Determination of alpha hemoglobin stabilizing protein (AHSP) gene expression and oxidative stress parameters in HbE/beta-Thalassemia

Beta-Thalassaemia is the genetic disorders of haemoglobin synthesis characterized by reduced or absent β-globin chain synthesis thus lead to imbalance of tetramer. The Alpha Haemoglobin Stabilizing Protein (AHSP) acts as a molecular chaperone for α-globin by stabilizing free α-globin preventing it f...

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Main Author:	Yaacob, Nur Suraya Che
Format:	Thesis
Language:	English
Published:	2021
Subjects:	RK Dentistry
Online Access:	http://eprints.usm.my/50464/1/NUR%20SURAYA%20BINTI%20CHE%20YAACOB-%20FINAL%20THESIS%20P-UM000819%28R%29-24%20pages.pdf
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spelling	my-usm-ep.504642021-10-31T15:01:13Z Determination of alpha hemoglobin stabilizing protein (AHSP) gene expression and oxidative stress parameters in HbE/beta-Thalassemia 2021-07 Yaacob, Nur Suraya Che RK Dentistry Beta-Thalassaemia is the genetic disorders of haemoglobin synthesis characterized by reduced or absent β-globin chain synthesis thus lead to imbalance of tetramer. The Alpha Haemoglobin Stabilizing Protein (AHSP) acts as a molecular chaperone for α-globin by stabilizing free α-globin preventing it from precipitating and forming reactive oxygen species byproducts with subsequent oxidative damage and the formation of intracellular precipitates thus led to oxidative stress. Real time PCR and ELISA assay were used to determine the AHSP expression and oxidative stress parameters (SOD activity and MDA concentration) in blood sample respectively. Expression of AHSP significant in disease severity (p=0.001), transfusion dependency (p=0.033), molecular profile (p=0.035) and age (p=0.034), SOD activity significant in disease severity (p=0.005), transfusion dependency (p=0.001), serum ferritin level (p=0.005) and age (p=0.040), and MDA concentration significant in disease severity (p=0.003), transfusion dependency (p=0.001), splenectomy status (p=0.002), serum ferritin level (p=0.002) and age (p=0.015). We found that AHSP expression was significantly correlated to HbF (p=0.033) and frequency of blood transfusion per year (0.011) while SOD activity significantly correlated with age (p=0.033), HbF (p=0.009) and frequency of blood transfusion per year (p=0.004). On the other hand, MDA concentration was significantly correlated with age (p=0.008) and serum ferritin (p=0.022). In addition, correlation study was evaluated between AHSP expression with SOD activity and MDA concentration in which AHSP expression was significantly correlated with both parameters when p=0.002 and p=0.001 respectively. Thus, we concluded that AHSP could be a secondary compensatory mechanism in red blood cells to counterbalance the excess α-globin chains thus reduced the oxidative stress in HbE/Beta-Thalassaemia individuals. AHSP modifier and oxidative stress parameters give the future insight on its role in disease pathogenesis. 2021-07 Thesis http://eprints.usm.my/50464/ http://eprints.usm.my/50464/1/NUR%20SURAYA%20BINTI%20CHE%20YAACOB-%20FINAL%20THESIS%20P-UM000819%28R%29-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Pergigian
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RK Dentistry
spellingShingle	RK Dentistry Yaacob, Nur Suraya Che Determination of alpha hemoglobin stabilizing protein (AHSP) gene expression and oxidative stress parameters in HbE/beta-Thalassemia
description	Beta-Thalassaemia is the genetic disorders of haemoglobin synthesis characterized by reduced or absent β-globin chain synthesis thus lead to imbalance of tetramer. The Alpha Haemoglobin Stabilizing Protein (AHSP) acts as a molecular chaperone for α-globin by stabilizing free α-globin preventing it from precipitating and forming reactive oxygen species byproducts with subsequent oxidative damage and the formation of intracellular precipitates thus led to oxidative stress. Real time PCR and ELISA assay were used to determine the AHSP expression and oxidative stress parameters (SOD activity and MDA concentration) in blood sample respectively. Expression of AHSP significant in disease severity (p=0.001), transfusion dependency (p=0.033), molecular profile (p=0.035) and age (p=0.034), SOD activity significant in disease severity (p=0.005), transfusion dependency (p=0.001), serum ferritin level (p=0.005) and age (p=0.040), and MDA concentration significant in disease severity (p=0.003), transfusion dependency (p=0.001), splenectomy status (p=0.002), serum ferritin level (p=0.002) and age (p=0.015). We found that AHSP expression was significantly correlated to HbF (p=0.033) and frequency of blood transfusion per year (0.011) while SOD activity significantly correlated with age (p=0.033), HbF (p=0.009) and frequency of blood transfusion per year (p=0.004). On the other hand, MDA concentration was significantly correlated with age (p=0.008) and serum ferritin (p=0.022). In addition, correlation study was evaluated between AHSP expression with SOD activity and MDA concentration in which AHSP expression was significantly correlated with both parameters when p=0.002 and p=0.001 respectively. Thus, we concluded that AHSP could be a secondary compensatory mechanism in red blood cells to counterbalance the excess α-globin chains thus reduced the oxidative stress in HbE/Beta-Thalassaemia individuals. AHSP modifier and oxidative stress parameters give the future insight on its role in disease pathogenesis.
format	Thesis
qualification_level	Master's degree
author	Yaacob, Nur Suraya Che
author_facet	Yaacob, Nur Suraya Che
author_sort	Yaacob, Nur Suraya Che
title	Determination of alpha hemoglobin stabilizing protein (AHSP) gene expression and oxidative stress parameters in HbE/beta-Thalassemia
title_short	Determination of alpha hemoglobin stabilizing protein (AHSP) gene expression and oxidative stress parameters in HbE/beta-Thalassemia
title_full	Determination of alpha hemoglobin stabilizing protein (AHSP) gene expression and oxidative stress parameters in HbE/beta-Thalassemia
title_fullStr	Determination of alpha hemoglobin stabilizing protein (AHSP) gene expression and oxidative stress parameters in HbE/beta-Thalassemia
title_full_unstemmed	Determination of alpha hemoglobin stabilizing protein (AHSP) gene expression and oxidative stress parameters in HbE/beta-Thalassemia
title_sort	determination of alpha hemoglobin stabilizing protein (ahsp) gene expression and oxidative stress parameters in hbe/beta-thalassemia
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Pergigian
publishDate	2021
url	http://eprints.usm.my/50464/1/NUR%20SURAYA%20BINTI%20CHE%20YAACOB-%20FINAL%20THESIS%20P-UM000819%28R%29-24%20pages.pdf
_version_	1747822037857992704

Determination of alpha hemoglobin stabilizing protein (AHSP) gene expression and oxidative stress parameters in HbE/beta-Thalassemia

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