Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines

Background: Breast cancer is the most common cancer affected women and the incidence rate is increasing yearly throughout the world. Tamoxifen is the first-line chemotherapeutic drug used in treating breast cancer patients. However, gynaecological complications and drug resistance are among the majo...

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Main Author:	Al Kanan, Alaa Taha Yasir
Format:	Thesis
Language:	English
Published:	2019
Subjects:	SB Plant culture
Online Access:	http://eprints.usm.my/48149/1/ALAA%20TAHA%20YASIR%20AL%20KANAN_hj.pdf
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id	my-usm-ep.48149
record_format	uketd_dc
spelling	my-usm-ep.481492021-01-20T07:58:29Z Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines 2019 Al Kanan, Alaa Taha Yasir SB Plant culture SB610-615 Weeds, parasitic plants, etc. Background: Breast cancer is the most common cancer affected women and the incidence rate is increasing yearly throughout the world. Tamoxifen is the first-line chemotherapeutic drug used in treating breast cancer patients. However, gynaecological complications and drug resistance are among the major drawback effects of tamoxifen, particularly at 10-15 years of post-treatment. Thereafter, breast cancer recurrence may occur which also contribute to the major causes of breast cancer-related deaths. Garcinia hombroniana (GH) (seashore mangosteen) is a plant that has been reported to possess good cytotoxic effect against the growth of various human cancer cell lines, including the MCF-7. For this reason, the aim of this study was to further investigate the anti-proliferative and apoptotic effects of the essential oil extracted from the leaves of GH against two different types of breast cancer cell lines, MCF-7 and MCF-7/TAMR-1. Methods: The essential oil was extracted by hydrodistillation process from the leaves of GH. The anti-proliferative effects of the essential oil against MCF-7 and MCF-7/TAMR-1 cancer cell lines were determined using MTT assay and measured by a microplate reader. The mechanism of cell death was determined using Annexin V-FITC/propidium iodide staining assay and quantitatively measured by flow cytometry. The human non-cancerous breast cell line, MCF-10A was also included in both assays as comparative control cells to the MCF-7 and MCF-7/TAMR-1 cells. 2019 Thesis http://eprints.usm.my/48149/ http://eprints.usm.my/48149/1/ALAA%20TAHA%20YASIR%20AL%20KANAN_hj.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	SB Plant culture SB Plant culture
spellingShingle	SB Plant culture SB Plant culture Al Kanan, Alaa Taha Yasir Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines
description	Background: Breast cancer is the most common cancer affected women and the incidence rate is increasing yearly throughout the world. Tamoxifen is the first-line chemotherapeutic drug used in treating breast cancer patients. However, gynaecological complications and drug resistance are among the major drawback effects of tamoxifen, particularly at 10-15 years of post-treatment. Thereafter, breast cancer recurrence may occur which also contribute to the major causes of breast cancer-related deaths. Garcinia hombroniana (GH) (seashore mangosteen) is a plant that has been reported to possess good cytotoxic effect against the growth of various human cancer cell lines, including the MCF-7. For this reason, the aim of this study was to further investigate the anti-proliferative and apoptotic effects of the essential oil extracted from the leaves of GH against two different types of breast cancer cell lines, MCF-7 and MCF-7/TAMR-1. Methods: The essential oil was extracted by hydrodistillation process from the leaves of GH. The anti-proliferative effects of the essential oil against MCF-7 and MCF-7/TAMR-1 cancer cell lines were determined using MTT assay and measured by a microplate reader. The mechanism of cell death was determined using Annexin V-FITC/propidium iodide staining assay and quantitatively measured by flow cytometry. The human non-cancerous breast cell line, MCF-10A was also included in both assays as comparative control cells to the MCF-7 and MCF-7/TAMR-1 cells.
format	Thesis
qualification_level	Master's degree
author	Al Kanan, Alaa Taha Yasir
author_facet	Al Kanan, Alaa Taha Yasir
author_sort	Al Kanan, Alaa Taha Yasir
title	Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines
title_short	Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines
title_full	Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines
title_fullStr	Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines
title_full_unstemmed	Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines
title_sort	mechanisms of anti-prolifertative effect of garcinia hombroniana essential oils leaves in mcf-7 and mcf-7/tamr-1 human breast cancer cell lines
granting_institution	Universiti Sains Malaysia
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2019
url	http://eprints.usm.my/48149/1/ALAA%20TAHA%20YASIR%20AL%20KANAN_hj.pdf
_version_	1747821889078689792

Mechanisms Of Anti-Prolifertative Effect Of Garcinia Hombroniana Essential Oils Leaves In Mcf-7 And Mcf-7/Tamr-1 Human Breast Cancer Cell Lines

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