Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson’s Disease Models

Human menstrual blood-derived endometrial stem cells (MenSCs) have shown therapeutic potential on various diseases by immunoregulation and tissue regeneration. However, their effects on Parkinson’s disease (PD) remain unknown. The aim of this study was to evaluate the protective function of MenSCs a...

Full description

Saved in:

Bibliographic Details
Main Author:	Li, Han
Format:	Thesis
Language:	English
Published:	2019
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/49079/1/Li%20Han%20thesis%20-%20final%20%20version%20for%20submition%20cut.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.49079
record_format	uketd_dc
spelling	my-usm-ep.490792021-05-03T08:13:03Z Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson’s Disease Models 2019-08 Li, Han R5-920 Medicine (General) Human menstrual blood-derived endometrial stem cells (MenSCs) have shown therapeutic potential on various diseases by immunoregulation and tissue regeneration. However, their effects on Parkinson’s disease (PD) remain unknown. The aim of this study was to evaluate the protective function of MenSCs and their derivatives on in vitro and in vivo PD models. Neuroblastoma cell line (SH-SY5Y) and mouse midbrain slice were exposed to 1-methyl-4-phenylpyridinium (MPP+) to establish in vitro level PD models. Then, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was used to construct PD mouse model by intraperitoneal injection. After co-culture in vitro PD models with conditioned medium of MenSCs (MenSCs-CM), the viability of cell and midbrain slice were detected by Prestoblue and lactate dehydrogenase assay. The expression of inflammatory genes, anti-oxidant and apoptosis-related genes were detected by qRT-PCR. Dihydroethidium, Rhodamine123, and Annexin V/PI staining were used to detect reactive oxygen species (ROS), mitochondrial membrane potential, and cell apoptosis, respectively. Protein array was conducted to analyze factors inside MenSCs-CM. Moreover, MenSCs were transplanted to striatum (Str) region of PD mouse brain using a stereotaxic instrument. Survival time of MenSCs, dopamine (DA) level, expression of inflammatory genes and anti-oxidant genes were evaluated. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was used to analyze pathways. 2019-08 Thesis http://eprints.usm.my/49079/ http://eprints.usm.my/49079/1/Li%20Han%20thesis%20-%20final%20%20version%20for%20submition%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Li, Han Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson’s Disease Models
description	Human menstrual blood-derived endometrial stem cells (MenSCs) have shown therapeutic potential on various diseases by immunoregulation and tissue regeneration. However, their effects on Parkinson’s disease (PD) remain unknown. The aim of this study was to evaluate the protective function of MenSCs and their derivatives on in vitro and in vivo PD models. Neuroblastoma cell line (SH-SY5Y) and mouse midbrain slice were exposed to 1-methyl-4-phenylpyridinium (MPP+) to establish in vitro level PD models. Then, 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) was used to construct PD mouse model by intraperitoneal injection. After co-culture in vitro PD models with conditioned medium of MenSCs (MenSCs-CM), the viability of cell and midbrain slice were detected by Prestoblue and lactate dehydrogenase assay. The expression of inflammatory genes, anti-oxidant and apoptosis-related genes were detected by qRT-PCR. Dihydroethidium, Rhodamine123, and Annexin V/PI staining were used to detect reactive oxygen species (ROS), mitochondrial membrane potential, and cell apoptosis, respectively. Protein array was conducted to analyze factors inside MenSCs-CM. Moreover, MenSCs were transplanted to striatum (Str) region of PD mouse brain using a stereotaxic instrument. Survival time of MenSCs, dopamine (DA) level, expression of inflammatory genes and anti-oxidant genes were evaluated. Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis was used to analyze pathways.
format	Thesis
qualification_level	Master's degree
author	Li, Han
author_facet	Li, Han
author_sort	Li, Han
title	Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson’s Disease Models
title_short	Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson’s Disease Models
title_full	Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson’s Disease Models
title_fullStr	Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson’s Disease Models
title_full_unstemmed	Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson’s Disease Models
title_sort	therapeutic potential of menstrual blood-derived endometrium stem cells on in vitro and in vivo parkinson’s disease models
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2019
url	http://eprints.usm.my/49079/1/Li%20Han%20thesis%20-%20final%20%20version%20for%20submition%20cut.pdf
_version_	1747821980490399744

Therapeutic Potential Of Menstrual Blood-Derived Endometrium Stem Cells On In Vitro And In Vivo Parkinson’s Disease Models

Similar Items