Performance Of Aqueous Impregnated Resin System For Purification Of Bacteriocin From Lactobacillus Bulgaricus Ftdc 1211

Conventional purification method of bacteriocins often requires laborious series of complicated procedures, high cost and low yield. Recent single purification step using aqueous two-phase system has long settling time, low interfacial tension and small density difference. To overcome this problem,...

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Main Author:	Aziz, Nur Fazrin Husna Abdul
Format:	Thesis
Language:	English
Published:	2020
Subjects:	T11.95-12.5 Industrial directories
Online Access:	http://eprints.usm.my/54733/1/NUR%20FAZRIN%20HUSNA%20BINTI%20ABDUL%20AZIZ%20-%20TESIS%20cut.pdf
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spelling	my-usm-ep.547332022-09-15T08:04:10Z Performance Of Aqueous Impregnated Resin System For Purification Of Bacteriocin From Lactobacillus Bulgaricus Ftdc 1211 2020-07 Aziz, Nur Fazrin Husna Abdul T11.95-12.5 Industrial directories Conventional purification method of bacteriocins often requires laborious series of complicated procedures, high cost and low yield. Recent single purification step using aqueous two-phase system has long settling time, low interfacial tension and small density difference. To overcome this problem, in our study, the aqueous impregnated resins system (AIRS) was proposed and developed to purify bacteriocin-like inhibitory substances produced by Lactobacillus bulgaricus FTDC 1211 which exhibited antimicrobial activity against Staphylococcus aureus in our study. In AIRS, hydrophobic polymer phase was impregnated into resins and hydrophilic aqueous phase acts as extraction solution to separate the bacteriocin in cell-free crude supernatant. The bacteriocin was then extracted in the polymer impregnated resins. Before conducting bacteriocin purification experiment, the performance of AIRS in extracting biological product was investigated using bovine serum albumin (BSA) as protein model. The impregnation stability of the hydrophobic polymer in three different types of resin (Amberlite XAD4, VitraPor glass pellets 4.0 mm and 8.0 mm) was investigated. Amberlite XAD4 resins showed the most stable impregnation of PEG with less than 1% (w/w) of leaching factor. A smooth surface on PEG impregnated Amberlite XAD4 was observed under scanning electron microscopy. 2020-07 Thesis http://eprints.usm.my/54733/ http://eprints.usm.my/54733/1/NUR%20FAZRIN%20HUSNA%20BINTI%20ABDUL%20AZIZ%20-%20TESIS%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Teknologi Industri
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	T11.95-12.5 Industrial directories
spellingShingle	T11.95-12.5 Industrial directories Aziz, Nur Fazrin Husna Abdul Performance Of Aqueous Impregnated Resin System For Purification Of Bacteriocin From Lactobacillus Bulgaricus Ftdc 1211
description	Conventional purification method of bacteriocins often requires laborious series of complicated procedures, high cost and low yield. Recent single purification step using aqueous two-phase system has long settling time, low interfacial tension and small density difference. To overcome this problem, in our study, the aqueous impregnated resins system (AIRS) was proposed and developed to purify bacteriocin-like inhibitory substances produced by Lactobacillus bulgaricus FTDC 1211 which exhibited antimicrobial activity against Staphylococcus aureus in our study. In AIRS, hydrophobic polymer phase was impregnated into resins and hydrophilic aqueous phase acts as extraction solution to separate the bacteriocin in cell-free crude supernatant. The bacteriocin was then extracted in the polymer impregnated resins. Before conducting bacteriocin purification experiment, the performance of AIRS in extracting biological product was investigated using bovine serum albumin (BSA) as protein model. The impregnation stability of the hydrophobic polymer in three different types of resin (Amberlite XAD4, VitraPor glass pellets 4.0 mm and 8.0 mm) was investigated. Amberlite XAD4 resins showed the most stable impregnation of PEG with less than 1% (w/w) of leaching factor. A smooth surface on PEG impregnated Amberlite XAD4 was observed under scanning electron microscopy.
format	Thesis
qualification_level	Master's degree
author	Aziz, Nur Fazrin Husna Abdul
author_facet	Aziz, Nur Fazrin Husna Abdul
author_sort	Aziz, Nur Fazrin Husna Abdul
title	Performance Of Aqueous Impregnated Resin System For Purification Of Bacteriocin From Lactobacillus Bulgaricus Ftdc 1211
title_short	Performance Of Aqueous Impregnated Resin System For Purification Of Bacteriocin From Lactobacillus Bulgaricus Ftdc 1211
title_full	Performance Of Aqueous Impregnated Resin System For Purification Of Bacteriocin From Lactobacillus Bulgaricus Ftdc 1211
title_fullStr	Performance Of Aqueous Impregnated Resin System For Purification Of Bacteriocin From Lactobacillus Bulgaricus Ftdc 1211
title_full_unstemmed	Performance Of Aqueous Impregnated Resin System For Purification Of Bacteriocin From Lactobacillus Bulgaricus Ftdc 1211
title_sort	performance of aqueous impregnated resin system for purification of bacteriocin from lactobacillus bulgaricus ftdc 1211
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Teknologi Industri
publishDate	2020
url	http://eprints.usm.my/54733/1/NUR%20FAZRIN%20HUSNA%20BINTI%20ABDUL%20AZIZ%20-%20TESIS%20cut.pdf
_version_	1747822291953123328

Performance Of Aqueous Impregnated Resin System For Purification Of Bacteriocin From Lactobacillus Bulgaricus Ftdc 1211

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