Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis

Eurycoma longifolia Jack roots, traditionally used as herbal remedies for increasing libido and better health conditions, contain a high content of quassinoids, the bitter substances and alkaloidal canthinones, indigenous in the Simaroubaceae family that are responsible for the plant pharmacological...

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Main Author:	Chung, Wan Jie
Format:	Thesis
Language:	English
Published:	2018
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/48310/1/CHUNG%20WAN%20JIE_hj.pdf
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spelling	my-usm-ep.483102021-02-16T06:24:16Z Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis 2018-09 Chung, Wan Jie R735-854 Medical education. Medical schools. Research Eurycoma longifolia Jack roots, traditionally used as herbal remedies for increasing libido and better health conditions, contain a high content of quassinoids, the bitter substances and alkaloidal canthinones, indigenous in the Simaroubaceae family that are responsible for the plant pharmacological properties. Amongst the quassinoids, the 20-carbon eurycomanone (EN), 13α(21)-epoxyeurycomanone (EP), eurycomanol (EL) and 13α,21-dihydroeurycomanone (DHY), possess anti-malarial, anti-ulcer, anti-pyretic, anti-osteoporosis, aphrodisiac and anti-infertility properties. In the present study, the chromatographic separation employing macroporous resin Diaion HP-20, Sepabeads SP-700 and SP-207 were evaluated for the enrichment of EN and DHY in E. longifolia methanolic extract. Results showed that among the three macroporous resin, Diaion HP-20 performed the best enrichment and separation characteristics. After single chromatographic fractionation with Diaion HP-20, the content of EN and DHY were increased from 6.13 % and 0.54 % to 30.40 % to 13.96 % w/w, respectively. Meanwhile, a simple and reliable high performance liquid chromatography with ultraviolet detection method was developed and validated for the determination of EN and DHY. Subsequently, the EN-rich and DHY-rich fractions were subjected to pharmacokinetic evaluation in rats. The present finding indicated that DHY was able to achieve a higher peak concentration (Cmax) and total area under the curve (AUC0-∞) compared to those of EN in oral dosing. 2018-09 Thesis http://eprints.usm.my/48310/ http://eprints.usm.my/48310/1/CHUNG%20WAN%20JIE_hj.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Farmasi
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Chung, Wan Jie Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis
description	Eurycoma longifolia Jack roots, traditionally used as herbal remedies for increasing libido and better health conditions, contain a high content of quassinoids, the bitter substances and alkaloidal canthinones, indigenous in the Simaroubaceae family that are responsible for the plant pharmacological properties. Amongst the quassinoids, the 20-carbon eurycomanone (EN), 13α(21)-epoxyeurycomanone (EP), eurycomanol (EL) and 13α,21-dihydroeurycomanone (DHY), possess anti-malarial, anti-ulcer, anti-pyretic, anti-osteoporosis, aphrodisiac and anti-infertility properties. In the present study, the chromatographic separation employing macroporous resin Diaion HP-20, Sepabeads SP-700 and SP-207 were evaluated for the enrichment of EN and DHY in E. longifolia methanolic extract. Results showed that among the three macroporous resin, Diaion HP-20 performed the best enrichment and separation characteristics. After single chromatographic fractionation with Diaion HP-20, the content of EN and DHY were increased from 6.13 % and 0.54 % to 30.40 % to 13.96 % w/w, respectively. Meanwhile, a simple and reliable high performance liquid chromatography with ultraviolet detection method was developed and validated for the determination of EN and DHY. Subsequently, the EN-rich and DHY-rich fractions were subjected to pharmacokinetic evaluation in rats. The present finding indicated that DHY was able to achieve a higher peak concentration (Cmax) and total area under the curve (AUC0-∞) compared to those of EN in oral dosing.
format	Thesis
qualification_level	Master's degree
author	Chung, Wan Jie
author_facet	Chung, Wan Jie
author_sort	Chung, Wan Jie
title	Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis
title_short	Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis
title_full	Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis
title_fullStr	Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis
title_full_unstemmed	Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis
title_sort	chromatographic enrichment of bioactive quassinoids in tongkat ali for the improvement of spermatogenesis
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Farmasi
publishDate	2018
url	http://eprints.usm.my/48310/1/CHUNG%20WAN%20JIE_hj.pdf
_version_	1747821915056111616

Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis

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