Antimicrobial effect and microbial adherenceto maxillofacial prosthetic materials

Maxillofacial prostheses are used to restore the functional and anatomical defects of the maxillofacial region caused by trauma or tumour. It is beneficial to the patients if the materials used for the fabrication of the maxillofacial prostheses could demonstrate antimicrobial effects and resist...

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Main Author:	Rahman, Farhana
Format:	Thesis
Language:	English
Published:	2021
Subjects:	R Medicine
Online Access:	http://eprints.usm.my/49671/1/FARHANA%20RAHMAN-FINAL%20THESIS%20P-SGM000617%28R%29_24%20pages.pdf
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spelling	my-usm-ep.496712021-08-22T02:12:25Z Antimicrobial effect and microbial adherenceto maxillofacial prosthetic materials 2021-03 Rahman, Farhana R Medicine Maxillofacial prostheses are used to restore the functional and anatomical defects of the maxillofacial region caused by trauma or tumour. It is beneficial to the patients if the materials used for the fabrication of the maxillofacial prostheses could demonstrate antimicrobial effects and resist microbial adherence hence reducing the risk of infection. The objective of this study is to evaluate the antimicrobial effect, surface roughness and microbial adherence of the locally produced material namely modified polymethyl methacrylate (m-PMMA) against commercially produced polymethyl methacrylate (c-PMMA), silicone A-2000 and silicone A-2186 which are commonly used materials for maxillofacial prostheses. The microbial strains namely Staphylococcus aureus (S. aureus), Streptococcus mutans (S. mutans) and Candida albicans (C. albicans) were used in this study. Antimicrobial effect of maxillofacial prosthetic materials was determined by agar diffusion test, whilst microbial adherence was analysed using a direct colony-counting method and surface roughness of tested materials was determined using profilometer. Scanning electron microscopy (SEM) images were also used to examine the surface roughness and microbial adherence. One-way ANOVA was used to analyse surface roughness and Multivariate Analysis Of Variance (MANOVA) was used to analyse microbial adherence. For antimicrobial activity, it was observed that all tested materials did not inhibit the growth of all tested microbial strains. Surface roughness analysis showed significant difference (p<0.05) between PMMA and silicone elastomers. There was also significant difference in bacterial adherence on the tested materials in which significantly higher colony-forming unit (CFU) of S. aureus and S. mutans were observed on roughened surfaces namely silicone elastomers than that of PMMA (p>0.017). No significant difference was observed in the adherence of C. albicans between silicone elastomers and PMMA. It can be concluded that the presence of fillers in m-PMMA may not be adequate to promote the release of antimicrobial agents, however, m-PMMA showed less microbial adherence in comparison to other tested materials. The findings also demonstrate that surface roughness of the materials play an important role in microbial adherence. 2021-03 Thesis http://eprints.usm.my/49671/ http://eprints.usm.my/49671/1/FARHANA%20RAHMAN-FINAL%20THESIS%20P-SGM000617%28R%29_24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine
spellingShingle	R Medicine Rahman, Farhana Antimicrobial effect and microbial adherenceto maxillofacial prosthetic materials
description	Maxillofacial prostheses are used to restore the functional and anatomical defects of the maxillofacial region caused by trauma or tumour. It is beneficial to the patients if the materials used for the fabrication of the maxillofacial prostheses could demonstrate antimicrobial effects and resist microbial adherence hence reducing the risk of infection. The objective of this study is to evaluate the antimicrobial effect, surface roughness and microbial adherence of the locally produced material namely modified polymethyl methacrylate (m-PMMA) against commercially produced polymethyl methacrylate (c-PMMA), silicone A-2000 and silicone A-2186 which are commonly used materials for maxillofacial prostheses. The microbial strains namely Staphylococcus aureus (S. aureus), Streptococcus mutans (S. mutans) and Candida albicans (C. albicans) were used in this study. Antimicrobial effect of maxillofacial prosthetic materials was determined by agar diffusion test, whilst microbial adherence was analysed using a direct colony-counting method and surface roughness of tested materials was determined using profilometer. Scanning electron microscopy (SEM) images were also used to examine the surface roughness and microbial adherence. One-way ANOVA was used to analyse surface roughness and Multivariate Analysis Of Variance (MANOVA) was used to analyse microbial adherence. For antimicrobial activity, it was observed that all tested materials did not inhibit the growth of all tested microbial strains. Surface roughness analysis showed significant difference (p<0.05) between PMMA and silicone elastomers. There was also significant difference in bacterial adherence on the tested materials in which significantly higher colony-forming unit (CFU) of S. aureus and S. mutans were observed on roughened surfaces namely silicone elastomers than that of PMMA (p>0.017). No significant difference was observed in the adherence of C. albicans between silicone elastomers and PMMA. It can be concluded that the presence of fillers in m-PMMA may not be adequate to promote the release of antimicrobial agents, however, m-PMMA showed less microbial adherence in comparison to other tested materials. The findings also demonstrate that surface roughness of the materials play an important role in microbial adherence.
format	Thesis
qualification_level	Master's degree
author	Rahman, Farhana
author_facet	Rahman, Farhana
author_sort	Rahman, Farhana
title	Antimicrobial effect and microbial adherenceto maxillofacial prosthetic materials
title_short	Antimicrobial effect and microbial adherenceto maxillofacial prosthetic materials
title_full	Antimicrobial effect and microbial adherenceto maxillofacial prosthetic materials
title_fullStr	Antimicrobial effect and microbial adherenceto maxillofacial prosthetic materials
title_full_unstemmed	Antimicrobial effect and microbial adherenceto maxillofacial prosthetic materials
title_sort	antimicrobial effect and microbial adherenceto maxillofacial prosthetic materials
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2021
url	http://eprints.usm.my/49671/1/FARHANA%20RAHMAN-FINAL%20THESIS%20P-SGM000617%28R%29_24%20pages.pdf
_version_	1747822007713529856

Antimicrobial effect and microbial adherenceto maxillofacial prosthetic materials

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