Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans

Anthelmintic resistance has been reported in almost all species of parasites of livestock and involved all the major classes of broad-spectrum anthelmintic drug including ivermectin. Due to the study of ivermectin resistance in parasitic nematode is limited, thus, a free-living Caenorhabditis elegan...

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Main Author:	Zain, Mariani Mohd
Format:	Thesis
Language:	English
Published:	2019
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/48166/1/Mariani%20binti%20Mohd%20Zain%20cut.pdf
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id	my-usm-ep.48166
record_format	uketd_dc
spelling	my-usm-ep.481662021-01-22T01:03:27Z Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans 2019-08 Zain, Mariani Mohd QH1 Natural history (General - Including nature conservation, geographical distribution) Anthelmintic resistance has been reported in almost all species of parasites of livestock and involved all the major classes of broad-spectrum anthelmintic drug including ivermectin. Due to the study of ivermectin resistance in parasitic nematode is limited, thus, a free-living Caenorhabditis elegans was used as a model system in this study. The ivermectin sensitivity assay was carried out to investigate the response of wild-type C. elegans N2 strain to ivermectin exposure. Six in vitro assays were conducted; egg hatch assay, larval development assay, reproduce assay, thrashing assay, motility assay and pharyngeal pumping assay with IVM-resistant C. elegans DA1316 strain as a control. The results showed that ivermectin is effective on larvae but not on the eggs of wild-type C. elegans N2 strain. Ivermectin inhibits the larval development, egg-laying behavior, locomotion and feeding behavior of wild-type C. elegans N2 strain. Following the ivermectin sensitivity assay, the proteomic approach was carried out to identify the possible up-regulated and down-regulated protein expression of wild-type C. elegans N2 strain to ivermectin exposure. Two-dimensional gel electrophoresis was performed to compare the protein maps of the IVM-resistant C. elegans N2 strain (treated group) and wild-type C. elegans N2 strain (control group). Among the 25 selected protein spots, 18 protein spots were up-regulated and seven protein spots were down-regulated in the treated group compared to the control group. 2019-08 Thesis http://eprints.usm.my/48166/ http://eprints.usm.my/48166/1/Mariani%20binti%20Mohd%20Zain%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Zain, Mariani Mohd Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans
description	Anthelmintic resistance has been reported in almost all species of parasites of livestock and involved all the major classes of broad-spectrum anthelmintic drug including ivermectin. Due to the study of ivermectin resistance in parasitic nematode is limited, thus, a free-living Caenorhabditis elegans was used as a model system in this study. The ivermectin sensitivity assay was carried out to investigate the response of wild-type C. elegans N2 strain to ivermectin exposure. Six in vitro assays were conducted; egg hatch assay, larval development assay, reproduce assay, thrashing assay, motility assay and pharyngeal pumping assay with IVM-resistant C. elegans DA1316 strain as a control. The results showed that ivermectin is effective on larvae but not on the eggs of wild-type C. elegans N2 strain. Ivermectin inhibits the larval development, egg-laying behavior, locomotion and feeding behavior of wild-type C. elegans N2 strain. Following the ivermectin sensitivity assay, the proteomic approach was carried out to identify the possible up-regulated and down-regulated protein expression of wild-type C. elegans N2 strain to ivermectin exposure. Two-dimensional gel electrophoresis was performed to compare the protein maps of the IVM-resistant C. elegans N2 strain (treated group) and wild-type C. elegans N2 strain (control group). Among the 25 selected protein spots, 18 protein spots were up-regulated and seven protein spots were down-regulated in the treated group compared to the control group.
format	Thesis
qualification_level	Master's degree
author	Zain, Mariani Mohd
author_facet	Zain, Mariani Mohd
author_sort	Zain, Mariani Mohd
title	Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans
title_short	Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans
title_full	Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans
title_fullStr	Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans
title_full_unstemmed	Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans
title_sort	phenotypic and proteomic analysis associated with macrocyclic lactones-ivermectin exposure in caenorhabditis elegans
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2019
url	http://eprints.usm.my/48166/1/Mariani%20binti%20Mohd%20Zain%20cut.pdf
_version_	1747821892515921920

Phenotypic And Proteomic Analysis Associated With Macrocyclic Lactones-Ivermectin Exposure In Caenorhabditis Elegans

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