cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain)
Dengue virus type-2 (DEN-2) is a member of the Flaviviridae family. DEN-2 has a single-stranded positive polarity ribonucleic acid (RNA) genome of 10,723 nucleotides in length that encodes for a single polyprotein precursor. In this work, viral RNA genome was extracted from Vero and C6/36 cells in...
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my-usm-ep.522352022-04-07T08:06:08Z cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain) 2009-04 Ong, Eng Keat QH1 Natural history (General - Including nature conservation, geographical distribution) Dengue virus type-2 (DEN-2) is a member of the Flaviviridae family. DEN-2 has a single-stranded positive polarity ribonucleic acid (RNA) genome of 10,723 nucleotides in length that encodes for a single polyprotein precursor. In this work, viral RNA genome was extracted from Vero and C6/36 cells infected with DEN-2 New Guinea C strain. The viral RNA genome of DEN-2 was reverse transcribed and amplified as seven overlapping fragments, covering the whole genome, by reverse transcription-polymerase chain reaction (RT-PCR). Each of these fragments was successfully cloned into a high copy number plasmid, pGEMR- T vector and transformed into Escherichia coli (E. coli) JM109 strain. The identity of each clone was verified using DNA sequencing. The seven overlapping fragments were successfully joined together in pGEMR-T vector and transformed into E. coli JM109 strain at 25°C with the presence of ampicillin (25 pg/ml) to produce a cDNA clone carrying the full-length genome of DEN-2. 2009-04 Thesis http://eprints.usm.my/52235/ http://eprints.usm.my/52235/1/tesis%20DNA%20Clones%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat |
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Universiti Sains Malaysia |
collection |
USM Institutional Repository |
language |
English |
topic |
QH1 Natural history (General - Including nature conservation geographical distribution) |
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QH1 Natural history (General - Including nature conservation geographical distribution) Ong, Eng Keat cDNA Clones Construction Of Dengue Virus Type-2 (New Guinea C-Strain) |
description |
Dengue virus type-2 (DEN-2) is a member of the Flaviviridae family. DEN-2 has a single-stranded positive polarity ribonucleic acid (RNA) genome of
10,723 nucleotides in length that encodes for a single polyprotein precursor. In this
work, viral RNA genome was extracted from Vero and C6/36 cells infected with
DEN-2 New Guinea C strain. The viral RNA genome of DEN-2 was reverse
transcribed and amplified as seven overlapping fragments, covering the whole
genome, by reverse transcription-polymerase chain reaction (RT-PCR). Each of
these fragments was successfully cloned into a high copy number plasmid, pGEMR-
T vector and transformed into Escherichia coli (E. coli) JM109 strain. The identity
of each clone was verified using DNA sequencing. The seven overlapping fragments
were successfully joined together in pGEMR-T vector and transformed into E. coli
JM109 strain at 25°C with the presence of ampicillin (25 pg/ml) to produce a cDNA
clone carrying the full-length genome of DEN-2. |
format |
Thesis |
qualification_level |
Master's degree |
author |
Ong, Eng Keat |
author_facet |
Ong, Eng Keat |
author_sort |
Ong, Eng Keat |
title |
cDNA Clones Construction Of Dengue Virus Type-2
(New Guinea C-Strain) |
title_short |
cDNA Clones Construction Of Dengue Virus Type-2
(New Guinea C-Strain) |
title_full |
cDNA Clones Construction Of Dengue Virus Type-2
(New Guinea C-Strain) |
title_fullStr |
cDNA Clones Construction Of Dengue Virus Type-2
(New Guinea C-Strain) |
title_full_unstemmed |
cDNA Clones Construction Of Dengue Virus Type-2
(New Guinea C-Strain) |
title_sort |
cdna clones construction of dengue virus type-2
(new guinea c-strain) |
granting_institution |
Universiti Sains Malaysia |
granting_department |
Pusat Pengajian Sains Kajihayat |
publishDate |
2009 |
url |
http://eprints.usm.my/52235/1/tesis%20DNA%20Clones%20cut.pdf |
_version_ |
1747822151034994688 |