Hydrophobically Modified Pegylated Chitosan Derivative: Synthesis, Characterisation And Formulation Of Nanoparticles

Hydrophobic drugs encounter limitations such as poor solubility, vulnerable to degradation in biological environment and lack of selectivity towards targeted cells thus reduced the drug delivery and therapeutic efficacy. Drug-loaded nanoparticles (NPs) are widely studied for their potential to impro...

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Main Author:	Chong, Wai Mun
Format:	Thesis
Language:	English
Published:	2021
Subjects:	R5-920 Medicine (General)
Online Access:	http://eprints.usm.my/51837/1/CHONG%20WAI%20MUN.pdf
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id	my-usm-ep.51837
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spelling	my-usm-ep.518372022-03-08T06:43:47Z Hydrophobically Modified Pegylated Chitosan Derivative: Synthesis, Characterisation And Formulation Of Nanoparticles 2021-05 Chong, Wai Mun R5-920 Medicine (General) Hydrophobic drugs encounter limitations such as poor solubility, vulnerable to degradation in biological environment and lack of selectivity towards targeted cells thus reduced the drug delivery and therapeutic efficacy. Drug-loaded nanoparticles (NPs) are widely studied for their potential to improve the conventional medication by increasing the drug solubility and targeting of the diseased sites. Glycol chitosan (GC) is a versatile material as a nanocarrier as its structure can be modified, soluble in wide range of pHs, biocompatible and non-toxic. Itraconazole (ITZ) can be repurposed into an anticancer drug as it is capable of inhibiting growth of several types of cancer cells. The aim of this study was to develop palmitoylated GC polymer grafted with poly(ethylene) glycol (PEG) (PGC-PEG) and formulate the polymer with ITZ into stable NPs formulations. PGC-PEG was synthesised in a stepwise manner involving acid degradation, PEGylation and palmitoylation. The characterisation of several batches of PGC-PEG revealed polymer with 3.3% PEGylation and 59% palmitoylation with critical micelle concentration (CMC) value of 0.063 mg/mL as suitable to be incorporated with ITZ. The incorporation of PGC-PEG with ITZ (PGC-PEG-ITZ) produced homogenous positively charged formulations of polymeric micelles (PGC-PEG-ITZ-PM) and nanoemulsions (PGC-PEG-ITZ-NE). At drug to polymer ratio of 1:10, PGC-PEG-ITZ-NE showed higher drug entrapment (±80%) compared to that of 1:10 PGC-PEG-ITZ-PM (±40%). The average particle size of 1:10 PGC-PEG-ITZ-PM and PGC-PEG-ITZ-NE was 152-155 nm and 575-590 nm, respectively. 2021-05 Thesis http://eprints.usm.my/51837/ http://eprints.usm.my/51837/1/CHONG%20WAI%20MUN.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-920 Medicine (General)
spellingShingle	R5-920 Medicine (General) Chong, Wai Mun Hydrophobically Modified Pegylated Chitosan Derivative: Synthesis, Characterisation And Formulation Of Nanoparticles
description	Hydrophobic drugs encounter limitations such as poor solubility, vulnerable to degradation in biological environment and lack of selectivity towards targeted cells thus reduced the drug delivery and therapeutic efficacy. Drug-loaded nanoparticles (NPs) are widely studied for their potential to improve the conventional medication by increasing the drug solubility and targeting of the diseased sites. Glycol chitosan (GC) is a versatile material as a nanocarrier as its structure can be modified, soluble in wide range of pHs, biocompatible and non-toxic. Itraconazole (ITZ) can be repurposed into an anticancer drug as it is capable of inhibiting growth of several types of cancer cells. The aim of this study was to develop palmitoylated GC polymer grafted with poly(ethylene) glycol (PEG) (PGC-PEG) and formulate the polymer with ITZ into stable NPs formulations. PGC-PEG was synthesised in a stepwise manner involving acid degradation, PEGylation and palmitoylation. The characterisation of several batches of PGC-PEG revealed polymer with 3.3% PEGylation and 59% palmitoylation with critical micelle concentration (CMC) value of 0.063 mg/mL as suitable to be incorporated with ITZ. The incorporation of PGC-PEG with ITZ (PGC-PEG-ITZ) produced homogenous positively charged formulations of polymeric micelles (PGC-PEG-ITZ-PM) and nanoemulsions (PGC-PEG-ITZ-NE). At drug to polymer ratio of 1:10, PGC-PEG-ITZ-NE showed higher drug entrapment (±80%) compared to that of 1:10 PGC-PEG-ITZ-PM (±40%). The average particle size of 1:10 PGC-PEG-ITZ-PM and PGC-PEG-ITZ-NE was 152-155 nm and 575-590 nm, respectively.
format	Thesis
qualification_level	Master's degree
author	Chong, Wai Mun
author_facet	Chong, Wai Mun
author_sort	Chong, Wai Mun
title	Hydrophobically Modified Pegylated Chitosan Derivative: Synthesis, Characterisation And Formulation Of Nanoparticles
title_short	Hydrophobically Modified Pegylated Chitosan Derivative: Synthesis, Characterisation And Formulation Of Nanoparticles
title_full	Hydrophobically Modified Pegylated Chitosan Derivative: Synthesis, Characterisation And Formulation Of Nanoparticles
title_fullStr	Hydrophobically Modified Pegylated Chitosan Derivative: Synthesis, Characterisation And Formulation Of Nanoparticles
title_full_unstemmed	Hydrophobically Modified Pegylated Chitosan Derivative: Synthesis, Characterisation And Formulation Of Nanoparticles
title_sort	hydrophobically modified pegylated chitosan derivative: synthesis, characterisation and formulation of nanoparticles
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2021
url	http://eprints.usm.my/51837/1/CHONG%20WAI%20MUN.pdf
_version_	1747822104657526784

Hydrophobically Modified Pegylated Chitosan Derivative: Synthesis, Characterisation And Formulation Of Nanoparticles

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