cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain)

cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain)

Dengue virus type-2 (DEN-2) is a member of the Flaviviridae family. DEN-2 has a single-stranded positive polarity ribonucleic acid (RNA) genome of 10,723 nucleotides in length that encodes for a single polyprotein precursor. In this work, viral RNA genome was extracted from Vero and C6/36 cells in...

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Main Author: Ong, Eng Keat
Format: Thesis
Language:English
Published: 2009
Subjects:
QH1 Natural history (General - Including nature conservation
geographical distribution)
Online Access:http://eprints.usm.my/52235/1/tesis%20DNA%20Clones%20cut.pdf
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spelling my-usm-ep.522352022-04-07T08:06:08Z cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain) 2009-04 Ong, Eng Keat QH1 Natural history (General - Including nature conservation, geographical distribution) Dengue virus type-2 (DEN-2) is a member of the Flaviviridae family. DEN-2 has a single-stranded positive polarity ribonucleic acid (RNA) genome of 10,723 nucleotides in length that encodes for a single polyprotein precursor. In this work, viral RNA genome was extracted from Vero and C6/36 cells infected with DEN-2 New Guinea C strain. The viral RNA genome of DEN-2 was reverse transcribed and amplified as seven overlapping fragments, covering the whole genome, by reverse transcription-polymerase chain reaction (RT-PCR). Each of these fragments was successfully cloned into a high copy number plasmid, pGEMR- T vector and transformed into Escherichia coli (E. coli) JM109 strain. The identity of each clone was verified using DNA sequencing. The seven overlapping fragments were successfully joined together in pGEMR-T vector and transformed into E. coli JM109 strain at 25°C with the presence of ampicillin (25 pg/ml) to produce a cDNA clone carrying the full-length genome of DEN-2. 2009-04 Thesis http://eprints.usm.my/52235/ http://eprints.usm.my/52235/1/tesis%20DNA%20Clones%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1 Natural history (General - Including nature conservation
geographical distribution)
spellingShingle QH1 Natural history (General - Including nature conservation
geographical distribution)
Ong, Eng Keat
cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain)
description Dengue virus type-2 (DEN-2) is a member of the Flaviviridae family. DEN-2 has a single-stranded positive polarity ribonucleic acid (RNA) genome of 10,723 nucleotides in length that encodes for a single polyprotein precursor. In this work, viral RNA genome was extracted from Vero and C6/36 cells infected with DEN-2 New Guinea C strain. The viral RNA genome of DEN-2 was reverse transcribed and amplified as seven overlapping fragments, covering the whole genome, by reverse transcription-polymerase chain reaction (RT-PCR). Each of these fragments was successfully cloned into a high copy number plasmid, pGEMR- T vector and transformed into Escherichia coli (E. coli) JM109 strain. The identity of each clone was verified using DNA sequencing. The seven overlapping fragments were successfully joined together in pGEMR-T vector and transformed into E. coli JM109 strain at 25°C with the presence of ampicillin (25 pg/ml) to produce a cDNA clone carrying the full-length genome of DEN-2.
format Thesis
qualification_level Master's degree
author Ong, Eng Keat
author_facet Ong, Eng Keat
author_sort Ong, Eng Keat
title cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain)
title_short cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain)
title_full cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain)
title_fullStr cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain)
title_full_unstemmed cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain)
title_sort cdna clones construction of dengue virus type-2 (new guinea c-strain)
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Sains Kajihayat
publishDate 2009
url http://eprints.usm.my/52235/1/tesis%20DNA%20Clones%20cut.pdf
_version_ 1747822151034994688

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