Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis
Eurycoma longifolia Jack roots, traditionally used as herbal remedies for increasing libido and better health conditions, contain a high content of quassinoids, the bitter substances and alkaloidal canthinones, indigenous in the Simaroubaceae family that are responsible for the plant pharmacological...
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my-usm-ep.483102021-02-16T06:24:16Z Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis 2018-09 Chung, Wan Jie R735-854 Medical education. Medical schools. Research Eurycoma longifolia Jack roots, traditionally used as herbal remedies for increasing libido and better health conditions, contain a high content of quassinoids, the bitter substances and alkaloidal canthinones, indigenous in the Simaroubaceae family that are responsible for the plant pharmacological properties. Amongst the quassinoids, the 20-carbon eurycomanone (EN), 13α(21)-epoxyeurycomanone (EP), eurycomanol (EL) and 13α,21-dihydroeurycomanone (DHY), possess anti-malarial, anti-ulcer, anti-pyretic, anti-osteoporosis, aphrodisiac and anti-infertility properties. In the present study, the chromatographic separation employing macroporous resin Diaion HP-20, Sepabeads SP-700 and SP-207 were evaluated for the enrichment of EN and DHY in E. longifolia methanolic extract. Results showed that among the three macroporous resin, Diaion HP-20 performed the best enrichment and separation characteristics. After single chromatographic fractionation with Diaion HP-20, the content of EN and DHY were increased from 6.13 % and 0.54 % to 30.40 % to 13.96 % w/w, respectively. Meanwhile, a simple and reliable high performance liquid chromatography with ultraviolet detection method was developed and validated for the determination of EN and DHY. Subsequently, the EN-rich and DHY-rich fractions were subjected to pharmacokinetic evaluation in rats. The present finding indicated that DHY was able to achieve a higher peak concentration (Cmax) and total area under the curve (AUC0-∞) compared to those of EN in oral dosing. 2018-09 Thesis http://eprints.usm.my/48310/ http://eprints.usm.my/48310/1/CHUNG%20WAN%20JIE_hj.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Farmasi |
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| language |
English |
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R735-854 Medical education Medical schools Research |
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R735-854 Medical education Medical schools Research Chung, Wan Jie Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis |
| description |
Eurycoma longifolia Jack roots, traditionally used as herbal remedies for increasing libido and better health conditions, contain a high content of quassinoids, the bitter substances and alkaloidal canthinones, indigenous in the Simaroubaceae family that are responsible for the plant pharmacological properties. Amongst the quassinoids, the 20-carbon eurycomanone (EN), 13α(21)-epoxyeurycomanone (EP), eurycomanol (EL) and 13α,21-dihydroeurycomanone (DHY), possess anti-malarial, anti-ulcer, anti-pyretic, anti-osteoporosis, aphrodisiac and anti-infertility properties. In the present study, the chromatographic separation employing macroporous resin Diaion HP-20, Sepabeads SP-700 and SP-207 were evaluated for the enrichment of EN and DHY in E. longifolia methanolic extract. Results showed that among the three macroporous resin, Diaion HP-20 performed the best enrichment and separation characteristics. After single chromatographic fractionation with Diaion HP-20, the content of EN and DHY were increased from 6.13 % and 0.54 % to 30.40 % to 13.96 % w/w, respectively. Meanwhile, a simple and reliable high performance liquid chromatography with ultraviolet detection method was developed and validated for the determination of EN and DHY. Subsequently, the EN-rich and DHY-rich fractions were subjected to pharmacokinetic evaluation in rats. The present finding indicated that DHY was able to achieve a higher peak concentration (Cmax) and total area under the curve (AUC0-∞) compared to those of EN in oral dosing. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Chung, Wan Jie |
| author_facet |
Chung, Wan Jie |
| author_sort |
Chung, Wan Jie |
| title |
Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis |
| title_short |
Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis |
| title_full |
Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis |
| title_fullStr |
Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis |
| title_full_unstemmed |
Chromatographic Enrichment Of Bioactive Quassinoids In Tongkat Ali For The Improvement Of Spermatogenesis |
| title_sort |
chromatographic enrichment of bioactive quassinoids in tongkat ali for the improvement of spermatogenesis |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Farmasi |
| publishDate |
2018 |
| url |
http://eprints.usm.my/48310/1/CHUNG%20WAN%20JIE_hj.pdf |
| _version_ |
1747821915056111616 |