Characterization Of Two Polyhydroxyalkanoate Synthase Genes (Phac) Of Cupriavidus Malaysiensis Usmaa1020t Isolated From Kulim Lake, Kedah, Malaysia

Cupriavidus malaysiensis strains USMAA1020T, USMAA2-4 and USMAHM13 were isolated from various natural environments of Peninsular Malaysia. USMAA1020T was isolated from sludge in Kulim Lake, Kedah. Meanwhile, USMAA2-4 was isolated from soil of Sungai Pinang, Penang whereas USMAHM13 was isolated from...

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Main Author:	Shafie, Nur Asilla Hani
Format:	Thesis
Language:	English
Published:	2021
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/53746/1/NUR%20ASILLA%20HANI%20BINTI%20SHAFIE%20-%20TESIS24.pdf
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id	my-usm-ep.53746
record_format	uketd_dc
spelling	my-usm-ep.537462022-07-31T17:19:02Z Characterization Of Two Polyhydroxyalkanoate Synthase Genes (Phac) Of Cupriavidus Malaysiensis Usmaa1020t Isolated From Kulim Lake, Kedah, Malaysia 2021-09 Shafie, Nur Asilla Hani QH1 Natural history (General - Including nature conservation, geographical distribution) Cupriavidus malaysiensis strains USMAA1020T, USMAA2-4 and USMAHM13 were isolated from various natural environments of Peninsular Malaysia. USMAA1020T was isolated from sludge in Kulim Lake, Kedah. Meanwhile, USMAA2-4 was isolated from soil of Sungai Pinang, Penang whereas USMAHM13 was isolated from sludge of a paddy field in Sg. Manik, Perak. The 16S rRNA gene sequence similarity between USMAA1020T, USMAA2-4, and USMAHM13 was 99%. In phylogenetic analysis, these strains formed a cluster in phylogenetic tree constructed when using both maximum-likelihood and neighbour joining method. Till date, there were many reported Cupriavidus strains which have been characterized and identified but only limited information available on the whole genome sequence of Cupriavidus strains especially strains that contain two functional polyhydroxyalkanoate synthase genes (phaC). In this study, we analyzed the whole genome sequence of these strains which are closely related to each other regardless the differences of isolating environments. The whole genome sequences were done using the PacBio RS II platform. The genomes sequenced (7.82 to 8.70 Mb) were comparatively larger than the type strain Cupriavidus necator H16T (7.42 Mb). Two polyhydroxyalkanoates synthase genes (phaC) were found in the annotated genomes of all the three strains. 2021-09 Thesis http://eprints.usm.my/53746/ http://eprints.usm.my/53746/1/NUR%20ASILLA%20HANI%20BINTI%20SHAFIE%20-%20TESIS24.pdf application/pdf en public masters Universiti Sains Malaysia. Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Shafie, Nur Asilla Hani Characterization Of Two Polyhydroxyalkanoate Synthase Genes (Phac) Of Cupriavidus Malaysiensis Usmaa1020t Isolated From Kulim Lake, Kedah, Malaysia
description	Cupriavidus malaysiensis strains USMAA1020T, USMAA2-4 and USMAHM13 were isolated from various natural environments of Peninsular Malaysia. USMAA1020T was isolated from sludge in Kulim Lake, Kedah. Meanwhile, USMAA2-4 was isolated from soil of Sungai Pinang, Penang whereas USMAHM13 was isolated from sludge of a paddy field in Sg. Manik, Perak. The 16S rRNA gene sequence similarity between USMAA1020T, USMAA2-4, and USMAHM13 was 99%. In phylogenetic analysis, these strains formed a cluster in phylogenetic tree constructed when using both maximum-likelihood and neighbour joining method. Till date, there were many reported Cupriavidus strains which have been characterized and identified but only limited information available on the whole genome sequence of Cupriavidus strains especially strains that contain two functional polyhydroxyalkanoate synthase genes (phaC). In this study, we analyzed the whole genome sequence of these strains which are closely related to each other regardless the differences of isolating environments. The whole genome sequences were done using the PacBio RS II platform. The genomes sequenced (7.82 to 8.70 Mb) were comparatively larger than the type strain Cupriavidus necator H16T (7.42 Mb). Two polyhydroxyalkanoates synthase genes (phaC) were found in the annotated genomes of all the three strains.
format	Thesis
qualification_level	Master's degree
author	Shafie, Nur Asilla Hani
author_facet	Shafie, Nur Asilla Hani
author_sort	Shafie, Nur Asilla Hani
title	Characterization Of Two Polyhydroxyalkanoate Synthase Genes (Phac) Of Cupriavidus Malaysiensis Usmaa1020t Isolated From Kulim Lake, Kedah, Malaysia
title_short	Characterization Of Two Polyhydroxyalkanoate Synthase Genes (Phac) Of Cupriavidus Malaysiensis Usmaa1020t Isolated From Kulim Lake, Kedah, Malaysia
title_full	Characterization Of Two Polyhydroxyalkanoate Synthase Genes (Phac) Of Cupriavidus Malaysiensis Usmaa1020t Isolated From Kulim Lake, Kedah, Malaysia
title_fullStr	Characterization Of Two Polyhydroxyalkanoate Synthase Genes (Phac) Of Cupriavidus Malaysiensis Usmaa1020t Isolated From Kulim Lake, Kedah, Malaysia
title_full_unstemmed	Characterization Of Two Polyhydroxyalkanoate Synthase Genes (Phac) Of Cupriavidus Malaysiensis Usmaa1020t Isolated From Kulim Lake, Kedah, Malaysia
title_sort	characterization of two polyhydroxyalkanoate synthase genes (phac) of cupriavidus malaysiensis usmaa1020t isolated from kulim lake, kedah, malaysia
granting_institution	Universiti Sains Malaysia.
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2021
url	http://eprints.usm.my/53746/1/NUR%20ASILLA%20HANI%20BINTI%20SHAFIE%20-%20TESIS24.pdf
_version_	1747822258493063168

Characterization Of Two Polyhydroxyalkanoate Synthase Genes (Phac) Of Cupriavidus Malaysiensis Usmaa1020t Isolated From Kulim Lake, Kedah, Malaysia

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