The role of tualang honey in modulating nociceptive responses in the thalamus of rapid eye movement (REM) sleep deprivation rat model

Rapid eye movement (REM) sleep deprivation has been postulated to contribute to the alteration of nociceptive responses; however, the causes and effects are poorly understood. The aim of this study was to investigate the effects of REM sleep deprivation on nociceptive behaviour score, histologica...

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Main Author:	Shafie, Anis Syahirah Mohd
Format:	Thesis
Language:	English
Published:	2022
Subjects:	R Medicine
Online Access:	http://eprints.usm.my/52556/1/ANIS%20SYAHIRAH%20BINTI%20MOHD%20SHAFIE-FINAL%20THESIS%20P-UM001020%28R%29%20PWD-24%20pages.pdf
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spelling	my-usm-ep.525562022-05-22T07:42:06Z The role of tualang honey in modulating nociceptive responses in the thalamus of rapid eye movement (REM) sleep deprivation rat model 2022-01 Shafie, Anis Syahirah Mohd R Medicine Rapid eye movement (REM) sleep deprivation has been postulated to contribute to the alteration of nociceptive responses; however, the causes and effects are poorly understood. The aim of this study was to investigate the effects of REM sleep deprivation on nociceptive behaviour score, histological changes, the level of oxidative stress parameters, and the level of NMDA R2 receptors in the thalamus of REM sleepdeprived rat model. Furthermore, this study also determined the effects of Tualang honey on the parameters investigated. Forty-eight Sprague-Dawley male rats were equally divided into four groups (n=12); free moving control (FMC), REM sleep deprivation for 72 hours (REMsd), REM sleep deprivation for 72 hours pretreated with Tualang honey (REMsdH) and tank control (TC). Tualang honey (1.2 g/kg body weight/ day) was given by oral gavage for 30 days prior to the REM sleep deprivation procedure. Following the experimental period, formalin test and nociceptive behaviour were conducted. The rats were sacrificed, and the thalamus was removed for histological examination and quantification of oxidative stress parameters and NMDA receptors levels. REMsdH group showed a significant decrease in nociceptive behaviour score compared to REMsd group (p<0.05). The number of Nissl-stained neurons was significantly higher in REMsdH compared to REMsd (p<0.05). Dark neurons were observed in the VPL region of the REMsd group but not in the REMsdH group. REMsdH showed a significant increase (p<0.001) in GSH, GR, SOD, and CAT levels but a significant decrease (p<0.001) in MDA and NMDA R2 levels in the thalamus compared to REMsd. In conclusion, the administration of Tualang honey has protective effects against the adverse effects of REM sleep deprivation. 2022-01 Thesis http://eprints.usm.my/52556/ http://eprints.usm.my/52556/1/ANIS%20SYAHIRAH%20BINTI%20MOHD%20SHAFIE-FINAL%20THESIS%20P-UM001020%28R%29%20PWD-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kesihatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine
spellingShingle	R Medicine Shafie, Anis Syahirah Mohd The role of tualang honey in modulating nociceptive responses in the thalamus of rapid eye movement (REM) sleep deprivation rat model
description	Rapid eye movement (REM) sleep deprivation has been postulated to contribute to the alteration of nociceptive responses; however, the causes and effects are poorly understood. The aim of this study was to investigate the effects of REM sleep deprivation on nociceptive behaviour score, histological changes, the level of oxidative stress parameters, and the level of NMDA R2 receptors in the thalamus of REM sleepdeprived rat model. Furthermore, this study also determined the effects of Tualang honey on the parameters investigated. Forty-eight Sprague-Dawley male rats were equally divided into four groups (n=12); free moving control (FMC), REM sleep deprivation for 72 hours (REMsd), REM sleep deprivation for 72 hours pretreated with Tualang honey (REMsdH) and tank control (TC). Tualang honey (1.2 g/kg body weight/ day) was given by oral gavage for 30 days prior to the REM sleep deprivation procedure. Following the experimental period, formalin test and nociceptive behaviour were conducted. The rats were sacrificed, and the thalamus was removed for histological examination and quantification of oxidative stress parameters and NMDA receptors levels. REMsdH group showed a significant decrease in nociceptive behaviour score compared to REMsd group (p<0.05). The number of Nissl-stained neurons was significantly higher in REMsdH compared to REMsd (p<0.05). Dark neurons were observed in the VPL region of the REMsd group but not in the REMsdH group. REMsdH showed a significant increase (p<0.001) in GSH, GR, SOD, and CAT levels but a significant decrease (p<0.001) in MDA and NMDA R2 levels in the thalamus compared to REMsd. In conclusion, the administration of Tualang honey has protective effects against the adverse effects of REM sleep deprivation.
format	Thesis
qualification_level	Master's degree
author	Shafie, Anis Syahirah Mohd
author_facet	Shafie, Anis Syahirah Mohd
author_sort	Shafie, Anis Syahirah Mohd
title	The role of tualang honey in modulating nociceptive responses in the thalamus of rapid eye movement (REM) sleep deprivation rat model
title_short	The role of tualang honey in modulating nociceptive responses in the thalamus of rapid eye movement (REM) sleep deprivation rat model
title_full	The role of tualang honey in modulating nociceptive responses in the thalamus of rapid eye movement (REM) sleep deprivation rat model
title_fullStr	The role of tualang honey in modulating nociceptive responses in the thalamus of rapid eye movement (REM) sleep deprivation rat model
title_full_unstemmed	The role of tualang honey in modulating nociceptive responses in the thalamus of rapid eye movement (REM) sleep deprivation rat model
title_sort	role of tualang honey in modulating nociceptive responses in the thalamus of rapid eye movement (rem) sleep deprivation rat model
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kesihatan
publishDate	2022
url	http://eprints.usm.my/52556/1/ANIS%20SYAHIRAH%20BINTI%20MOHD%20SHAFIE-FINAL%20THESIS%20P-UM001020%28R%29%20PWD-24%20pages.pdf
_version_	1747822190287388672

The role of tualang honey in modulating nociceptive responses in the thalamus of rapid eye movement (REM) sleep deprivation rat model

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