The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used traditionally to treat various types of diseases especially in Thailand and Malaysia. Its anti-inflammatory and analgesic properties of its crude form are well documented. In this study, the cellular mechanism involved in th...
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| Format: | Thesis |
| Language: | English |
| Published: |
2014
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| Subjects: | |
| Online Access: | http://eprints.usm.my/50168/1/Zulkhurnain%20Bin%20Utar24.pdf |
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| Summary: | Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used
traditionally to treat various types of diseases especially in Thailand and Malaysia.
Its anti-inflammatory and analgesic properties of its crude form are well documented.
In this study, the cellular mechanism involved in the anti-inflammatory effects of
mitragynine, the major bioactive constituent, was investigated. The effects of
mitragynine on the mRNA and protein expression of COX-1 and COX-2 and the
production of prostaglandin E2 (PGE2) were investigated in LPS-treated RAW264.7
macrophage cells. Quantitative RT-PCR was used to assess the mRNA expression of
COX-1 and COX-2. Protein expression of COX-1 and COX-2 were assessed using
Western blot analysis and the level of PGE2 production was quantified using
Parameter™ PGE2 Assay (R&D Systems). |
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