Elucidation Of The Mechanism Involved In The Proliferation And Migration Activity Of Cyperus Esculentus Lativum In Mda-Mb-231 And Mcf7 Breast Cancer Cell Lines

Cyperus esculentus L., is a tuber of family Cyperaceae which produces rhizomes from the base. It is used mostly as flour and oil in the food industry. In Ghana, it is mostly consumed raw as a snack because of its milky and sweet taste. The oil contains beta-sitosterol, oleic acid and quercetin, w...

Full description

Saved in:

Bibliographic Details
Main Author:	Seyram, Elom Achoribo
Format:	Thesis
Language:	English
Published:	2019
Subjects:	RC254-282 Neoplasms Tumors Oncology (including Cancer)
Online Access:	http://eprints.usm.my/48267/1/Seyram%20Elom%20Achoribo%20cut.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.48267
record_format	uketd_dc
spelling	my-usm-ep.482672021-02-09T01:27:43Z Elucidation Of The Mechanism Involved In The Proliferation And Migration Activity Of Cyperus Esculentus Lativum In Mda-Mb-231 And Mcf7 Breast Cancer Cell Lines 2019-07 Seyram, Elom Achoribo RC254-282 Neoplasms. Tumors. Oncology (including Cancer) Cyperus esculentus L., is a tuber of family Cyperaceae which produces rhizomes from the base. It is used mostly as flour and oil in the food industry. In Ghana, it is mostly consumed raw as a snack because of its milky and sweet taste. The oil contains beta-sitosterol, oleic acid and quercetin, which are the compounds found to have anticancer property on various cancer cell lines. This research aimed to investigate the antiproliferative effect of the ethanolic and aqueous extracts of C. esculentus on MDA-MB-231 and MCF7 breast cancer cell lines. Also, the effects of the extracts on cell cycle progression and cell migration activity of both cell lines were studied. The possible mechanisms involved in the observed activity of the extract were also elucidated. MTT assay was run to check the extract effect on the viability of both cell lines, whereas flow cytometry was used to assess the cell cycle progression. Wound healing and Matrigel assays were run to check the cell migration activity, whereas qPCR and western blotting were run for gene and protein expressions, respectively. The results show that C. esculentus extracts only reduced the cell viability to 60 % at 24 hours and 80 % at 48 hours of treatment in MDA-MB-231 and MCF7, respectively. Prolong treatment and increase concentration did not affect the cell viability further. 2019-07 Thesis http://eprints.usm.my/48267/ http://eprints.usm.my/48267/1/Seyram%20Elom%20Achoribo%20cut.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Peyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RC254-282 Neoplasms Tumors Oncology (including Cancer)
spellingShingle	RC254-282 Neoplasms Tumors Oncology (including Cancer) Seyram, Elom Achoribo Elucidation Of The Mechanism Involved In The Proliferation And Migration Activity Of Cyperus Esculentus Lativum In Mda-Mb-231 And Mcf7 Breast Cancer Cell Lines
description	Cyperus esculentus L., is a tuber of family Cyperaceae which produces rhizomes from the base. It is used mostly as flour and oil in the food industry. In Ghana, it is mostly consumed raw as a snack because of its milky and sweet taste. The oil contains beta-sitosterol, oleic acid and quercetin, which are the compounds found to have anticancer property on various cancer cell lines. This research aimed to investigate the antiproliferative effect of the ethanolic and aqueous extracts of C. esculentus on MDA-MB-231 and MCF7 breast cancer cell lines. Also, the effects of the extracts on cell cycle progression and cell migration activity of both cell lines were studied. The possible mechanisms involved in the observed activity of the extract were also elucidated. MTT assay was run to check the extract effect on the viability of both cell lines, whereas flow cytometry was used to assess the cell cycle progression. Wound healing and Matrigel assays were run to check the cell migration activity, whereas qPCR and western blotting were run for gene and protein expressions, respectively. The results show that C. esculentus extracts only reduced the cell viability to 60 % at 24 hours and 80 % at 48 hours of treatment in MDA-MB-231 and MCF7, respectively. Prolong treatment and increase concentration did not affect the cell viability further.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Seyram, Elom Achoribo
author_facet	Seyram, Elom Achoribo
author_sort	Seyram, Elom Achoribo
title	Elucidation Of The Mechanism Involved In The Proliferation And Migration Activity Of Cyperus Esculentus Lativum In Mda-Mb-231 And Mcf7 Breast Cancer Cell Lines
title_short	Elucidation Of The Mechanism Involved In The Proliferation And Migration Activity Of Cyperus Esculentus Lativum In Mda-Mb-231 And Mcf7 Breast Cancer Cell Lines
title_full	Elucidation Of The Mechanism Involved In The Proliferation And Migration Activity Of Cyperus Esculentus Lativum In Mda-Mb-231 And Mcf7 Breast Cancer Cell Lines
title_fullStr	Elucidation Of The Mechanism Involved In The Proliferation And Migration Activity Of Cyperus Esculentus Lativum In Mda-Mb-231 And Mcf7 Breast Cancer Cell Lines
title_full_unstemmed	Elucidation Of The Mechanism Involved In The Proliferation And Migration Activity Of Cyperus Esculentus Lativum In Mda-Mb-231 And Mcf7 Breast Cancer Cell Lines
title_sort	elucidation of the mechanism involved in the proliferation and migration activity of cyperus esculentus lativum in mda-mb-231 and mcf7 breast cancer cell lines
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Peyelidikan Perubatan Molekul
publishDate	2019
url	http://eprints.usm.my/48267/1/Seyram%20Elom%20Achoribo%20cut.pdf
_version_	1747821912623415296

Elucidation Of The Mechanism Involved In The Proliferation And Migration Activity Of Cyperus Esculentus Lativum In Mda-Mb-231 And Mcf7 Breast Cancer Cell Lines

Similar Items