Structural and biochemical characterization of bees propolis

Pharmacological and clinical evidence based related chemical studies to harness local folk or traditional medicines are predominantly initiated and were derived from exploitation of geo-located local ‘status quo’ flora and botanical plants biodiversity. As such this was the basis platform of most ea...

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Main Author:	Rahim, Muhammad Kamil Abdul
Format:	Thesis
Language:	English
Published:	2020
Subjects:	R Medicine
Online Access:	http://eprints.usm.my/48087/1/58.%20MUHAMMAD%20KAMIL%20BIN%20ABDUL%20RAHIM-FINAL%20THESIS%20P-SKD000113%28R%29%20PWD_-24%20pages.pdf
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spelling	my-usm-ep.480872021-01-13T03:41:22Z Structural and biochemical characterization of bees propolis 2020-09 Rahim, Muhammad Kamil Abdul R Medicine Pharmacological and clinical evidence based related chemical studies to harness local folk or traditional medicines are predominantly initiated and were derived from exploitation of geo-located local ‘status quo’ flora and botanical plants biodiversity. As such this was the basis platform of most early medicines. Propolis is is the generic name of a natural resinous mixture produced by honey bees from substances collected from parts of plants, buds, and exudatesin different geographic areas. Propolis honeybee product have been used in global traditional folk medicine signatures for antioxidant, immune-stimulating, anti-inflammatory and anti-cancer effects. While in the industrial era, propolis application in health care has become more trending but much evidence base extrapolation still need to be explore. For acknowledgement, the paradigm concerning propolis chemistry radically changed. There are very few studies that characterize the content of Trigona spp and comparison with Apis mellifera propolis in terms of the nature of the propolis, the compound present, the safety issue and also the stability of the substances in formulation product. In this research, semi-quantitative and qualitative approaches had been used to characterize Trigona spp and Apis mellifera propolis. Trigona spp propolis was collected from Kubang Kerian, Kelantan in June 2011 and was characterized using HPTLC, GC-MS, SEM surface analysis and EDX microanalysis to determine the chemical properties and compounds present in the sample. HPTLC result showed 7 types of phenolic compounds with 3 antixodiant compounds present in Trigona spp propolis while 3 antioxidant compounds in Apis mellifera propolis. The compounds currently unidentified as the banding showed no similarities with standards used in this study (Caffeic acid, Naringenin, Naringin, Kaempferol and Luteolin). The GC- MS data detected 2 types of volatile phenolic compound (Cardol in both propolis, Cardanol in Trigona spp and1,3-Benzenediol,5-pentadecyl in Apis mellifera propolis). The in vitro evaluation showed Trigona spp contained higher phenolics compound with almost similar level of antioxidant capactiy compared to Apis mellifera propolis. Ultra-microscopy data showed presence of majority flat layer homogenized layer in both propolis where Trigona spp propolis showed more presence of compounds. Presence of more pollen, seed-like structure and microfibre wooden chip in Trigona spp propolis support chemical finding of different foraging behavior compared to Apis mellifera. Trigona spp and Apis mellifera propolis obtained in Malaysia contain phenolic compounds similar to propolis reported in Thailand and Indonesia. The propolis did not contain well known phenolic compounds commonly seen in the European country. It also contained several trace elements, and free from volatile toxic compound 2020-09 Thesis http://eprints.usm.my/48087/ http://eprints.usm.my/48087/1/58.%20MUHAMMAD%20KAMIL%20BIN%20ABDUL%20RAHIM-FINAL%20THESIS%20P-SKD000113%28R%29%20PWD_-24%20pages.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine
spellingShingle	R Medicine Rahim, Muhammad Kamil Abdul Structural and biochemical characterization of bees propolis
description	Pharmacological and clinical evidence based related chemical studies to harness local folk or traditional medicines are predominantly initiated and were derived from exploitation of geo-located local ‘status quo’ flora and botanical plants biodiversity. As such this was the basis platform of most early medicines. Propolis is is the generic name of a natural resinous mixture produced by honey bees from substances collected from parts of plants, buds, and exudatesin different geographic areas. Propolis honeybee product have been used in global traditional folk medicine signatures for antioxidant, immune-stimulating, anti-inflammatory and anti-cancer effects. While in the industrial era, propolis application in health care has become more trending but much evidence base extrapolation still need to be explore. For acknowledgement, the paradigm concerning propolis chemistry radically changed. There are very few studies that characterize the content of Trigona spp and comparison with Apis mellifera propolis in terms of the nature of the propolis, the compound present, the safety issue and also the stability of the substances in formulation product. In this research, semi-quantitative and qualitative approaches had been used to characterize Trigona spp and Apis mellifera propolis. Trigona spp propolis was collected from Kubang Kerian, Kelantan in June 2011 and was characterized using HPTLC, GC-MS, SEM surface analysis and EDX microanalysis to determine the chemical properties and compounds present in the sample. HPTLC result showed 7 types of phenolic compounds with 3 antixodiant compounds present in Trigona spp propolis while 3 antioxidant compounds in Apis mellifera propolis. The compounds currently unidentified as the banding showed no similarities with standards used in this study (Caffeic acid, Naringenin, Naringin, Kaempferol and Luteolin). The GC- MS data detected 2 types of volatile phenolic compound (Cardol in both propolis, Cardanol in Trigona spp and1,3-Benzenediol,5-pentadecyl in Apis mellifera propolis). The in vitro evaluation showed Trigona spp contained higher phenolics compound with almost similar level of antioxidant capactiy compared to Apis mellifera propolis. Ultra-microscopy data showed presence of majority flat layer homogenized layer in both propolis where Trigona spp propolis showed more presence of compounds. Presence of more pollen, seed-like structure and microfibre wooden chip in Trigona spp propolis support chemical finding of different foraging behavior compared to Apis mellifera. Trigona spp and Apis mellifera propolis obtained in Malaysia contain phenolic compounds similar to propolis reported in Thailand and Indonesia. The propolis did not contain well known phenolic compounds commonly seen in the European country. It also contained several trace elements, and free from volatile toxic compound
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Rahim, Muhammad Kamil Abdul
author_facet	Rahim, Muhammad Kamil Abdul
author_sort	Rahim, Muhammad Kamil Abdul
title	Structural and biochemical characterization of bees propolis
title_short	Structural and biochemical characterization of bees propolis
title_full	Structural and biochemical characterization of bees propolis
title_fullStr	Structural and biochemical characterization of bees propolis
title_full_unstemmed	Structural and biochemical characterization of bees propolis
title_sort	structural and biochemical characterization of bees propolis
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2020
url	http://eprints.usm.my/48087/1/58.%20MUHAMMAD%20KAMIL%20BIN%20ABDUL%20RAHIM-FINAL%20THESIS%20P-SKD000113%28R%29%20PWD_-24%20pages.pdf
_version_	1747821879071080448

Structural and biochemical characterization of bees propolis

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