Evaluation of phenol chloroform DNA extraction method from a speck blood stain on cotton fabric
In forensic casework, DNA recovery and the efficiency of DNA extraction method is one of the important key for the forensic investigator to identify the individual involved with the crime through the DNA analysis. This study therefore is intended to evaluate three parameters of phenol chloroform...
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2020
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my-usm-ep.491142021-05-06T03:57:27Z Evaluation of phenol chloroform DNA extraction method from a speck blood stain on cotton fabric 2020-09 Nazri, Nor Amalia R Medicine In forensic casework, DNA recovery and the efficiency of DNA extraction method is one of the important key for the forensic investigator to identify the individual involved with the crime through the DNA analysis. This study therefore is intended to evaluate three parameters of phenol chloroform extraction method from a tiny blood stain on fabric cotton in order to find suitable condition that can give a high DNA recovery. Highly significant of P value suggest that the selected parameters which is the incubation time, incubation temperature and storage temperature have influenced the DNA recovery. All parameters show accepted purity value at ratio A260/A280 and very low purity value for A260/A230 in all extracted samples which may be attributed by several factors. In summary, the efficiency to dissolve DNA was found to be good when sample was stored at 56ᴼC based on the tested method. 2020-09 Thesis http://eprints.usm.my/49114/ http://eprints.usm.my/49114/1/TESIS-NOR%20AMALIA%20NAZRI%20%28P-SKM0059_19%29--24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
R Medicine |
| spellingShingle |
R Medicine Nazri, Nor Amalia Evaluation of phenol chloroform DNA extraction method from a speck blood stain on cotton fabric |
| description |
In forensic casework, DNA recovery and the efficiency of DNA extraction method is
one of the important key for the forensic investigator to identify the individual involved
with the crime through the DNA analysis. This study therefore is intended to evaluate three
parameters of phenol chloroform extraction method from a tiny blood stain on fabric cotton
in order to find suitable condition that can give a high DNA recovery. Highly significant of
P value suggest that the selected parameters which is the incubation time, incubation
temperature and storage temperature have influenced the DNA recovery. All parameters
show accepted purity value at ratio A260/A280 and very low purity value for A260/A230
in all extracted samples which may be attributed by several factors. In summary, the
efficiency to dissolve DNA was found to be good when sample was stored at 56ᴼC based
on the tested method. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Nazri, Nor Amalia |
| author_facet |
Nazri, Nor Amalia |
| author_sort |
Nazri, Nor Amalia |
| title |
Evaluation of phenol chloroform DNA extraction method from a speck blood stain on cotton fabric |
| title_short |
Evaluation of phenol chloroform DNA extraction method from a speck blood stain on cotton fabric |
| title_full |
Evaluation of phenol chloroform DNA extraction method from a speck blood stain on cotton fabric |
| title_fullStr |
Evaluation of phenol chloroform DNA extraction method from a speck blood stain on cotton fabric |
| title_full_unstemmed |
Evaluation of phenol chloroform DNA extraction method from a speck blood stain on cotton fabric |
| title_sort |
evaluation of phenol chloroform dna extraction method from a speck blood stain on cotton fabric |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Kesihatan |
| publishDate |
2020 |
| url |
http://eprints.usm.my/49114/1/TESIS-NOR%20AMALIA%20NAZRI%20%28P-SKM0059_19%29--24%20pages.pdf |
| _version_ |
1747821981477109760 |