Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro

Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro

Although HAART effectively suppresses HIV-1 replication, its compromised effectiveness against non-B subtypes, the challenge of eliminating latent proviruses, life-long treatment, and viral resistance complicates the cure for HIV-1. CRISPR/Cas9, the latest genome editing tool, can overcome the limit...

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Main Author: Ravichantar, Nithya
Format: Thesis
Language:English
Published: 2020
Subjects:
R5-920 Medicine (General)
Online Access:http://eprints.usm.my/52124/1/Pages%20from%20Combinatorial%20CrisprCas9%20ForSuppression%20Of%20Latent%20Hiv-1%20Provirus%20In%20Vitro.pdf
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spelling my-usm-ep.521242022-03-31T07:08:35Z Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro 2020-11 Ravichantar, Nithya R5-920 Medicine (General) Although HAART effectively suppresses HIV-1 replication, its compromised effectiveness against non-B subtypes, the challenge of eliminating latent proviruses, life-long treatment, and viral resistance complicates the cure for HIV-1. CRISPR/Cas9, the latest genome editing tool, can overcome the limitations seen with HAART. By targeting the promoter of HIV-1 (LTR), CRISPR/Cas9 can disrupt the latent reservoirs. Here, we designed an improved CRISPR/Cas9 system, combinatorial CRISPR/Cas9 to concurrently knockdown multiple HIV-1 genes: structural (Pol and Gag), regulatory (Rev and Tat) and accessory genes (Vif). We investigated the efficacy of this tool as a therapy against different viral subtypes in a subset population . Our preliminary data, showed a huge decrease in viral load and we verified the safety of CRISPR/Cas9 in human cell lines. 2020-11 Thesis http://eprints.usm.my/52124/ http://eprints.usm.my/52124/1/Pages%20from%20Combinatorial%20CrisprCas9%20ForSuppression%20Of%20Latent%20Hiv-1%20Provirus%20In%20Vitro.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Institut Perubatan & Pergigian Termaju
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R5-920 Medicine (General)
spellingShingle R5-920 Medicine (General)
Ravichantar, Nithya
Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro
description Although HAART effectively suppresses HIV-1 replication, its compromised effectiveness against non-B subtypes, the challenge of eliminating latent proviruses, life-long treatment, and viral resistance complicates the cure for HIV-1. CRISPR/Cas9, the latest genome editing tool, can overcome the limitations seen with HAART. By targeting the promoter of HIV-1 (LTR), CRISPR/Cas9 can disrupt the latent reservoirs. Here, we designed an improved CRISPR/Cas9 system, combinatorial CRISPR/Cas9 to concurrently knockdown multiple HIV-1 genes: structural (Pol and Gag), regulatory (Rev and Tat) and accessory genes (Vif). We investigated the efficacy of this tool as a therapy against different viral subtypes in a subset population . Our preliminary data, showed a huge decrease in viral load and we verified the safety of CRISPR/Cas9 in human cell lines.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Ravichantar, Nithya
author_facet Ravichantar, Nithya
author_sort Ravichantar, Nithya
title Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro
title_short Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro
title_full Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro
title_fullStr Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro
title_full_unstemmed Combinatorial Crispr/Cas9 For Suppression Of Latent Hiv-1 Provirus In Vitro
title_sort combinatorial crispr/cas9 for suppression of latent hiv-1 provirus in vitro
granting_institution Universiti Sains Malaysia
granting_department Institut Perubatan & Pergigian Termaju
publishDate 2020
url http://eprints.usm.my/52124/1/Pages%20from%20Combinatorial%20CrisprCas9%20ForSuppression%20Of%20Latent%20Hiv-1%20Provirus%20In%20Vitro.pdf
_version_ 1747822136102223872

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