Molecular Cloning, Functional Characterization And Development Expression Of A New Elongase With Potential Role Of Long-Chain Polyunsaturated Fatty Acid Biosynthesis In A Teleost (Danio Rerio)

Elongase of very long-chained fatty acid (ELOVL) is very important in synthesising long-chained polyunsaturated fatty acid (LC-PUFA). At present, seven members of ELOVL family (ELOVL 1 – ELOVL 7) have been found in mammals. Recently, a putative new Elovl was found in teleost. In order to study and e...

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Main Author:	Goh, Pei Tian
Format:	Thesis
Language:	English
Published:	2020
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/53974/1/GOH%20PEI%20TIAN%20MSc.%202020.pdf
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id	my-usm-ep.53974
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spelling	my-usm-ep.539742022-08-10T08:19:33Z Molecular Cloning, Functional Characterization And Development Expression Of A New Elongase With Potential Role Of Long-Chain Polyunsaturated Fatty Acid Biosynthesis In A Teleost (Danio Rerio) 2020-02 Goh, Pei Tian QH1 Natural history (General - Including nature conservation, geographical distribution) Elongase of very long-chained fatty acid (ELOVL) is very important in synthesising long-chained polyunsaturated fatty acid (LC-PUFA). At present, seven members of ELOVL family (ELOVL 1 – ELOVL 7) have been found in mammals. Recently, a putative new Elovl was found in teleost. In order to study and examine the function of this new Elovl, zebrafish (Danio rerio) was chosen. Zebrafish is a viable lipid metabolism model with a complete LC-PUFA biosynthesis pathway and also a very good biological development model. An interest has arisen to determine the elongation capacity and also expression of this elongase of interest during development and in adult tissues. The full length of elongase of interest A (NM_001076593.1) and elongase of interest B (BC095712.1) genes were successfully cloned from the sequence available in National Center for Biotechnology Information (NCBI). A phylogenetic tree was constructed and revealed elongases of interest were grouped as a distinct group from the others elongases (Elovl1-7). Therefore they were named as elovl8a and elovl8b based on the result obtained from the phylogenetic tree. Other than that, Elovl2, Elovl4, Elovl5 and Elovl8 were found originated from the same ancestor and Elovl2, Elovl4 and Elov5 could be the result of gene expansion from Elovl8. Functional characterization of both elongases showed zebrafish Elovl8b converted monounsaturated fatty acid (MUFA), together with C18 and C20 polyunsaturated fatty acid (PUFA) substrates while Elovl8a lacked in these activities. 2020-02 Thesis http://eprints.usm.my/53974/ http://eprints.usm.my/53974/1/GOH%20PEI%20TIAN%20MSc.%202020.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Goh, Pei Tian Molecular Cloning, Functional Characterization And Development Expression Of A New Elongase With Potential Role Of Long-Chain Polyunsaturated Fatty Acid Biosynthesis In A Teleost (Danio Rerio)
description	Elongase of very long-chained fatty acid (ELOVL) is very important in synthesising long-chained polyunsaturated fatty acid (LC-PUFA). At present, seven members of ELOVL family (ELOVL 1 – ELOVL 7) have been found in mammals. Recently, a putative new Elovl was found in teleost. In order to study and examine the function of this new Elovl, zebrafish (Danio rerio) was chosen. Zebrafish is a viable lipid metabolism model with a complete LC-PUFA biosynthesis pathway and also a very good biological development model. An interest has arisen to determine the elongation capacity and also expression of this elongase of interest during development and in adult tissues. The full length of elongase of interest A (NM_001076593.1) and elongase of interest B (BC095712.1) genes were successfully cloned from the sequence available in National Center for Biotechnology Information (NCBI). A phylogenetic tree was constructed and revealed elongases of interest were grouped as a distinct group from the others elongases (Elovl1-7). Therefore they were named as elovl8a and elovl8b based on the result obtained from the phylogenetic tree. Other than that, Elovl2, Elovl4, Elovl5 and Elovl8 were found originated from the same ancestor and Elovl2, Elovl4 and Elov5 could be the result of gene expansion from Elovl8. Functional characterization of both elongases showed zebrafish Elovl8b converted monounsaturated fatty acid (MUFA), together with C18 and C20 polyunsaturated fatty acid (PUFA) substrates while Elovl8a lacked in these activities.
format	Thesis
qualification_level	Master's degree
author	Goh, Pei Tian
author_facet	Goh, Pei Tian
author_sort	Goh, Pei Tian
title	Molecular Cloning, Functional Characterization And Development Expression Of A New Elongase With Potential Role Of Long-Chain Polyunsaturated Fatty Acid Biosynthesis In A Teleost (Danio Rerio)
title_short	Molecular Cloning, Functional Characterization And Development Expression Of A New Elongase With Potential Role Of Long-Chain Polyunsaturated Fatty Acid Biosynthesis In A Teleost (Danio Rerio)
title_full	Molecular Cloning, Functional Characterization And Development Expression Of A New Elongase With Potential Role Of Long-Chain Polyunsaturated Fatty Acid Biosynthesis In A Teleost (Danio Rerio)
title_fullStr	Molecular Cloning, Functional Characterization And Development Expression Of A New Elongase With Potential Role Of Long-Chain Polyunsaturated Fatty Acid Biosynthesis In A Teleost (Danio Rerio)
title_full_unstemmed	Molecular Cloning, Functional Characterization And Development Expression Of A New Elongase With Potential Role Of Long-Chain Polyunsaturated Fatty Acid Biosynthesis In A Teleost (Danio Rerio)
title_sort	molecular cloning, functional characterization and development expression of a new elongase with potential role of long-chain polyunsaturated fatty acid biosynthesis in a teleost (danio rerio)
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2020
url	http://eprints.usm.my/53974/1/GOH%20PEI%20TIAN%20MSc.%202020.pdf
_version_	1747822267680686080

Molecular Cloning, Functional Characterization And Development Expression Of A New Elongase With Potential Role Of Long-Chain Polyunsaturated Fatty Acid Biosynthesis In A Teleost (Danio Rerio)

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