Expression Of PhaC1 And PhaC2 From Pseudomonas Sp. USM4-55 In Escherichia Coli Expression System
Polyhydroxyalkanoates (PHAs) are synthesized intracelullarly by a variety of microorganisms as reserve materials for carbon and energy source. PHA synthase (PhaC) is the key enzyme in the polymerization of PHAs. Pseudomonas sp. USM4-55 is a local isolate which is able to produce a blend of short...
Saved in:
| 主要作者: | |
|---|---|
| 格式: | Thesis |
| 語言: | English |
| 出版: |
2007
|
| 主題: | |
| 在線閱讀: | http://eprints.usm.my/53174/1/Tesis%20expression%20of%20phaC1%20cut.pdf |
| 標簽: |
添加標簽
沒有標簽, 成為第一個標記此記錄!
|
| id |
my-usm-ep.53174 |
|---|---|
| record_format |
uketd_dc |
| spelling |
my-usm-ep.531742022-06-29T02:52:05Z Expression Of PhaC1 And PhaC2 From Pseudomonas Sp. USM4-55 In Escherichia Coli Expression System 2007-09 Ghani, Balqis Ab. QH1 Natural history (General - Including nature conservation, geographical distribution) Polyhydroxyalkanoates (PHAs) are synthesized intracelullarly by a variety of microorganisms as reserve materials for carbon and energy source. PHA synthase (PhaC) is the key enzyme in the polymerization of PHAs. Pseudomonas sp. USM4-55 is a local isolate which is able to produce a blend of short chain length and medium chain length of polymer. Genes coding for PhaC (phaC1 and phaC2) from Pseudomonas sp. USM4-55 were amplified (each with a size of 1.7 kb) from the genomic DNA, cloned into pGEMR-T for sequencing and then expressed. For expression studies, pQE-30 was used as the expression vector under the control of PT5 promoter. These clones were named pQE-C1 and pQE-C2, and were induced with 0.01 mM of isopropyl-p-Dthiogalactoside (IPTG) at ODeoo of 0.6. 2007-09 Thesis http://eprints.usm.my/53174/ http://eprints.usm.my/53174/1/Tesis%20expression%20of%20phaC1%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
QH1 Natural history (General - Including nature conservation geographical distribution) |
| spellingShingle |
QH1 Natural history (General - Including nature conservation geographical distribution) Ghani, Balqis Ab. Expression Of PhaC1 And PhaC2 From Pseudomonas Sp. USM4-55 In Escherichia Coli Expression System |
| description |
Polyhydroxyalkanoates (PHAs) are synthesized intracelullarly by a variety of microorganisms as reserve materials for carbon and energy source.
PHA synthase (PhaC) is the key enzyme in the polymerization of PHAs.
Pseudomonas sp. USM4-55 is a local isolate which is able to produce a blend
of short chain length and medium chain length of polymer. Genes coding for
PhaC (phaC1 and phaC2) from Pseudomonas sp. USM4-55 were amplified
(each with a size of 1.7 kb) from the genomic DNA, cloned into pGEMR-T for
sequencing and then expressed. For expression studies, pQE-30 was used as
the expression vector under the control of PT5 promoter. These clones were
named pQE-C1 and pQE-C2, and were induced with 0.01 mM of isopropyl-p-Dthiogalactoside
(IPTG) at ODeoo of 0.6. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Ghani, Balqis Ab. |
| author_facet |
Ghani, Balqis Ab. |
| author_sort |
Ghani, Balqis Ab. |
| title |
Expression Of PhaC1 And PhaC2 From
Pseudomonas Sp. USM4-55 In
Escherichia Coli Expression System |
| title_short |
Expression Of PhaC1 And PhaC2 From
Pseudomonas Sp. USM4-55 In
Escherichia Coli Expression System |
| title_full |
Expression Of PhaC1 And PhaC2 From
Pseudomonas Sp. USM4-55 In
Escherichia Coli Expression System |
| title_fullStr |
Expression Of PhaC1 And PhaC2 From
Pseudomonas Sp. USM4-55 In
Escherichia Coli Expression System |
| title_full_unstemmed |
Expression Of PhaC1 And PhaC2 From
Pseudomonas Sp. USM4-55 In
Escherichia Coli Expression System |
| title_sort |
expression of phac1 and phac2 from
pseudomonas sp. usm4-55 in
escherichia coli expression system |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Kajihayat |
| publishDate |
2007 |
| url |
http://eprints.usm.my/53174/1/Tesis%20expression%20of%20phaC1%20cut.pdf |
| _version_ |
1747822226037538816 |