Micropropagation Of Meyer Lemon (Citrus X Meyeri)

Citrus x meyeri or Meyer lemon is the hybrid of true lemon and sweet orange (Citrus limon x Citrus sinensis) and native to China. The ingestion of Meyer lemon fruits is not only for its sweet flavour, but also for its high amounts of vitamin C and bioactive compounds that serve as antioxidant and an...

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Main Author:	Haradzi, Najwa Amalina
Format:	Thesis
Language:	English
Published:	2019
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/55572/1/NAJWA%20THESIS%20%28REAL%20FULL%29%20cut.pdf
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id	my-usm-ep.55572
record_format	uketd_dc
spelling	my-usm-ep.555722022-11-09T02:21:42Z Micropropagation Of Meyer Lemon (Citrus X Meyeri) 2019-12 Haradzi, Najwa Amalina QH1 Natural history (General - Including nature conservation, geographical distribution) Citrus x meyeri or Meyer lemon is the hybrid of true lemon and sweet orange (Citrus limon x Citrus sinensis) and native to China. The ingestion of Meyer lemon fruits is not only for its sweet flavour, but also for its high amounts of vitamin C and bioactive compounds that serve as antioxidant and anti-inflammatory agents. Current conventional techniques such as grafting and cutting used to propagate Meyer lemon in Malaysia were found to be less efficient for the production of plant stocks for commercial purposes. This study was aimed to establish in vitro protocol to mass propagate Meyer lemon through multiple shoot induction and to evaluate somatic embryogenesis formation. Surface sterilization was conducted on shoot tip and seed explants with agitation in 70% (v/v) of ethanol and different concentrations of Clorox® solution. Axenic shoot tips were inoculated in ½ strength Murashige and Skoog (MS) media with different cytokinin (BAP, Kinetin, Zeatin and Thidiazuron) and combinations of BAP with auxin, NAA and IAA. Root induction was performed in ½ strength MS and WPM media with different auxin, IAA and IBA. The well rooted shoots were acclimatized in black garden, red burnt and biochar soil mixture (2:1:1). Polymorphism analysis was carried out between cultivated and different subcultures of shoots using ISSR and SCoT markers. Light illumination, supplementation of single 2,4-D and combinations 0.5 mg/L 2,4-D with cytokinin (BAP, Kinetin and TDZ) were assessed for embryogenic callus formation using epicotyl segments from in vitro germinated seedlings. Similarly, light illumination and different cytokinin (BAP, Kinetin and TDZ) supplementation were also investigated for somatic embryos induction. 2019-12 Thesis http://eprints.usm.my/55572/ http://eprints.usm.my/55572/1/NAJWA%20THESIS%20%28REAL%20FULL%29%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Haradzi, Najwa Amalina Micropropagation Of Meyer Lemon (Citrus X Meyeri)
description	Citrus x meyeri or Meyer lemon is the hybrid of true lemon and sweet orange (Citrus limon x Citrus sinensis) and native to China. The ingestion of Meyer lemon fruits is not only for its sweet flavour, but also for its high amounts of vitamin C and bioactive compounds that serve as antioxidant and anti-inflammatory agents. Current conventional techniques such as grafting and cutting used to propagate Meyer lemon in Malaysia were found to be less efficient for the production of plant stocks for commercial purposes. This study was aimed to establish in vitro protocol to mass propagate Meyer lemon through multiple shoot induction and to evaluate somatic embryogenesis formation. Surface sterilization was conducted on shoot tip and seed explants with agitation in 70% (v/v) of ethanol and different concentrations of Clorox® solution. Axenic shoot tips were inoculated in ½ strength Murashige and Skoog (MS) media with different cytokinin (BAP, Kinetin, Zeatin and Thidiazuron) and combinations of BAP with auxin, NAA and IAA. Root induction was performed in ½ strength MS and WPM media with different auxin, IAA and IBA. The well rooted shoots were acclimatized in black garden, red burnt and biochar soil mixture (2:1:1). Polymorphism analysis was carried out between cultivated and different subcultures of shoots using ISSR and SCoT markers. Light illumination, supplementation of single 2,4-D and combinations 0.5 mg/L 2,4-D with cytokinin (BAP, Kinetin and TDZ) were assessed for embryogenic callus formation using epicotyl segments from in vitro germinated seedlings. Similarly, light illumination and different cytokinin (BAP, Kinetin and TDZ) supplementation were also investigated for somatic embryos induction.
format	Thesis
qualification_level	Master's degree
author	Haradzi, Najwa Amalina
author_facet	Haradzi, Najwa Amalina
author_sort	Haradzi, Najwa Amalina
title	Micropropagation Of Meyer Lemon (Citrus X Meyeri)
title_short	Micropropagation Of Meyer Lemon (Citrus X Meyeri)
title_full	Micropropagation Of Meyer Lemon (Citrus X Meyeri)
title_fullStr	Micropropagation Of Meyer Lemon (Citrus X Meyeri)
title_full_unstemmed	Micropropagation Of Meyer Lemon (Citrus X Meyeri)
title_sort	micropropagation of meyer lemon (citrus x meyeri)
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2019
url	http://eprints.usm.my/55572/1/NAJWA%20THESIS%20%28REAL%20FULL%29%20cut.pdf
_version_	1776101094768771072

Micropropagation Of Meyer Lemon (Citrus X Meyeri)

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