Development Of Working Electrode Modified By Iron Oxide Nanoparticles For Glucose Biosensor Applications

Glucose biosensor that is capable to provide wide linearity of detection, high sensitivity, and low limit of detection is important in clinical diagnosis. This has motivated the research into development of a better glucose biosensor. In this study, iron oxide nanoparticles (IONPs) were synthesiz...

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Main Author:	Mohamad Nor, Noorhashimah
Format:	Thesis
Language:	English
Published:	2019
Subjects:	T Technology
Online Access:	http://eprints.usm.my/51259/1/Development%20Of%20Working%20Electrode%20Modified%20By%20Iron%20Oxide%20Nanoparticles%20For%20Glucose%20Biosensor%20Applications.pdf
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spelling	my-usm-ep.512592022-01-20T08:02:29Z Development Of Working Electrode Modified By Iron Oxide Nanoparticles For Glucose Biosensor Applications 2019-02-01 Mohamad Nor, Noorhashimah T Technology TA401-492 Materials of engineering and construction. Mechanics of materials Glucose biosensor that is capable to provide wide linearity of detection, high sensitivity, and low limit of detection is important in clinical diagnosis. This has motivated the research into development of a better glucose biosensor. In this study, iron oxide nanoparticles (IONPs) were synthesized using the precipitation method and surface functionalized with varying citric acid (CA) concentration (0.10, 0.25, 0.50, and 0.70 g/ml) to produce stable colloidal IONPs in water. The effect of varying CA concentration on the crystallinity and morphology, of the IONPs–CA in water were studied using X-ray diffraction (XRD) and transmission electron microscope (TEM). From the XRD patterns, high crystallinity of spinel cubic lattice of maghemite (γ- Fe2O3) was obtained, while observation using transmission electron microscopy (TEM) showed particle size was in the range of 17–22 nm. The optimum CA concentration to functionalize IONPs–CA forming stable colloidal IONPs in water and exhibited excellent electrochemical performance was 0.25 g/ml. The stable colloidal IONPs–0.25 CA in water was then applied for fabrication of enzymatic and nonenzymatic glucose biosensor by modification of working electrode using drop casting method. In enzymatic glucose biosensor, the indium tin oxide (ITO) electrode and screen printed carbon electrode (SPCE) were modified with IONPs–0.25 CA, glucose oxidase (GOx) enzymes and Nafion layer. As for non-enzymatic glucose biosensor, the SPCE electrode was modified with IONPs–0.25 CA and Nafion layer. The optimization parameters of enzymatic and non-enzymatic glucose biosensors performance were conducted, such as effect of IONPs concentration, effect of GOx enzyme loading concentration, effect of working potential, effect of buffer solution pH and effect of operating temperature. The sensing performance of the developed enzymatic and non-enzymatic glucose biosensor exhibit excellent glucose detection performance with sensitivity of Nafion/GOx/IONPs–0.25 CA/ITO (941 μAmM-1cm-2 and limit of detection of 0.10 μM), Nafion/GOx/IONPs–0.25 CA/SPCE (164 μAmM- 1cm-2 and limit of detection of 14 μM), and 5Nafion/IONPs–0.25 CA/SPCE (2802 μAmM-1cm-2 and limit of detection 0.60 μM). The wide linearity of detection, high sensitivity and low limit of detection of enzymatic and non-enzymatic glucose biosensors were successfully developed based on modification of working electrode with IONPs–0.25 C 2019-02 Thesis http://eprints.usm.my/51259/ http://eprints.usm.my/51259/1/Development%20Of%20Working%20Electrode%20Modified%20By%20Iron%20Oxide%20Nanoparticles%20For%20Glucose%20Biosensor%20Applications.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Pengajian Kejuruteraan Bahan Sumber Mineral
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	T Technology T Technology
spellingShingle	T Technology T Technology Mohamad Nor, Noorhashimah Development Of Working Electrode Modified By Iron Oxide Nanoparticles For Glucose Biosensor Applications
description	Glucose biosensor that is capable to provide wide linearity of detection, high sensitivity, and low limit of detection is important in clinical diagnosis. This has motivated the research into development of a better glucose biosensor. In this study, iron oxide nanoparticles (IONPs) were synthesized using the precipitation method and surface functionalized with varying citric acid (CA) concentration (0.10, 0.25, 0.50, and 0.70 g/ml) to produce stable colloidal IONPs in water. The effect of varying CA concentration on the crystallinity and morphology, of the IONPs–CA in water were studied using X-ray diffraction (XRD) and transmission electron microscope (TEM). From the XRD patterns, high crystallinity of spinel cubic lattice of maghemite (γ- Fe2O3) was obtained, while observation using transmission electron microscopy (TEM) showed particle size was in the range of 17–22 nm. The optimum CA concentration to functionalize IONPs–CA forming stable colloidal IONPs in water and exhibited excellent electrochemical performance was 0.25 g/ml. The stable colloidal IONPs–0.25 CA in water was then applied for fabrication of enzymatic and nonenzymatic glucose biosensor by modification of working electrode using drop casting method. In enzymatic glucose biosensor, the indium tin oxide (ITO) electrode and screen printed carbon electrode (SPCE) were modified with IONPs–0.25 CA, glucose oxidase (GOx) enzymes and Nafion layer. As for non-enzymatic glucose biosensor, the SPCE electrode was modified with IONPs–0.25 CA and Nafion layer. The optimization parameters of enzymatic and non-enzymatic glucose biosensors performance were conducted, such as effect of IONPs concentration, effect of GOx enzyme loading concentration, effect of working potential, effect of buffer solution pH and effect of operating temperature. The sensing performance of the developed enzymatic and non-enzymatic glucose biosensor exhibit excellent glucose detection performance with sensitivity of Nafion/GOx/IONPs–0.25 CA/ITO (941 μAmM-1cm-2 and limit of detection of 0.10 μM), Nafion/GOx/IONPs–0.25 CA/SPCE (164 μAmM- 1cm-2 and limit of detection of 14 μM), and 5Nafion/IONPs–0.25 CA/SPCE (2802 μAmM-1cm-2 and limit of detection 0.60 μM). The wide linearity of detection, high sensitivity and low limit of detection of enzymatic and non-enzymatic glucose biosensors were successfully developed based on modification of working electrode with IONPs–0.25 C
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Mohamad Nor, Noorhashimah
author_facet	Mohamad Nor, Noorhashimah
author_sort	Mohamad Nor, Noorhashimah
title	Development Of Working Electrode Modified By Iron Oxide Nanoparticles For Glucose Biosensor Applications
title_short	Development Of Working Electrode Modified By Iron Oxide Nanoparticles For Glucose Biosensor Applications
title_full	Development Of Working Electrode Modified By Iron Oxide Nanoparticles For Glucose Biosensor Applications
title_fullStr	Development Of Working Electrode Modified By Iron Oxide Nanoparticles For Glucose Biosensor Applications
title_full_unstemmed	Development Of Working Electrode Modified By Iron Oxide Nanoparticles For Glucose Biosensor Applications
title_sort	development of working electrode modified by iron oxide nanoparticles for glucose biosensor applications
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Kejuruteraan Bahan Sumber Mineral
publishDate	2019
url	http://eprints.usm.my/51259/1/Development%20Of%20Working%20Electrode%20Modified%20By%20Iron%20Oxide%20Nanoparticles%20For%20Glucose%20Biosensor%20Applications.pdf
_version_	1747822066770378752

Development Of Working Electrode Modified By Iron Oxide Nanoparticles For Glucose Biosensor Applications

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