Determination of lymphocyte subjects in patients with major depressive disorder (MDD)

Major depressive disorder (MDD) has been associated with dysregulation of immune system. While many studies on activation of innate immune response currently dominates the research area, the dysregulation in adaptive immune system especially in circulating lymphocyte subsets has rarely been explo...

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Main Author:	Sanusi, Siti Nor Fairus Mohamed
Format:	Thesis
Language:	English
Published:	2018
Subjects:	RM Therapeutics Pharmacology
Online Access:	http://eprints.usm.my/48525/1/Dr.%20Siti%20Nur%20Fairus-24%20pages.pdf
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spelling	my-usm-ep.485252021-03-03T04:25:39Z Determination of lymphocyte subjects in patients with major depressive disorder (MDD) 2018-01 Sanusi, Siti Nor Fairus Mohamed RM Therapeutics. Pharmacology Major depressive disorder (MDD) has been associated with dysregulation of immune system. While many studies on activation of innate immune response currently dominates the research area, the dysregulation in adaptive immune system especially in circulating lymphocyte subsets has rarely been explored. Some studies indicated that the severity of MDD is important with respect to the extent of the immune changes in MDD patients. The objective of this study was to determine the predisposing factors of MDD and the common symptoms in MDD patients based on Beck Depression Inventory (BDI) and Montgomery-Asberg Depression Rating Scale (MADRS), and to determine the percentage and absolute count of lymphocyte subsets in MDD patients and their comparison between different severity of the disease. This study involved 47 MDD patients recruited from Psychiatric Clinic, Hospital Universiti Sains Malaysia (HUSM) and 47 healthy controls. MDD patients were diagnosed according to Diagnostic and Statistical Manual of Mental Disorders Fifth Edition (DSM-V) criteria. The symptoms and severity of MDD was assessed using BDI and MADRS scale. The percentage and absolute count of CD4+ T cells, CD8+ T cell, CD4+ CD25+ Tregs, CD4+ CD25+ Foxp3+ Tregs, CD16+ CD56+ NK cells and CD19+ B cells were determined by using immunophenotyping technique. Mann-Whitney test was used to compare the percentage and absolute count of lymphocyte subsets between MDD patients and healthy controls. Kruskal-Wallis test was used to compare the percentage and absolute count of lymphocyte subsets between different severity of MDD. The results showed that those married, smoker, possess lower educational level and living in rural area have higher risk for MDD. Based on BDI, the most common symptoms reported by our MDD patients were fatigue and irritability. While based on MADRS, MDD patients also have inner tension, concentration difficulties, inability to feel and pessimistic thought. This study showed that there were no significant differences in the percentage and absolute count of CD4+ T cells (p=0.148; p=0.190), CD8+ T cells (p=0.316; p=0.783), CD16+ CD56+ NK cells (p=0.731; p=0.530), and CD19+ B cells (p=0.136; p=0.148) between MDD patients and healthy controls. However, we found a significatly higher percentage and absolute count of CD4+ CD25+ Tregs (p<0.001) and CD4+ CD25+ Foxp3+ Treg cells (p=0.003; p=0.001) in MDD patients compared with healthy controls. This study also showed that there were no significant differences in the percentage and absolute count of lymphocyte subsets between mild, moderate and severe MDD patients. 2018-01 Thesis http://eprints.usm.my/48525/ http://eprints.usm.my/48525/1/Dr.%20Siti%20Nur%20Fairus-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RM Therapeutics Pharmacology
spellingShingle	RM Therapeutics Pharmacology Sanusi, Siti Nor Fairus Mohamed Determination of lymphocyte subjects in patients with major depressive disorder (MDD)
description	Major depressive disorder (MDD) has been associated with dysregulation of immune system. While many studies on activation of innate immune response currently dominates the research area, the dysregulation in adaptive immune system especially in circulating lymphocyte subsets has rarely been explored. Some studies indicated that the severity of MDD is important with respect to the extent of the immune changes in MDD patients. The objective of this study was to determine the predisposing factors of MDD and the common symptoms in MDD patients based on Beck Depression Inventory (BDI) and Montgomery-Asberg Depression Rating Scale (MADRS), and to determine the percentage and absolute count of lymphocyte subsets in MDD patients and their comparison between different severity of the disease. This study involved 47 MDD patients recruited from Psychiatric Clinic, Hospital Universiti Sains Malaysia (HUSM) and 47 healthy controls. MDD patients were diagnosed according to Diagnostic and Statistical Manual of Mental Disorders Fifth Edition (DSM-V) criteria. The symptoms and severity of MDD was assessed using BDI and MADRS scale. The percentage and absolute count of CD4+ T cells, CD8+ T cell, CD4+ CD25+ Tregs, CD4+ CD25+ Foxp3+ Tregs, CD16+ CD56+ NK cells and CD19+ B cells were determined by using immunophenotyping technique. Mann-Whitney test was used to compare the percentage and absolute count of lymphocyte subsets between MDD patients and healthy controls. Kruskal-Wallis test was used to compare the percentage and absolute count of lymphocyte subsets between different severity of MDD. The results showed that those married, smoker, possess lower educational level and living in rural area have higher risk for MDD. Based on BDI, the most common symptoms reported by our MDD patients were fatigue and irritability. While based on MADRS, MDD patients also have inner tension, concentration difficulties, inability to feel and pessimistic thought. This study showed that there were no significant differences in the percentage and absolute count of CD4+ T cells (p=0.148; p=0.190), CD8+ T cells (p=0.316; p=0.783), CD16+ CD56+ NK cells (p=0.731; p=0.530), and CD19+ B cells (p=0.136; p=0.148) between MDD patients and healthy controls. However, we found a significatly higher percentage and absolute count of CD4+ CD25+ Tregs (p<0.001) and CD4+ CD25+ Foxp3+ Treg cells (p=0.003; p=0.001) in MDD patients compared with healthy controls. This study also showed that there were no significant differences in the percentage and absolute count of lymphocyte subsets between mild, moderate and severe MDD patients.
format	Thesis
qualification_level	Master's degree
author	Sanusi, Siti Nor Fairus Mohamed
author_facet	Sanusi, Siti Nor Fairus Mohamed
author_sort	Sanusi, Siti Nor Fairus Mohamed
title	Determination of lymphocyte subjects in patients with major depressive disorder (MDD)
title_short	Determination of lymphocyte subjects in patients with major depressive disorder (MDD)
title_full	Determination of lymphocyte subjects in patients with major depressive disorder (MDD)
title_fullStr	Determination of lymphocyte subjects in patients with major depressive disorder (MDD)
title_full_unstemmed	Determination of lymphocyte subjects in patients with major depressive disorder (MDD)
title_sort	determination of lymphocyte subjects in patients with major depressive disorder (mdd)
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2018
url	http://eprints.usm.my/48525/1/Dr.%20Siti%20Nur%20Fairus-24%20pages.pdf
_version_	1747821940120223744

Determination of lymphocyte subjects in patients with major depressive disorder (MDD)

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