Evaluation Of Antibacterial Synergy Of Polyalthia Longifolia (Sonn.) Leaf Ethyl Acetate Fraction (Pleaf) With Ampicillin Against Methicillin-Resistant Staphylococcus Aureus (Mrsa)

Polyalthia longifolia which originates from India is rich with various useful phytochemicals which are valuable for human health. The aim of the study is to evaluate whether the combination of Polyalthia longifolia ethyl acetate fraction (PLEAF) and ampicillin exhibits synergistic antimicrobial acti...

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Main Author:	Balasupramaniam, Kirubakari
Format:	Thesis
Language:	English
Published:	2019
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/48613/1/thesis%20%28verified%29new%20cut.pdf
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id	my-usm-ep.48613
record_format	uketd_dc
spelling	my-usm-ep.486132021-03-11T08:42:53Z Evaluation Of Antibacterial Synergy Of Polyalthia Longifolia (Sonn.) Leaf Ethyl Acetate Fraction (Pleaf) With Ampicillin Against Methicillin-Resistant Staphylococcus Aureus (Mrsa) 2019-03 Balasupramaniam, Kirubakari R5-920 Medicine (General) Polyalthia longifolia which originates from India is rich with various useful phytochemicals which are valuable for human health. The aim of the study is to evaluate whether the combination of Polyalthia longifolia ethyl acetate fraction (PLEAF) and ampicillin exhibits synergistic antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) bacteria followed by their antioxidant capacity and their cytotoxicity level. The evaluation of synergistic activity of PLEAF fraction and ampicillin against MRSA local isolate was conducted with various antimicrobial assays namely disc diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), fractional inhibitory concentration (FIC), time-kill study and scanning electron microscopy (SEM). The combinational effect of PLEAF fraction and ampicillin exhibited significant antibacterial activity against MRSA. This is proven when the MIC values of PLEAF fraction (62.5 μg/mL) and ampicillin (5000 μg/mL) were found to decrease to 15.63 μg/mL for PLEAF and 2500 μg/mL for ampicillin respectively in the FIC assay against the MRSA bacteria. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide free radical scavenging activities showed that PLEAF fraction possessed high antioxidant activity and the combinational of PLEAF fraction and ampicillin exhibited moderate antioxidant activity. The usage of PLEAF fraction with high antioxidants activity is important to prevent the adverse effect of the production of an excessive amount of free radicals during microbial infection. 2019-03 Thesis http://eprints.usm.my/48613/ http://eprints.usm.my/48613/1/thesis%20%28verified%29new%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Balasupramaniam, Kirubakari Evaluation Of Antibacterial Synergy Of Polyalthia Longifolia (Sonn.) Leaf Ethyl Acetate Fraction (Pleaf) With Ampicillin Against Methicillin-Resistant Staphylococcus Aureus (Mrsa)
description	Polyalthia longifolia which originates from India is rich with various useful phytochemicals which are valuable for human health. The aim of the study is to evaluate whether the combination of Polyalthia longifolia ethyl acetate fraction (PLEAF) and ampicillin exhibits synergistic antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA) bacteria followed by their antioxidant capacity and their cytotoxicity level. The evaluation of synergistic activity of PLEAF fraction and ampicillin against MRSA local isolate was conducted with various antimicrobial assays namely disc diffusion method, minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), fractional inhibitory concentration (FIC), time-kill study and scanning electron microscopy (SEM). The combinational effect of PLEAF fraction and ampicillin exhibited significant antibacterial activity against MRSA. This is proven when the MIC values of PLEAF fraction (62.5 μg/mL) and ampicillin (5000 μg/mL) were found to decrease to 15.63 μg/mL for PLEAF and 2500 μg/mL for ampicillin respectively in the FIC assay against the MRSA bacteria. The 2,2-diphenyl-1-picrylhydrazyl (DPPH) and nitric oxide free radical scavenging activities showed that PLEAF fraction possessed high antioxidant activity and the combinational of PLEAF fraction and ampicillin exhibited moderate antioxidant activity. The usage of PLEAF fraction with high antioxidants activity is important to prevent the adverse effect of the production of an excessive amount of free radicals during microbial infection.
format	Thesis
qualification_level	Master's degree
author	Balasupramaniam, Kirubakari
author_facet	Balasupramaniam, Kirubakari
author_sort	Balasupramaniam, Kirubakari
title	Evaluation Of Antibacterial Synergy Of Polyalthia Longifolia (Sonn.) Leaf Ethyl Acetate Fraction (Pleaf) With Ampicillin Against Methicillin-Resistant Staphylococcus Aureus (Mrsa)
title_short	Evaluation Of Antibacterial Synergy Of Polyalthia Longifolia (Sonn.) Leaf Ethyl Acetate Fraction (Pleaf) With Ampicillin Against Methicillin-Resistant Staphylococcus Aureus (Mrsa)
title_full	Evaluation Of Antibacterial Synergy Of Polyalthia Longifolia (Sonn.) Leaf Ethyl Acetate Fraction (Pleaf) With Ampicillin Against Methicillin-Resistant Staphylococcus Aureus (Mrsa)
title_fullStr	Evaluation Of Antibacterial Synergy Of Polyalthia Longifolia (Sonn.) Leaf Ethyl Acetate Fraction (Pleaf) With Ampicillin Against Methicillin-Resistant Staphylococcus Aureus (Mrsa)
title_full_unstemmed	Evaluation Of Antibacterial Synergy Of Polyalthia Longifolia (Sonn.) Leaf Ethyl Acetate Fraction (Pleaf) With Ampicillin Against Methicillin-Resistant Staphylococcus Aureus (Mrsa)
title_sort	evaluation of antibacterial synergy of polyalthia longifolia (sonn.) leaf ethyl acetate fraction (pleaf) with ampicillin against methicillin-resistant staphylococcus aureus (mrsa)
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2019
url	http://eprints.usm.my/48613/1/thesis%20%28verified%29new%20cut.pdf
_version_	1747821952342425600

Evaluation Of Antibacterial Synergy Of Polyalthia Longifolia (Sonn.) Leaf Ethyl Acetate Fraction (Pleaf) With Ampicillin Against Methicillin-Resistant Staphylococcus Aureus (Mrsa)

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