Comparison of three DNA extraction methods from raw cow's milk sample

Comparison of three DNA extraction methods from raw cow's milk sample

The progress and advancement in deoxyribonucleic acid (DNA) extraction has greatly evolved since it was initally performed in year 1986. DNA extraction has always be the first step required prior to any DNA analysis. In this study, raw cow’s milk has been subjected to three different extraction m...

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Main Author: Omar, Syahirah
Format: Thesis
Language:English
Published: 2020
Subjects:
R Medicine
Online Access:http://eprints.usm.my/49178/1/TESIS-SYAHIRAH%20OMAR%20%28P-SKM0023_19%29-24%20pages.pdf
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spelling my-usm-ep.491782021-05-19T04:31:44Z Comparison of three DNA extraction methods from raw cow's milk sample 2020-09 Omar, Syahirah R Medicine The progress and advancement in deoxyribonucleic acid (DNA) extraction has greatly evolved since it was initally performed in year 1986. DNA extraction has always be the first step required prior to any DNA analysis. In this study, raw cow’s milk has been subjected to three different extraction methods for determining the method that perform best in terms of DNA yield. Genomic DNA extracted from raw cow’s milk sample by the three methods was evaluated for purity and quantity by spectrophotometry and gel electrophoresis. On average, all three methods statistically showed no significant differences on final results of nucleic acid concentration, however, in terms of cost-effectiveness, conventional methods of phenol-chloroform and TENS method saved more compared to commercial kit. The results of this study showed that phenol-chloroform method had the best DNA yield based on the Nanodrop spectrophotometer measurement, followed by TENS method and commercial kit. 2020-09 Thesis http://eprints.usm.my/49178/ http://eprints.usm.my/49178/1/TESIS-SYAHIRAH%20OMAR%20%28P-SKM0023_19%29-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R Medicine
spellingShingle R Medicine
Omar, Syahirah
Comparison of three DNA extraction methods from raw cow's milk sample
description The progress and advancement in deoxyribonucleic acid (DNA) extraction has greatly evolved since it was initally performed in year 1986. DNA extraction has always be the first step required prior to any DNA analysis. In this study, raw cow’s milk has been subjected to three different extraction methods for determining the method that perform best in terms of DNA yield. Genomic DNA extracted from raw cow’s milk sample by the three methods was evaluated for purity and quantity by spectrophotometry and gel electrophoresis. On average, all three methods statistically showed no significant differences on final results of nucleic acid concentration, however, in terms of cost-effectiveness, conventional methods of phenol-chloroform and TENS method saved more compared to commercial kit. The results of this study showed that phenol-chloroform method had the best DNA yield based on the Nanodrop spectrophotometer measurement, followed by TENS method and commercial kit.
format Thesis
qualification_level Master's degree
author Omar, Syahirah
author_facet Omar, Syahirah
author_sort Omar, Syahirah
title Comparison of three DNA extraction methods from raw cow's milk sample
title_short Comparison of three DNA extraction methods from raw cow's milk sample
title_full Comparison of three DNA extraction methods from raw cow's milk sample
title_fullStr Comparison of three DNA extraction methods from raw cow's milk sample
title_full_unstemmed Comparison of three DNA extraction methods from raw cow's milk sample
title_sort comparison of three dna extraction methods from raw cow's milk sample
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Kesihatan
publishDate 2020
url http://eprints.usm.my/49178/1/TESIS-SYAHIRAH%20OMAR%20%28P-SKM0023_19%29-24%20pages.pdf
_version_ 1747821985392492544

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