Molecular study of transfusion dependent thalassemia patients who attended pediatric day care Hospital Universiti Sains Malaysia, Kelantan from January 2006 to April 2006.

Thalassemia has emerged as one of the most common public health problems in Malaysia, particularly among Malaysian Chinese and Malays. This study aims to determine the spectrum of Thalassaemia gene mutations found in transfusion dependent Thalassaemia patients who attended Pediatric Daycare Unit,...

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Main Author:	Rozitah, Razman
Format:	Thesis
Language:	English
Published:	2008
Subjects:	R Medicine (General)
Online Access:	http://eprints.usm.my/52415/1/DR.%20ROZITAH%20BINTI%20RAZMAN%20-%2024%20pages.pdf
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id	my-usm-ep.52415
record_format	uketd_dc
spelling	my-usm-ep.524152022-04-26T04:31:48Z Molecular study of transfusion dependent thalassemia patients who attended pediatric day care Hospital Universiti Sains Malaysia, Kelantan from January 2006 to April 2006. 2008 Rozitah, Razman R Medicine (General) Thalassemia has emerged as one of the most common public health problems in Malaysia, particularly among Malaysian Chinese and Malays. This study aims to determine the spectrum of Thalassaemia gene mutations found in transfusion dependent Thalassaemia patients who attended Pediatric Daycare Unit, Hospital Universiti Sains Malaysia, Kelantan, Malaysia. The findings are important for establishing the prenatal diagnosis in our Human Genome Centre.This is a cross sectional study in which 38 transfusion dependent Beta Thalassaemia patients were screened for six different mutations previously shown to be prevalent in the Malaysian population. Sampel collection was started in January, 2006 till April, 2006. DNA was extracted from leucocytes collected from the peripheral vein, amplified by PCR and digested by six restriction enzymes for detection of mutations. The mutation were correlated with the clinical severity based on the following clinical parameters : age at presentation, pre-transfusion hemoglobin level, mean volume of blood transfusion per kilo body weight per year, spleen size, spleenectomy and growth failure were recorded in these patients to determine the severity of each group of thalassaemia type depicted by the mutation. For the statistical analysis, Kruskal-Wallis test and univariate analysis were used. 2008 Thesis http://eprints.usm.my/52415/ http://eprints.usm.my/52415/1/DR.%20ROZITAH%20BINTI%20RAZMAN%20-%2024%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Rozitah, Razman Molecular study of transfusion dependent thalassemia patients who attended pediatric day care Hospital Universiti Sains Malaysia, Kelantan from January 2006 to April 2006.
description	Thalassemia has emerged as one of the most common public health problems in Malaysia, particularly among Malaysian Chinese and Malays. This study aims to determine the spectrum of Thalassaemia gene mutations found in transfusion dependent Thalassaemia patients who attended Pediatric Daycare Unit, Hospital Universiti Sains Malaysia, Kelantan, Malaysia. The findings are important for establishing the prenatal diagnosis in our Human Genome Centre.This is a cross sectional study in which 38 transfusion dependent Beta Thalassaemia patients were screened for six different mutations previously shown to be prevalent in the Malaysian population. Sampel collection was started in January, 2006 till April, 2006. DNA was extracted from leucocytes collected from the peripheral vein, amplified by PCR and digested by six restriction enzymes for detection of mutations. The mutation were correlated with the clinical severity based on the following clinical parameters : age at presentation, pre-transfusion hemoglobin level, mean volume of blood transfusion per kilo body weight per year, spleen size, spleenectomy and growth failure were recorded in these patients to determine the severity of each group of thalassaemia type depicted by the mutation. For the statistical analysis, Kruskal-Wallis test and univariate analysis were used.
format	Thesis
qualification_level	Master's degree
author	Rozitah, Razman
author_facet	Rozitah, Razman
author_sort	Rozitah, Razman
title	Molecular study of transfusion dependent thalassemia patients who attended pediatric day care Hospital Universiti Sains Malaysia, Kelantan from January 2006 to April 2006.
title_short	Molecular study of transfusion dependent thalassemia patients who attended pediatric day care Hospital Universiti Sains Malaysia, Kelantan from January 2006 to April 2006.
title_full	Molecular study of transfusion dependent thalassemia patients who attended pediatric day care Hospital Universiti Sains Malaysia, Kelantan from January 2006 to April 2006.
title_fullStr	Molecular study of transfusion dependent thalassemia patients who attended pediatric day care Hospital Universiti Sains Malaysia, Kelantan from January 2006 to April 2006.
title_full_unstemmed	Molecular study of transfusion dependent thalassemia patients who attended pediatric day care Hospital Universiti Sains Malaysia, Kelantan from January 2006 to April 2006.
title_sort	molecular study of transfusion dependent thalassemia patients who attended pediatric day care hospital universiti sains malaysia, kelantan from january 2006 to april 2006.
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Perubatan
publishDate	2008
url	http://eprints.usm.my/52415/1/DR.%20ROZITAH%20BINTI%20RAZMAN%20-%2024%20pages.pdf
_version_	1747822176298336256

Molecular study of transfusion dependent thalassemia patients who attended pediatric day care Hospital Universiti Sains Malaysia, Kelantan from January 2006 to April 2006.

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