The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells

Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used traditionally to treat various types of diseases especially in Thailand and Malaysia. Its anti-inflammatory and analgesic properties of its crude form are well documented. In this study, the cellular mechanism involved in th...

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Main Author: Utar, Zulkhurnain
Format: Thesis
Language:English
Published: 2014
Subjects:
Online Access:http://eprints.usm.my/50168/1/Zulkhurnain%20Bin%20Utar24.pdf
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spelling my-usm-ep.501682021-10-06T05:42:39Z The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells 2014-01 Utar, Zulkhurnain RK1-715 Dentistry Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used traditionally to treat various types of diseases especially in Thailand and Malaysia. Its anti-inflammatory and analgesic properties of its crude form are well documented. In this study, the cellular mechanism involved in the anti-inflammatory effects of mitragynine, the major bioactive constituent, was investigated. The effects of mitragynine on the mRNA and protein expression of COX-1 and COX-2 and the production of prostaglandin E2 (PGE2) were investigated in LPS-treated RAW264.7 macrophage cells. Quantitative RT-PCR was used to assess the mRNA expression of COX-1 and COX-2. Protein expression of COX-1 and COX-2 were assessed using Western blot analysis and the level of PGE2 production was quantified using Parameter™ PGE2 Assay (R&D Systems). 2014-01 Thesis http://eprints.usm.my/50168/ http://eprints.usm.my/50168/1/Zulkhurnain%20Bin%20Utar24.pdf application/pdf en public masters Universiti Sains Malaysia Institut Perubatan & Pergigian Termajui
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic RK1-715 Dentistry
spellingShingle RK1-715 Dentistry
Utar, Zulkhurnain
The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
description Mitragyna speciosa Korth (Rubiaceae) is one of the medicinal plants used traditionally to treat various types of diseases especially in Thailand and Malaysia. Its anti-inflammatory and analgesic properties of its crude form are well documented. In this study, the cellular mechanism involved in the anti-inflammatory effects of mitragynine, the major bioactive constituent, was investigated. The effects of mitragynine on the mRNA and protein expression of COX-1 and COX-2 and the production of prostaglandin E2 (PGE2) were investigated in LPS-treated RAW264.7 macrophage cells. Quantitative RT-PCR was used to assess the mRNA expression of COX-1 and COX-2. Protein expression of COX-1 and COX-2 were assessed using Western blot analysis and the level of PGE2 production was quantified using Parameter™ PGE2 Assay (R&D Systems).
format Thesis
qualification_level Master's degree
author Utar, Zulkhurnain
author_facet Utar, Zulkhurnain
author_sort Utar, Zulkhurnain
title The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
title_short The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
title_full The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
title_fullStr The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
title_full_unstemmed The Effects Of Mitragynine From Mitragyna Speciosa On The Mrna Expression Of Cox-1 And Cox-2 In Lipopolysaccharide-Stimulated Raw264.7 Macrophage Cells
title_sort effects of mitragynine from mitragyna speciosa on the mrna expression of cox-1 and cox-2 in lipopolysaccharide-stimulated raw264.7 macrophage cells
granting_institution Universiti Sains Malaysia
granting_department Institut Perubatan & Pergigian Termajui
publishDate 2014
url http://eprints.usm.my/50168/1/Zulkhurnain%20Bin%20Utar24.pdf
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