The Effect Of Phyllanthus Debilis Extract On Dna Methylation And Mirna Regulation In Ht-29 Colorectal Cancer Cell Line

Phyllanthus is one of the herbal plants that have been found to have anticancer property. Unfortunately, there is lack of study on the effect of the plant natural products on the epigenetics of cancer. Hence, this study was carried out to determine the effect of Phyllanthus debilis extract on DNA me...

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Main Author:	Zain, Siti Nur Dalila Mohd
Format:	Thesis
Language:	English
Published:	2021
Subjects:	RZ Other systems of medicine
Online Access:	http://eprints.usm.my/52730/1/SITI%20NUR%20DALILA%20BINTI%20MOHD%20ZAIN.pdf
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id	my-usm-ep.52730
record_format	uketd_dc
spelling	my-usm-ep.527302022-06-02T03:51:18Z The Effect Of Phyllanthus Debilis Extract On Dna Methylation And Mirna Regulation In Ht-29 Colorectal Cancer Cell Line 2021-03 Zain, Siti Nur Dalila Mohd RZ Other systems of medicine Phyllanthus is one of the herbal plants that have been found to have anticancer property. Unfortunately, there is lack of study on the effect of the plant natural products on the epigenetics of cancer. Hence, this study was carried out to determine the effect of Phyllanthus debilis extract on DNA methylation and miRNA regulation in HT-29 colorectal cancer cell line. The study was started by determining the most suitable Phyllanthus species to be used in the epigenetic regulation of HT-29 cell line. Three species of Phyllanthus named Phyllanthus urinaria, Phyllanthus niruri and Phyllanthus debilis were used. The phytochemicals of each Phyllanthus species were then extracted using 100% methanol and 100% water. The extracts were then tested for Total Phenolic Content (TPC) and Total Flavonoid content (TFC) and the antioxidant activities of the plant extracts using DPPH and ABTS assays. Then, the extracts were screened with HPLC to detect the presence of targeted compounds. The therapeutic index of each Phyllanthus extract was then measured on cancer cell lines (breast and colorectal cell lines) versus normal cell line (normal breast cell line). P. debilis methanolic extract showed highest therapeutic index when compared to other extracts. P. debilis methanolic extract was then used for further study to investigate its mechanisms in regulating DNA methylation, gene and miRNA expressions in HT-29 cell line that led to the cancer cell death. 2021-03 Thesis http://eprints.usm.my/52730/ http://eprints.usm.my/52730/1/SITI%20NUR%20DALILA%20BINTI%20MOHD%20ZAIN.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Institut Perubatan & Pergigian Termaju
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	RZ Other systems of medicine
spellingShingle	RZ Other systems of medicine Zain, Siti Nur Dalila Mohd The Effect Of Phyllanthus Debilis Extract On Dna Methylation And Mirna Regulation In Ht-29 Colorectal Cancer Cell Line
description	Phyllanthus is one of the herbal plants that have been found to have anticancer property. Unfortunately, there is lack of study on the effect of the plant natural products on the epigenetics of cancer. Hence, this study was carried out to determine the effect of Phyllanthus debilis extract on DNA methylation and miRNA regulation in HT-29 colorectal cancer cell line. The study was started by determining the most suitable Phyllanthus species to be used in the epigenetic regulation of HT-29 cell line. Three species of Phyllanthus named Phyllanthus urinaria, Phyllanthus niruri and Phyllanthus debilis were used. The phytochemicals of each Phyllanthus species were then extracted using 100% methanol and 100% water. The extracts were then tested for Total Phenolic Content (TPC) and Total Flavonoid content (TFC) and the antioxidant activities of the plant extracts using DPPH and ABTS assays. Then, the extracts were screened with HPLC to detect the presence of targeted compounds. The therapeutic index of each Phyllanthus extract was then measured on cancer cell lines (breast and colorectal cell lines) versus normal cell line (normal breast cell line). P. debilis methanolic extract showed highest therapeutic index when compared to other extracts. P. debilis methanolic extract was then used for further study to investigate its mechanisms in regulating DNA methylation, gene and miRNA expressions in HT-29 cell line that led to the cancer cell death.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Zain, Siti Nur Dalila Mohd
author_facet	Zain, Siti Nur Dalila Mohd
author_sort	Zain, Siti Nur Dalila Mohd
title	The Effect Of Phyllanthus Debilis Extract On Dna Methylation And Mirna Regulation In Ht-29 Colorectal Cancer Cell Line
title_short	The Effect Of Phyllanthus Debilis Extract On Dna Methylation And Mirna Regulation In Ht-29 Colorectal Cancer Cell Line
title_full	The Effect Of Phyllanthus Debilis Extract On Dna Methylation And Mirna Regulation In Ht-29 Colorectal Cancer Cell Line
title_fullStr	The Effect Of Phyllanthus Debilis Extract On Dna Methylation And Mirna Regulation In Ht-29 Colorectal Cancer Cell Line
title_full_unstemmed	The Effect Of Phyllanthus Debilis Extract On Dna Methylation And Mirna Regulation In Ht-29 Colorectal Cancer Cell Line
title_sort	effect of phyllanthus debilis extract on dna methylation and mirna regulation in ht-29 colorectal cancer cell line
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Institut Perubatan & Pergigian Termaju
publishDate	2021
url	http://eprints.usm.my/52730/1/SITI%20NUR%20DALILA%20BINTI%20MOHD%20ZAIN.pdf
_version_	1747822207914999808

The Effect Of Phyllanthus Debilis Extract On Dna Methylation And Mirna Regulation In Ht-29 Colorectal Cancer Cell Line

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