Enhanced Rhamnolipid Production From Waste Cooking Oil By Pseudomonas Aeruginosa Usm-Ar2

Rhamnolipid, a glycolipid type of biosurfactant is the most investigated glycolipid biosurfactant. The problem of this study was the waste cooking oil used as a major carbon source is immiscible in aqueous phase and inhibited the growth of the microorganisms studied which is Pseudomonas aeruginosa U...

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Main Author:	Samsu, Zainatul `Asyiqin
Format:	Thesis
Language:	English
Published:	2019
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/48614/1/Thesis%20correction%20completed%2022032019%20cut.pdf
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id	my-usm-ep.48614
record_format	uketd_dc
spelling	my-usm-ep.486142021-03-11T09:14:53Z Enhanced Rhamnolipid Production From Waste Cooking Oil By Pseudomonas Aeruginosa Usm-Ar2 2019-03 Samsu, Zainatul `Asyiqin QH1-278.5 Natural history (General) Rhamnolipid, a glycolipid type of biosurfactant is the most investigated glycolipid biosurfactant. The problem of this study was the waste cooking oil used as a major carbon source is immiscible in aqueous phase and inhibited the growth of the microorganisms studied which is Pseudomonas aeruginosa USM-AR2. The ultimate aim is to enhance microbial production of rhamnolipid on a lab-scale. Thus, the appropriate feeding strategy for fed-batch culture needs to be determined to increase the availability and subsequent intake of the carbon source by the microorganisms. Several objectives have to be met to ensure this strategy is achievable, which include: 1) to evaluate and select different medium formulation from literature.; 2) to determine the effect of operational conditions on rhamnolipid production and the behaviour of oxygen transfer in batch culture; 3) to analyse the kinetics of rhamnolipid production in batch culture; and 4) to identify the best feeding strategy to improve rhamnolipid production in fed-batch culture. Results showed that the modified medium composition to support rhamnolipid production contained the following: NO3-, Mg+, K+, PO43-, trace elements and waste cooking oil with C/N equivalent to 18. The addition of Tween 80, a commercial surfactant, into the medium showed no significant impact on rhamnolipid production. In a bench-top bioreactor, the agitator tip speed affected rhamnolipid production. Rhamnolipid production at a lower tip speed (1.131 m/s) was 1.5-fold higher than production at a higher tip speed (1.414 m/s). Rhamnolipid production achieved the maximum concentration of 4.86 g/L (0.041 g/L.h of the overall productivity) when the production medium was controlled at pH 6.85. 2019-03 Thesis http://eprints.usm.my/48614/ http://eprints.usm.my/48614/1/Thesis%20correction%20completed%2022032019%20cut.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Samsu, Zainatul `Asyiqin Enhanced Rhamnolipid Production From Waste Cooking Oil By Pseudomonas Aeruginosa Usm-Ar2
description	Rhamnolipid, a glycolipid type of biosurfactant is the most investigated glycolipid biosurfactant. The problem of this study was the waste cooking oil used as a major carbon source is immiscible in aqueous phase and inhibited the growth of the microorganisms studied which is Pseudomonas aeruginosa USM-AR2. The ultimate aim is to enhance microbial production of rhamnolipid on a lab-scale. Thus, the appropriate feeding strategy for fed-batch culture needs to be determined to increase the availability and subsequent intake of the carbon source by the microorganisms. Several objectives have to be met to ensure this strategy is achievable, which include: 1) to evaluate and select different medium formulation from literature.; 2) to determine the effect of operational conditions on rhamnolipid production and the behaviour of oxygen transfer in batch culture; 3) to analyse the kinetics of rhamnolipid production in batch culture; and 4) to identify the best feeding strategy to improve rhamnolipid production in fed-batch culture. Results showed that the modified medium composition to support rhamnolipid production contained the following: NO3-, Mg+, K+, PO43-, trace elements and waste cooking oil with C/N equivalent to 18. The addition of Tween 80, a commercial surfactant, into the medium showed no significant impact on rhamnolipid production. In a bench-top bioreactor, the agitator tip speed affected rhamnolipid production. Rhamnolipid production at a lower tip speed (1.131 m/s) was 1.5-fold higher than production at a higher tip speed (1.414 m/s). Rhamnolipid production achieved the maximum concentration of 4.86 g/L (0.041 g/L.h of the overall productivity) when the production medium was controlled at pH 6.85.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Samsu, Zainatul `Asyiqin
author_facet	Samsu, Zainatul `Asyiqin
author_sort	Samsu, Zainatul `Asyiqin
title	Enhanced Rhamnolipid Production From Waste Cooking Oil By Pseudomonas Aeruginosa Usm-Ar2
title_short	Enhanced Rhamnolipid Production From Waste Cooking Oil By Pseudomonas Aeruginosa Usm-Ar2
title_full	Enhanced Rhamnolipid Production From Waste Cooking Oil By Pseudomonas Aeruginosa Usm-Ar2
title_fullStr	Enhanced Rhamnolipid Production From Waste Cooking Oil By Pseudomonas Aeruginosa Usm-Ar2
title_full_unstemmed	Enhanced Rhamnolipid Production From Waste Cooking Oil By Pseudomonas Aeruginosa Usm-Ar2
title_sort	enhanced rhamnolipid production from waste cooking oil by pseudomonas aeruginosa usm-ar2
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2019
url	http://eprints.usm.my/48614/1/Thesis%20correction%20completed%2022032019%20cut.pdf
_version_	1747821952585695232

Enhanced Rhamnolipid Production From Waste Cooking Oil By Pseudomonas Aeruginosa Usm-Ar2

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