purposed to elucidate the underlying mechanism of this plant as anti-cancer in inducing cell death as well as to evaluate its immunostimulatory effects on N...
Saved in:
| Main Author: | |
|---|---|
| Format: | Thesis |
| Language: | English |
| Published: |
2020
|
| Subjects: | |
| Online Access: | http://eprints.usm.my/48014/1/43.%20SITI%20FARHANAH%20BINTI%20MOHD%20SALLEH-FINAL%20THESIS%20P-UD000118%28R%29%20PWD_OCR.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| id |
my-usm-ep.48014 |
|---|---|
| record_format |
uketd_dc |
| spelling |
my-usm-ep.480142020-12-21T08:39:49Z Phytochemical screening and natural killer cells immunomodulation effects of pereskia bleo leaves extract on cervical cancer cells 2020-08 Salleh, Siti Farhanah Mohd R Medicine Pereskia bleo is a leafy and edible plant, locally known as 'Pokok Jarum Tujuh Bilah" which has anti-cancer properties. This study purposed to elucidate the underlying mechanism of this plant as anti-cancer in inducing cell death as well as to evaluate its immunostimulatory effects on Natural Killer cells (NK cells) as a potential additional anti-cancer effect. In this study, the leaves of P. bleo were extracted using propenyl) and hexadecanoic acid. PBEA exhibited the lowest IC50 value (14.37 ± 8.40 μg/ml) indicated the strongest cytotoxic effect selectively on cervical cancer cells (HeLa). The cell cycle analysis showed inhibition of cell proliferation at G0/G1 phase in PBEA treated HeLa cells as evidenced by a significant accumulation of the cells at this phase (P<0.05). Morphological examination on PBEA treated HeLa cell showed the presence of fragmented nuclei and condensation of chromatin while apoptosis was detected in the Annexin V/PI assay. Analysis of apoptotic proteins revealed a significant upregulation of pro-apoptotic proteins (Bax, p53 and caspase-3) while downregulation of anti-apoptotic protein Bcl-2 (P<0.05) in PBEA treated HeLa cells. Meanwhile, NK cells proliferation at 24 h was found significantly increased compared to 48 h and 72 h of PBEA treatment (P<0.05). Apoptosis of HeLa cells was markedly increased in PBEA treated NK cells from cancer patients. This extract also enhanced granzyme B and IFN- 2020-08 Thesis http://eprints.usm.my/48014/ http://eprints.usm.my/48014/1/43.%20SITI%20FARHANAH%20BINTI%20MOHD%20SALLEH-FINAL%20THESIS%20P-UD000118%28R%29%20PWD_OCR.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Perubatan |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
R Medicine |
| spellingShingle |
R Medicine Salleh, Siti Farhanah Mohd Phytochemical screening and natural killer cells immunomodulation effects of pereskia bleo leaves extract on cervical cancer cells |
| description |
Pereskia bleo is a leafy and edible plant, locally known as 'Pokok Jarum Tujuh
Bilah" which has anti-cancer properties. This study purposed to elucidate the
underlying mechanism of this plant as anti-cancer in inducing cell death as well as to
evaluate its immunostimulatory effects on Natural Killer cells (NK cells) as a potential
additional anti-cancer effect. In this study, the leaves of P. bleo were extracted using
propenyl) and hexadecanoic acid. PBEA exhibited the lowest IC50 value (14.37 ± 8.40
μg/ml) indicated the strongest cytotoxic effect selectively on cervical cancer cells
(HeLa). The cell cycle analysis showed inhibition of cell proliferation at G0/G1 phase
in PBEA treated HeLa cells as evidenced by a significant accumulation of the cells at
this phase (P<0.05). Morphological examination on PBEA treated HeLa cell showed
the presence of fragmented nuclei and condensation of chromatin while apoptosis was
detected in the Annexin V/PI assay. Analysis of apoptotic proteins revealed a
significant upregulation of pro-apoptotic proteins (Bax, p53 and caspase-3) while
downregulation of anti-apoptotic protein Bcl-2 (P<0.05) in PBEA treated HeLa cells.
Meanwhile, NK cells proliferation at 24 h was found significantly increased compared
to 48 h and 72 h of PBEA treatment (P<0.05). Apoptosis of HeLa cells was markedly
increased in PBEA treated NK cells from cancer patients. This extract also enhanced
granzyme B and IFN- |
| format |
Thesis |
| qualification_name |
Doctor of Philosophy (PhD.) |
| qualification_level |
Doctorate |
| author |
Salleh, Siti Farhanah Mohd |
| author_facet |
Salleh, Siti Farhanah Mohd |
| author_sort |
Salleh, Siti Farhanah Mohd |
| title |
Phytochemical screening and natural killer cells immunomodulation effects of pereskia bleo leaves extract on cervical cancer cells |
| title_short |
Phytochemical screening and natural killer cells immunomodulation effects of pereskia bleo leaves extract on cervical cancer cells |
| title_full |
Phytochemical screening and natural killer cells immunomodulation effects of pereskia bleo leaves extract on cervical cancer cells |
| title_fullStr |
Phytochemical screening and natural killer cells immunomodulation effects of pereskia bleo leaves extract on cervical cancer cells |
| title_full_unstemmed |
Phytochemical screening and natural killer cells immunomodulation effects of pereskia bleo leaves extract on cervical cancer cells |
| title_sort |
phytochemical screening and natural killer cells immunomodulation effects of pereskia bleo leaves extract on cervical cancer cells |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Perubatan |
| publishDate |
2020 |
| url |
http://eprints.usm.my/48014/1/43.%20SITI%20FARHANAH%20BINTI%20MOHD%20SALLEH-FINAL%20THESIS%20P-UD000118%28R%29%20PWD_OCR.pdf |
| _version_ |
1747821865879994368 |