Characterisation And Crystallisation Of Deptor

The mechanistic target of rapamycin (mTOR) signalling pathway integrates wide varieties of nutrients to regulate important cellular processes related to cell growth. mTOR is a huge serine/threonine protein kinase and exists in two distinct complexes, mTORC1 and mTORC2. DEPTOR is an endogenous inhibi...

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Main Author:	Yeap, Kean Heng
Format:	Thesis
Language:	English
Published:	2021
Subjects:	QD Chemistry
Online Access:	http://eprints.usm.my/53203/1/YEAP%20KEAN%20HENG%20-%20TESIS.pdf%20cut.pdf
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id	my-usm-ep.53203
record_format	uketd_dc
spelling	my-usm-ep.532032022-06-30T09:17:57Z Characterisation And Crystallisation Of Deptor 2021-02 Yeap, Kean Heng QD Chemistry The mechanistic target of rapamycin (mTOR) signalling pathway integrates wide varieties of nutrients to regulate important cellular processes related to cell growth. mTOR is a huge serine/threonine protein kinase and exists in two distinct complexes, mTORC1 and mTORC2. DEPTOR is an endogenous inhibitor of mTOR and consists of two DEP domains in tandem arrangement and a PDZ domain. The Escherichia coli expression system was used for cloning and expression experiments. Here, besides the full-length protein, various truncated mutants were designed as well to gain insights into the characteristics and architectures of the protein. Four truncated mutants were constructed to represent the first DEP domain (DEP1), the second DEP domain (DEP2), both DEP domains (DEP12) and the PDZ domain. DEPTOR, DEP1, DEP2 and DEP12 were expressed as soluble proteins but PDZ was insoluble. 2021-02 Thesis http://eprints.usm.my/53203/ http://eprints.usm.my/53203/1/YEAP%20KEAN%20HENG%20-%20TESIS.pdf%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Biologi Kimia (Centre for Chemical Biology(CCB))
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QD Chemistry
spellingShingle	QD Chemistry Yeap, Kean Heng Characterisation And Crystallisation Of Deptor
description	The mechanistic target of rapamycin (mTOR) signalling pathway integrates wide varieties of nutrients to regulate important cellular processes related to cell growth. mTOR is a huge serine/threonine protein kinase and exists in two distinct complexes, mTORC1 and mTORC2. DEPTOR is an endogenous inhibitor of mTOR and consists of two DEP domains in tandem arrangement and a PDZ domain. The Escherichia coli expression system was used for cloning and expression experiments. Here, besides the full-length protein, various truncated mutants were designed as well to gain insights into the characteristics and architectures of the protein. Four truncated mutants were constructed to represent the first DEP domain (DEP1), the second DEP domain (DEP2), both DEP domains (DEP12) and the PDZ domain. DEPTOR, DEP1, DEP2 and DEP12 were expressed as soluble proteins but PDZ was insoluble.
format	Thesis
qualification_level	Master's degree
author	Yeap, Kean Heng
author_facet	Yeap, Kean Heng
author_sort	Yeap, Kean Heng
title	Characterisation And Crystallisation Of Deptor
title_short	Characterisation And Crystallisation Of Deptor
title_full	Characterisation And Crystallisation Of Deptor
title_fullStr	Characterisation And Crystallisation Of Deptor
title_full_unstemmed	Characterisation And Crystallisation Of Deptor
title_sort	characterisation and crystallisation of deptor
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Biologi Kimia (Centre for Chemical Biology(CCB))
publishDate	2021
url	http://eprints.usm.my/53203/1/YEAP%20KEAN%20HENG%20-%20TESIS.pdf%20cut.pdf
_version_	1747822228739719168

Characterisation And Crystallisation Of Deptor

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