Cryopreservation Of Oncidium Golden Anniversary’s Protocorm-Like Bodies Using Encapsulation-Dehydration Method

Oncidium Golden Anniversary is an orchid hybrid that has high economic value in flower market worldwide. However, this new orchid hybrid is difficult to maintain and cultivate conventionally thus increase the risk of permanent loss. Therefore, cryopreservation technique has been developed for long-t...

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Main Author:	Zakaria, Suhana
Format:	Thesis
Language:	English
Published:	2019
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/48307/1/Suhana%20Zakaria%20PBD000612%28R%29%20cut.pdf
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id	my-usm-ep.48307
record_format	uketd_dc
spelling	my-usm-ep.483072021-02-15T04:27:25Z Cryopreservation Of Oncidium Golden Anniversary’s Protocorm-Like Bodies Using Encapsulation-Dehydration Method 2019-06 Zakaria, Suhana QH1-278.5 Natural history (General) Oncidium Golden Anniversary is an orchid hybrid that has high economic value in flower market worldwide. However, this new orchid hybrid is difficult to maintain and cultivate conventionally thus increase the risk of permanent loss. Therefore, cryopreservation technique has been developed for long-term conservation of the orchids. This study was aimed to establish the proliferation of protocorm-like bodies (PLBs) and to develop encapsulation-dehydration method for cryopreservation of Oncidium Golden Anniversary hybrid orchid. The best media for rapid proliferation of PLBs was in half-strength semi-solid MS media supplemented with 20 g/L sucrose and 1 mg/L BAP using either whole PLBs, half-moon PLBs and transverse thin cell layer (tTCL) PLB as an explant. The PLBs produced were used as starting material in encapsulation-dehydration method and analysed by 2,3,5-triphenyltetrazolium chloride (TTC) spectrophotometry test and survivability percentage. Some important parameters have been assessed were PLB size, preculture condition, sodium alginate media, calcium chloride solution, dehydration duration, and the addition of antioxidants. 2019-06 Thesis http://eprints.usm.my/48307/ http://eprints.usm.my/48307/1/Suhana%20Zakaria%20PBD000612%28R%29%20cut.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Zakaria, Suhana Cryopreservation Of Oncidium Golden Anniversary’s Protocorm-Like Bodies Using Encapsulation-Dehydration Method
description	Oncidium Golden Anniversary is an orchid hybrid that has high economic value in flower market worldwide. However, this new orchid hybrid is difficult to maintain and cultivate conventionally thus increase the risk of permanent loss. Therefore, cryopreservation technique has been developed for long-term conservation of the orchids. This study was aimed to establish the proliferation of protocorm-like bodies (PLBs) and to develop encapsulation-dehydration method for cryopreservation of Oncidium Golden Anniversary hybrid orchid. The best media for rapid proliferation of PLBs was in half-strength semi-solid MS media supplemented with 20 g/L sucrose and 1 mg/L BAP using either whole PLBs, half-moon PLBs and transverse thin cell layer (tTCL) PLB as an explant. The PLBs produced were used as starting material in encapsulation-dehydration method and analysed by 2,3,5-triphenyltetrazolium chloride (TTC) spectrophotometry test and survivability percentage. Some important parameters have been assessed were PLB size, preculture condition, sodium alginate media, calcium chloride solution, dehydration duration, and the addition of antioxidants.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Zakaria, Suhana
author_facet	Zakaria, Suhana
author_sort	Zakaria, Suhana
title	Cryopreservation Of Oncidium Golden Anniversary’s Protocorm-Like Bodies Using Encapsulation-Dehydration Method
title_short	Cryopreservation Of Oncidium Golden Anniversary’s Protocorm-Like Bodies Using Encapsulation-Dehydration Method
title_full	Cryopreservation Of Oncidium Golden Anniversary’s Protocorm-Like Bodies Using Encapsulation-Dehydration Method
title_fullStr	Cryopreservation Of Oncidium Golden Anniversary’s Protocorm-Like Bodies Using Encapsulation-Dehydration Method
title_full_unstemmed	Cryopreservation Of Oncidium Golden Anniversary’s Protocorm-Like Bodies Using Encapsulation-Dehydration Method
title_sort	cryopreservation of oncidium golden anniversary’s protocorm-like bodies using encapsulation-dehydration method
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2019
url	http://eprints.usm.my/48307/1/Suhana%20Zakaria%20PBD000612%28R%29%20cut.pdf
_version_	1747821914322108416

Cryopreservation Of Oncidium Golden Anniversary’s Protocorm-Like Bodies Using Encapsulation-Dehydration Method

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