Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle

The abundance of xylose in agricultural waste represents an opportunity to turn it into energy or chemical feedstock. This study explores using E. coli JM109(DE3) harbouring pACYCxylE coding for a xylose transporter and pGEMCABcn carrying the PHA operon from C. necator to convert xylose into polyhyd...

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Main Author:	Arumugam, Deyvanai
Format:	Thesis
Language:	English
Published:	2021
Subjects:	QH1-278.5 Natural history (General)
Online Access:	http://eprints.usm.my/55086/1/DEYVANAI%20ARUMUGAM%20-%20Thesis.pdf
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id	my-usm-ep.55086
record_format	uketd_dc
spelling	my-usm-ep.550862022-10-03T08:07:31Z Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle 2021-06 Arumugam, Deyvanai QH1-278.5 Natural history (General) The abundance of xylose in agricultural waste represents an opportunity to turn it into energy or chemical feedstock. This study explores using E. coli JM109(DE3) harbouring pACYCxylE coding for a xylose transporter and pGEMCABcn carrying the PHA operon from C. necator to convert xylose into polyhydroxyalkanoate. Based on the criteria of fast growing, plasmid stability and ability to grow on minimal medium, strain JM109(DE3) was found to be the most suitable host for PHA production from xylose. The two plasmids were found to be stable after 10 serial overnight subcultures even without antibiotics selection. The strain was able to accumulate PHA up 62% CDW after 120 hours of incubation in mineral medium containing 1% xylose. Increasing xylose concentration up to 5% did not cause any further increase in PHA content, nor toxicity. Even at 1% xylose or glucose, the data suggests that the mineral medium was already limiting. When grown in 1% glucose, JM109(DE3) yield only 12% CDW PHA. Increasing glucose concentration to 5% leads to lower PHA content of only 6% CDW, implying glucose inhibition. In an experiment using a range of xylose:glucose ratio of 1:0.1 to 1:1, the PHA content fell from 58% to 32% CDW. In order to overcome glucose toxicity, six XylE mutants were tested for PHA production. 2021-06 Thesis http://eprints.usm.my/55086/ http://eprints.usm.my/55086/1/DEYVANAI%20ARUMUGAM%20-%20Thesis.pdf application/pdf en public masters Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1-278.5 Natural history (General)
spellingShingle	QH1-278.5 Natural history (General) Arumugam, Deyvanai Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle
description	The abundance of xylose in agricultural waste represents an opportunity to turn it into energy or chemical feedstock. This study explores using E. coli JM109(DE3) harbouring pACYCxylE coding for a xylose transporter and pGEMCABcn carrying the PHA operon from C. necator to convert xylose into polyhydroxyalkanoate. Based on the criteria of fast growing, plasmid stability and ability to grow on minimal medium, strain JM109(DE3) was found to be the most suitable host for PHA production from xylose. The two plasmids were found to be stable after 10 serial overnight subcultures even without antibiotics selection. The strain was able to accumulate PHA up 62% CDW after 120 hours of incubation in mineral medium containing 1% xylose. Increasing xylose concentration up to 5% did not cause any further increase in PHA content, nor toxicity. Even at 1% xylose or glucose, the data suggests that the mineral medium was already limiting. When grown in 1% glucose, JM109(DE3) yield only 12% CDW PHA. Increasing glucose concentration to 5% leads to lower PHA content of only 6% CDW, implying glucose inhibition. In an experiment using a range of xylose:glucose ratio of 1:0.1 to 1:1, the PHA content fell from 58% to 32% CDW. In order to overcome glucose toxicity, six XylE mutants were tested for PHA production.
format	Thesis
qualification_level	Master's degree
author	Arumugam, Deyvanai
author_facet	Arumugam, Deyvanai
author_sort	Arumugam, Deyvanai
title	Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle
title_short	Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle
title_full	Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle
title_fullStr	Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle
title_full_unstemmed	Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle
title_sort	production of polyhydroxyalkanoates from xylose by escherichia coli carrying wild type and mutant xyle
granting_institution	Perpustakaan Hamzah Sendut
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2021
url	http://eprints.usm.my/55086/1/DEYVANAI%20ARUMUGAM%20-%20Thesis.pdf
_version_	1747822311793229824

Production Of Polyhydroxyalkanoates From Xylose By Escherichia Coli Carrying Wild Type And Mutant Xyle

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