Effects Of Chrysin On The mRNA Expression Of Peroxisome Proliferator-Activated Receptor Alpha (PPARα) And Cyp Genes, Cell Cycle And Cell Migration In Colorectal Cancer Hct-116 Cells

Peroxisome proliferator-activated receptor-alpha (PPARα) is reported to regulate the transcription of cytochrome P450 genes in human liver and intestine, which accounts for 70-80% of the enzymes involved in drug metabolism. Chrysin, on the other hand, is a natural phytochemical flavonoid that may po...

Full description

Saved in:

Bibliographic Details
Main Author:	Khor, Chin Yin
Format:	Thesis
Language:	English
Published:	2020
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/51940/1/Pages%20from%20Effects%20Of%20Chrysin%20On%20The%20mRNA%20Expression%20Of%20Peroxisome%20Proliferator.pdf
Tags:	Add Tag No Tags, Be the first to tag this record!

id	my-usm-ep.51940
record_format	uketd_dc
spelling	my-usm-ep.519402022-03-17T06:57:01Z Effects Of Chrysin On The mRNA Expression Of Peroxisome Proliferator-Activated Receptor Alpha (PPARα) And Cyp Genes, Cell Cycle And Cell Migration In Colorectal Cancer Hct-116 Cells 2020-07 Khor, Chin Yin R735-854 Medical education. Medical schools. Research Peroxisome proliferator-activated receptor-alpha (PPARα) is reported to regulate the transcription of cytochrome P450 genes in human liver and intestine, which accounts for 70-80% of the enzymes involved in drug metabolism. Chrysin, on the other hand, is a natural phytochemical flavonoid that may possess a mild effect to be used as an anticancer agent. This study aimed to examine the effect of chrysin by investigating the mRNA expression level of PPARα and its related cellular mechanisms in treated HCT-116 cells, where camptothecin was used as a positive control The mRNA expressions of PPARα in HCT-116 cells treated with chrysin or a combination of chrysin and MK886 (PPARα inhibitor) were first determined using conventional PCR and then quantitatively analysed by Bioanalyzer. 2020-07 Thesis http://eprints.usm.my/51940/ http://eprints.usm.my/51940/1/Pages%20from%20Effects%20Of%20Chrysin%20On%20The%20mRNA%20Expression%20Of%20Peroxisome%20Proliferator.pdf application/pdf en public masters Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Khor, Chin Yin Effects Of Chrysin On The mRNA Expression Of Peroxisome Proliferator-Activated Receptor Alpha (PPARα) And Cyp Genes, Cell Cycle And Cell Migration In Colorectal Cancer Hct-116 Cells
description	Peroxisome proliferator-activated receptor-alpha (PPARα) is reported to regulate the transcription of cytochrome P450 genes in human liver and intestine, which accounts for 70-80% of the enzymes involved in drug metabolism. Chrysin, on the other hand, is a natural phytochemical flavonoid that may possess a mild effect to be used as an anticancer agent. This study aimed to examine the effect of chrysin by investigating the mRNA expression level of PPARα and its related cellular mechanisms in treated HCT-116 cells, where camptothecin was used as a positive control The mRNA expressions of PPARα in HCT-116 cells treated with chrysin or a combination of chrysin and MK886 (PPARα inhibitor) were first determined using conventional PCR and then quantitatively analysed by Bioanalyzer.
format	Thesis
qualification_level	Master's degree
author	Khor, Chin Yin
author_facet	Khor, Chin Yin
author_sort	Khor, Chin Yin
title	Effects Of Chrysin On The mRNA Expression Of Peroxisome Proliferator-Activated Receptor Alpha (PPARα) And Cyp Genes, Cell Cycle And Cell Migration In Colorectal Cancer Hct-116 Cells
title_short	Effects Of Chrysin On The mRNA Expression Of Peroxisome Proliferator-Activated Receptor Alpha (PPARα) And Cyp Genes, Cell Cycle And Cell Migration In Colorectal Cancer Hct-116 Cells
title_full	Effects Of Chrysin On The mRNA Expression Of Peroxisome Proliferator-Activated Receptor Alpha (PPARα) And Cyp Genes, Cell Cycle And Cell Migration In Colorectal Cancer Hct-116 Cells
title_fullStr	Effects Of Chrysin On The mRNA Expression Of Peroxisome Proliferator-Activated Receptor Alpha (PPARα) And Cyp Genes, Cell Cycle And Cell Migration In Colorectal Cancer Hct-116 Cells
title_full_unstemmed	Effects Of Chrysin On The mRNA Expression Of Peroxisome Proliferator-Activated Receptor Alpha (PPARα) And Cyp Genes, Cell Cycle And Cell Migration In Colorectal Cancer Hct-116 Cells
title_sort	effects of chrysin on the mrna expression of peroxisome proliferator-activated receptor alpha (pparα) and cyp genes, cell cycle and cell migration in colorectal cancer hct-116 cells
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2020
url	http://eprints.usm.my/51940/1/Pages%20from%20Effects%20Of%20Chrysin%20On%20The%20mRNA%20Expression%20Of%20Peroxisome%20Proliferator.pdf
_version_	1747822116377460736

Effects Of Chrysin On The mRNA Expression Of Peroxisome Proliferator-Activated Receptor Alpha (PPARα) And Cyp Genes, Cell Cycle And Cell Migration In Colorectal Cancer Hct-116 Cells

Similar Items