Production And Characterization Of Biosurfactant Produced By Mangrove Streptomyces Sp. Pbd-410l

Biosurfactants are microbially produced surface-active compounds that reduce the surface tension between liquid phases of differing polarity. Presently, the best reported biosurfactant producers are Pseudomonas and Bacillus spp. Unfortunately, they are known opportunistic human pathogens, with some...

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Main Author:	Rusly, Norazurin Syuhada
Format:	Thesis
Language:	English
Published:	2019
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/49130/1/NORAZURIN%20SYUHADA%20RUSLY%202019%20cut.pdf
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id	my-usm-ep.49130
record_format	uketd_dc
spelling	my-usm-ep.491302021-05-10T08:30:55Z Production And Characterization Of Biosurfactant Produced By Mangrove Streptomyces Sp. Pbd-410l 2019-08 Rusly, Norazurin Syuhada QH1-278.5 Natural history (General) Biosurfactants are microbially produced surface-active compounds that reduce the surface tension between liquid phases of differing polarity. Presently, the best reported biosurfactant producers are Pseudomonas and Bacillus spp. Unfortunately, they are known opportunistic human pathogens, with some having antibiotic-resistance, causing grave concerns. Filamentous actinobacteria can be safer alternative biosurfactant producers, with no known human pathogenicity. The present study focused on the identification of biosurfactant-producing actinobacterium, characterization of the biosurfactant produced and the effect of fermentation parameters on biosurfactant production. A total of 60 actinobacterial isolates from mangrove sediments were screened for biosurfactant production using palm oil as the main carbon source. The presence of biosurfactant was confirmed using surface tension (ST) measurement, oil spreading technique (OST) and emulsification index (E24). Isolate PBD-410L was selected as the best biosurfactant producer based on the highest value for OST (68.0±1.7 mm) and the lowest surface tension measurement (39.7±0.6 dynes/cm). This isolate was characterized and tentatively identified as Streptomyces sp. PBD-410L, based on morphological characteristics and molecular identification. Crude biosurfactant extract was successfully recovered at a concentration of 11.04 g/L by using chloroform/methanol (2:1 v/v) mixture. The percentage yield on palm oil was 40.8%, giving 70.7 mm diameter in oil spreading technique. 2019-08 Thesis http://eprints.usm.my/49130/ http://eprints.usm.my/49130/1/NORAZURIN%20SYUHADA%20RUSLY%202019%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Rusly, Norazurin Syuhada Production And Characterization Of Biosurfactant Produced By Mangrove Streptomyces Sp. Pbd-410l
description	Biosurfactants are microbially produced surface-active compounds that reduce the surface tension between liquid phases of differing polarity. Presently, the best reported biosurfactant producers are Pseudomonas and Bacillus spp. Unfortunately, they are known opportunistic human pathogens, with some having antibiotic-resistance, causing grave concerns. Filamentous actinobacteria can be safer alternative biosurfactant producers, with no known human pathogenicity. The present study focused on the identification of biosurfactant-producing actinobacterium, characterization of the biosurfactant produced and the effect of fermentation parameters on biosurfactant production. A total of 60 actinobacterial isolates from mangrove sediments were screened for biosurfactant production using palm oil as the main carbon source. The presence of biosurfactant was confirmed using surface tension (ST) measurement, oil spreading technique (OST) and emulsification index (E24). Isolate PBD-410L was selected as the best biosurfactant producer based on the highest value for OST (68.0±1.7 mm) and the lowest surface tension measurement (39.7±0.6 dynes/cm). This isolate was characterized and tentatively identified as Streptomyces sp. PBD-410L, based on morphological characteristics and molecular identification. Crude biosurfactant extract was successfully recovered at a concentration of 11.04 g/L by using chloroform/methanol (2:1 v/v) mixture. The percentage yield on palm oil was 40.8%, giving 70.7 mm diameter in oil spreading technique.
format	Thesis
qualification_level	Master's degree
author	Rusly, Norazurin Syuhada
author_facet	Rusly, Norazurin Syuhada
author_sort	Rusly, Norazurin Syuhada
title	Production And Characterization Of Biosurfactant Produced By Mangrove Streptomyces Sp. Pbd-410l
title_short	Production And Characterization Of Biosurfactant Produced By Mangrove Streptomyces Sp. Pbd-410l
title_full	Production And Characterization Of Biosurfactant Produced By Mangrove Streptomyces Sp. Pbd-410l
title_fullStr	Production And Characterization Of Biosurfactant Produced By Mangrove Streptomyces Sp. Pbd-410l
title_full_unstemmed	Production And Characterization Of Biosurfactant Produced By Mangrove Streptomyces Sp. Pbd-410l
title_sort	production and characterization of biosurfactant produced by mangrove streptomyces sp. pbd-410l
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2019
url	http://eprints.usm.my/49130/1/NORAZURIN%20SYUHADA%20RUSLY%202019%20cut.pdf
_version_	1747821982457528320

Production And Characterization Of Biosurfactant Produced By Mangrove Streptomyces Sp. Pbd-410l

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