Synthesis And Application Of Molecularly Imprinted Solidphase Extraction For The Determination Of Terbutaline In Biological Matrices

A study was performed to evaluate the feasibility of applying MIPs as sorbent material in SPE for clean up of terbutaline from urine and plasma samples. The imprinted polymer was prepared by using methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker...

Full description

Saved in:

Bibliographic Details
Main Author:	Lim, Lay Lee
Format:	Thesis
Language:	English
Published:	2006
Subjects:	RS1-441 Pharmacy and materia medica
Online Access:	http://eprints.usm.my/51069/1/lim%20lay%20lee_hj.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.51069
record_format	uketd_dc
spelling	my-usm-ep.510692022-01-05T04:58:34Z Synthesis And Application Of Molecularly Imprinted Solidphase Extraction For The Determination Of Terbutaline In Biological Matrices 2006-12 Lim, Lay Lee RS1-441 Pharmacy and materia medica A study was performed to evaluate the feasibility of applying MIPs as sorbent material in SPE for clean up of terbutaline from urine and plasma samples. The imprinted polymer was prepared by using methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker and salbutamol, a closely structural analogue to the targeted analyte as template molecule. Porogen used was a mixture of acetonitrile-methanol (75: 25 % v/v). Free radical polymerisation was conducted at 50 °C for 24 hours. Molar ratio of template molecule: functional monomer: cross-linker applied was fixed at 1: 4: 20. The output was a monolith macroporous polymer which required grinding and sieving to obtain fine particles between 40 to 75 pm. Soxhlet extraction method was conducted to remove the imprint molecule in order produced simultaneously using the same procedure except in the absent of template molecule. A binding study of terbutaline in several solvents was performed to determine suitable solvent for loading, selective washing and elution steps. Selectivity test on the MIP against blank polymer demonstrated that both polymers exhibited different between both polymers were observed, indicating the presence of specific binding in imprinted polymer. In blank polymer, only weak non-specific interactions occurred. Cross-specificity studies showed the MIP also exhibited molecular recognition properties towards other structurally related compounds. Spiked urine and plasma samples were diluted in 100 mM ammonium acetate buffer pH 7 (ratio = 1:1 v/v) prior to direct loading (5 mL) into a cartridge filled with 50 mg of MIP. Thereafter, the column recognition properties towards terbutaline. Significant differences in elution curves to create the recognition sites. A blank/non-imprinted polymer was 2006-12 Thesis http://eprints.usm.my/51069/ http://eprints.usm.my/51069/1/lim%20lay%20lee_hj.pdf application/pdf en public masters Perpustakaan Hmzah Sendut Pusat Pengajian Sains Farmasi
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RS1-441 Pharmacy and materia medica
spellingShingle	RS1-441 Pharmacy and materia medica Lim, Lay Lee Synthesis And Application Of Molecularly Imprinted Solidphase Extraction For The Determination Of Terbutaline In Biological Matrices
description	A study was performed to evaluate the feasibility of applying MIPs as sorbent material in SPE for clean up of terbutaline from urine and plasma samples. The imprinted polymer was prepared by using methacrylic acid (MAA) as functional monomer, ethylene glycol dimethacrylate (EGDMA) as cross-linker and salbutamol, a closely structural analogue to the targeted analyte as template molecule. Porogen used was a mixture of acetonitrile-methanol (75: 25 % v/v). Free radical polymerisation was conducted at 50 °C for 24 hours. Molar ratio of template molecule: functional monomer: cross-linker applied was fixed at 1: 4: 20. The output was a monolith macroporous polymer which required grinding and sieving to obtain fine particles between 40 to 75 pm. Soxhlet extraction method was conducted to remove the imprint molecule in order produced simultaneously using the same procedure except in the absent of template molecule. A binding study of terbutaline in several solvents was performed to determine suitable solvent for loading, selective washing and elution steps. Selectivity test on the MIP against blank polymer demonstrated that both polymers exhibited different between both polymers were observed, indicating the presence of specific binding in imprinted polymer. In blank polymer, only weak non-specific interactions occurred. Cross-specificity studies showed the MIP also exhibited molecular recognition properties towards other structurally related compounds. Spiked urine and plasma samples were diluted in 100 mM ammonium acetate buffer pH 7 (ratio = 1:1 v/v) prior to direct loading (5 mL) into a cartridge filled with 50 mg of MIP. Thereafter, the column recognition properties towards terbutaline. Significant differences in elution curves to create the recognition sites. A blank/non-imprinted polymer was
format	Thesis
qualification_level	Master's degree
author	Lim, Lay Lee
author_facet	Lim, Lay Lee
author_sort	Lim, Lay Lee
title	Synthesis And Application Of Molecularly Imprinted Solidphase Extraction For The Determination Of Terbutaline In Biological Matrices
title_short	Synthesis And Application Of Molecularly Imprinted Solidphase Extraction For The Determination Of Terbutaline In Biological Matrices
title_full	Synthesis And Application Of Molecularly Imprinted Solidphase Extraction For The Determination Of Terbutaline In Biological Matrices
title_fullStr	Synthesis And Application Of Molecularly Imprinted Solidphase Extraction For The Determination Of Terbutaline In Biological Matrices
title_full_unstemmed	Synthesis And Application Of Molecularly Imprinted Solidphase Extraction For The Determination Of Terbutaline In Biological Matrices
title_sort	synthesis and application of molecularly imprinted solidphase extraction for the determination of terbutaline in biological matrices
granting_institution	Perpustakaan Hmzah Sendut
granting_department	Pusat Pengajian Sains Farmasi
publishDate	2006
url	http://eprints.usm.my/51069/1/lim%20lay%20lee_hj.pdf
_version_	1747822055475118080

Synthesis And Application Of Molecularly Imprinted Solidphase Extraction For The Determination Of Terbutaline In Biological Matrices

Similar Items