Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus

Avian flu is a serious disease that always give rise to devastated consequences in poultry industry. This disease is caused by influenza A virus bird’s strain. The genome of avian strain of influenza A virus (AIV) exhibits high mutation rate by means of antigenic drift and shift, leading to the emer...

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Main Author:	Yee, May Ye
Format:	Thesis
Language:	English
Published:	2020
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/52088/1/Pages%20from%20Development%20Of%20Multiplex%20Real-Time%20Reverse%20Transcription%20Pcr%20%28qRT-PCR%29.pdf
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spelling	my-usm-ep.520882022-05-26T04:48:05Z Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus 2020-11 Yee, May Ye R735-854 Medical education. Medical schools. Research Avian flu is a serious disease that always give rise to devastated consequences in poultry industry. This disease is caused by influenza A virus bird’s strain. The genome of avian strain of influenza A virus (AIV) exhibits high mutation rate by means of antigenic drift and shift, leading to the emergence of historic pandemics and epidemics. The first line of defence against avian influenza relies on rapid diagnosis. Current singleplex real-time RT-PCR (qRT-PCR) assay is not cost effective and internal control is not included. In this study, an in-house, two-stage multiplex qRT-PCR assay was developed for simultaneous detection and subtyping of H5, H7 and H9 subtype of AIV, the three most common subtypes isolated from poultry. 2020-11 Thesis http://eprints.usm.my/52088/ http://eprints.usm.my/52088/1/Pages%20from%20Development%20Of%20Multiplex%20Real-Time%20Reverse%20Transcription%20Pcr%20%28qRT-PCR%29.pdf application/pdf en public masters Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Yee, May Ye Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus
description	Avian flu is a serious disease that always give rise to devastated consequences in poultry industry. This disease is caused by influenza A virus bird’s strain. The genome of avian strain of influenza A virus (AIV) exhibits high mutation rate by means of antigenic drift and shift, leading to the emergence of historic pandemics and epidemics. The first line of defence against avian influenza relies on rapid diagnosis. Current singleplex real-time RT-PCR (qRT-PCR) assay is not cost effective and internal control is not included. In this study, an in-house, two-stage multiplex qRT-PCR assay was developed for simultaneous detection and subtyping of H5, H7 and H9 subtype of AIV, the three most common subtypes isolated from poultry.
format	Thesis
qualification_level	Master's degree
author	Yee, May Ye
author_facet	Yee, May Ye
author_sort	Yee, May Ye
title	Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus
title_short	Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus
title_full	Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus
title_fullStr	Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus
title_full_unstemmed	Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus
title_sort	development of multiplex real-time reverse transcription pcr (qrt-pcr) assay for detection and subtyping of avian influenza virus
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2020
url	http://eprints.usm.my/52088/1/Pages%20from%20Development%20Of%20Multiplex%20Real-Time%20Reverse%20Transcription%20Pcr%20%28qRT-PCR%29.pdf
_version_	1747822134394093568

Development Of Multiplex Real-Time Reverse Transcription Pcr (qRT-PCR) Assay For Detection And Subtyping Of Avian Influenza Virus

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