Establishment Of Tissue Culture Planting Materials Of Gac (Momordica Cochinchinensis) Using Improved Light Emitting Diodes (Leds) Technology And Silver Nitrate Treatment

The Gac plant (Momordica cochinchinensis) of the cucurbitaceae family is native to the Southeast Asia region. The fruit has traditionally used as food and medicine due to their high levels of phytonutrients and antioxidants particularly beta-carotene and lycopene. M. cochinchinensis nodal explants u...

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Main Author: Chew, Hong Lim
Format: Thesis
Language:English
Published: 2021
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Online Access:http://eprints.usm.my/51838/1/CHEW%20HONG%20LIM.pdf
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spelling my-usm-ep.518382022-03-08T06:57:33Z Establishment Of Tissue Culture Planting Materials Of Gac (Momordica Cochinchinensis) Using Improved Light Emitting Diodes (Leds) Technology And Silver Nitrate Treatment 2021-03 Chew, Hong Lim QH1-278.5 Natural history (General) The Gac plant (Momordica cochinchinensis) of the cucurbitaceae family is native to the Southeast Asia region. The fruit has traditionally used as food and medicine due to their high levels of phytonutrients and antioxidants particularly beta-carotene and lycopene. M. cochinchinensis nodal explants used for the establishment of plant tissue culture system. The best plant growth regulator (PGR) for shoot regeneration in nodal segment explants of gac plant obtained by using semi-solid MS medium with 4mg/L BAP resulted an average of 9.2 shoots per explant and an average of 2.247 cm in length after 4 weeks. The best PGR for root induction for nodal segment explants of gac plant obtained by using semi-solid MS medium with 4mg/L IBA resulted an average of 13.2 roots per explant and 4.29cm in length after 4 weeks. After obtaining the optimum PGRs for the growth, gac nodal explants subjected with the treatment of various types of LED spectrums such as white (control), green, far-red, red, blue and purple [blue and red(1:1)] LEDs for duration of 4 weeks. Red LED spectrum displayed the most significant effect on shoot and root induction of gac explants. Explants under red illumination produced the highest average number of shoots which was 13.7 and an average of 2.193 cm in length. 2021-03 Thesis http://eprints.usm.my/51838/ http://eprints.usm.my/51838/1/CHEW%20HONG%20LIM.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1-278.5 Natural history (General)
spellingShingle QH1-278.5 Natural history (General)
Chew, Hong Lim
Establishment Of Tissue Culture Planting Materials Of Gac (Momordica Cochinchinensis) Using Improved Light Emitting Diodes (Leds) Technology And Silver Nitrate Treatment
description The Gac plant (Momordica cochinchinensis) of the cucurbitaceae family is native to the Southeast Asia region. The fruit has traditionally used as food and medicine due to their high levels of phytonutrients and antioxidants particularly beta-carotene and lycopene. M. cochinchinensis nodal explants used for the establishment of plant tissue culture system. The best plant growth regulator (PGR) for shoot regeneration in nodal segment explants of gac plant obtained by using semi-solid MS medium with 4mg/L BAP resulted an average of 9.2 shoots per explant and an average of 2.247 cm in length after 4 weeks. The best PGR for root induction for nodal segment explants of gac plant obtained by using semi-solid MS medium with 4mg/L IBA resulted an average of 13.2 roots per explant and 4.29cm in length after 4 weeks. After obtaining the optimum PGRs for the growth, gac nodal explants subjected with the treatment of various types of LED spectrums such as white (control), green, far-red, red, blue and purple [blue and red(1:1)] LEDs for duration of 4 weeks. Red LED spectrum displayed the most significant effect on shoot and root induction of gac explants. Explants under red illumination produced the highest average number of shoots which was 13.7 and an average of 2.193 cm in length.
format Thesis
qualification_level Master's degree
author Chew, Hong Lim
author_facet Chew, Hong Lim
author_sort Chew, Hong Lim
title Establishment Of Tissue Culture Planting Materials Of Gac (Momordica Cochinchinensis) Using Improved Light Emitting Diodes (Leds) Technology And Silver Nitrate Treatment
title_short Establishment Of Tissue Culture Planting Materials Of Gac (Momordica Cochinchinensis) Using Improved Light Emitting Diodes (Leds) Technology And Silver Nitrate Treatment
title_full Establishment Of Tissue Culture Planting Materials Of Gac (Momordica Cochinchinensis) Using Improved Light Emitting Diodes (Leds) Technology And Silver Nitrate Treatment
title_fullStr Establishment Of Tissue Culture Planting Materials Of Gac (Momordica Cochinchinensis) Using Improved Light Emitting Diodes (Leds) Technology And Silver Nitrate Treatment
title_full_unstemmed Establishment Of Tissue Culture Planting Materials Of Gac (Momordica Cochinchinensis) Using Improved Light Emitting Diodes (Leds) Technology And Silver Nitrate Treatment
title_sort establishment of tissue culture planting materials of gac (momordica cochinchinensis) using improved light emitting diodes (leds) technology and silver nitrate treatment
granting_institution Perpustakaan Hamzah Sendut
granting_department Pusat Pengajian Sains Kajihayat
publishDate 2021
url http://eprints.usm.my/51838/1/CHEW%20HONG%20LIM.pdf
_version_ 1747822104900796416