Partial Protease Characterization, In Vitro Protein Digestibility And Utilization Of Dietary Soybean In Giant Freshwater Prawn, Macrobrachium Rosenbergii (De Man)

Biochemical assays and electrophoretical observations using SDS-PAGE was conducted for partial identification and characterization of proteases in the digestive system of giant freshwater prawn (Macrobrachium rosenbergii). pH optima of the crude enzyme extracts of prawn showed 3 peaks in activity...

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Main Author:	Chisty, Md. Ay Az Hasan
Format:	Thesis
Language:	English
Published:	2005
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/52978/1/Md%20Ayaz%20Hasan%20Chisty.pdf
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id	my-usm-ep.52978
record_format	uketd_dc
spelling	my-usm-ep.529782022-06-21T07:52:09Z Partial Protease Characterization, In Vitro Protein Digestibility And Utilization Of Dietary Soybean In Giant Freshwater Prawn, Macrobrachium Rosenbergii (De Man) 2005-05 Chisty, Md. Ay Az Hasan QH1-278.5 Natural history (General) Biochemical assays and electrophoretical observations using SDS-PAGE was conducted for partial identification and characterization of proteases in the digestive system of giant freshwater prawn (Macrobrachium rosenbergii). pH optima of the crude enzyme extracts of prawn showed 3 peaks in activity at pH 3.0, 6.0 and 9.0. The acid proteinase and trypsin at pH 2.5 and 9.0 showed higher activity respectively at 3 and 5hrs after the last feeding. Presence of serine class proteinase was identified using PMSF that reduced 33% of the total proteolytic activity. Reduction in activity by 82% and 55% using specific inhibitors TLCK and TPCK confirmed the presence of trypsin and chymotrypsin respectively while EDTA reduced 34% activity resembles the presence of metallo-proteinase. Characterization of the alkaline proteinases by 12% SDS-PAGE, after incubation of extract with or without specific inhibitors, produced six active bands of 13 to 136 kDa molar mass. Two trypsin bands of 13 and 36 kDa, three chymotrypsin bands of 23, 47 and 73 kDa and one metallo-proteinase of 136 kDa were identified from zymogram. The results suggest that an acid protease responsible for the initial digestion of proteins and trypsin and chymotrypsin are the main enzymes responsible for the rest. 2005-05 Thesis http://eprints.usm.my/52978/ http://eprints.usm.my/52978/1/Md%20Ayaz%20Hasan%20Chisty.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Chisty, Md. Ay Az Hasan Partial Protease Characterization, In Vitro Protein Digestibility And Utilization Of Dietary Soybean In Giant Freshwater Prawn, Macrobrachium Rosenbergii (De Man)
description	Biochemical assays and electrophoretical observations using SDS-PAGE was conducted for partial identification and characterization of proteases in the digestive system of giant freshwater prawn (Macrobrachium rosenbergii). pH optima of the crude enzyme extracts of prawn showed 3 peaks in activity at pH 3.0, 6.0 and 9.0. The acid proteinase and trypsin at pH 2.5 and 9.0 showed higher activity respectively at 3 and 5hrs after the last feeding. Presence of serine class proteinase was identified using PMSF that reduced 33% of the total proteolytic activity. Reduction in activity by 82% and 55% using specific inhibitors TLCK and TPCK confirmed the presence of trypsin and chymotrypsin respectively while EDTA reduced 34% activity resembles the presence of metallo-proteinase. Characterization of the alkaline proteinases by 12% SDS-PAGE, after incubation of extract with or without specific inhibitors, produced six active bands of 13 to 136 kDa molar mass. Two trypsin bands of 13 and 36 kDa, three chymotrypsin bands of 23, 47 and 73 kDa and one metallo-proteinase of 136 kDa were identified from zymogram. The results suggest that an acid protease responsible for the initial digestion of proteins and trypsin and chymotrypsin are the main enzymes responsible for the rest.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Chisty, Md. Ay Az Hasan
author_facet	Chisty, Md. Ay Az Hasan
author_sort	Chisty, Md. Ay Az Hasan
title	Partial Protease Characterization, In Vitro Protein Digestibility And Utilization Of Dietary Soybean In Giant Freshwater Prawn, Macrobrachium Rosenbergii (De Man)
title_short	Partial Protease Characterization, In Vitro Protein Digestibility And Utilization Of Dietary Soybean In Giant Freshwater Prawn, Macrobrachium Rosenbergii (De Man)
title_full	Partial Protease Characterization, In Vitro Protein Digestibility And Utilization Of Dietary Soybean In Giant Freshwater Prawn, Macrobrachium Rosenbergii (De Man)
title_fullStr	Partial Protease Characterization, In Vitro Protein Digestibility And Utilization Of Dietary Soybean In Giant Freshwater Prawn, Macrobrachium Rosenbergii (De Man)
title_full_unstemmed	Partial Protease Characterization, In Vitro Protein Digestibility And Utilization Of Dietary Soybean In Giant Freshwater Prawn, Macrobrachium Rosenbergii (De Man)
title_sort	partial protease characterization, in vitro protein digestibility and utilization of dietary soybean in giant freshwater prawn, macrobrachium rosenbergii (de man)
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2005
url	http://eprints.usm.my/52978/1/Md%20Ayaz%20Hasan%20Chisty.pdf
_version_	1747822213813239808

Partial Protease Characterization, In Vitro Protein Digestibility And Utilization Of Dietary Soybean In Giant Freshwater Prawn, Macrobrachium Rosenbergii (De Man)

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