Development Of A High-Throughput Assay For Screening Plant Extracts That Can Extend The Chronological Life Span Of Yeast Saccharomyces Cerevisiae

Bioactive compounds are produced in different parts of a plant, and the compounds can be extracted as plant extracts (PEs). Some bioactive compounds have pharmacological properties such as antioxidant and lifespan-extending activities on organisms in vitro and in vivo. The budding yeast Saccharomyce...

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Main Author:	Mandy Kwong, Mun Yee
Format:	Thesis
Language:	English
Published:	2019
Subjects:	R735-854 Medical education Medical schools Research
Online Access:	http://eprints.usm.my/55599/1/24%20Pages%20from%20Mandy%20Kwong%20Mun%20Yee.pdf
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spelling	my-usm-ep.555992022-11-10T04:58:40Z Development Of A High-Throughput Assay For Screening Plant Extracts That Can Extend The Chronological Life Span Of Yeast Saccharomyces Cerevisiae 2019-11 Mandy Kwong, Mun Yee R735-854 Medical education. Medical schools. Research Bioactive compounds are produced in different parts of a plant, and the compounds can be extracted as plant extracts (PEs). Some bioactive compounds have pharmacological properties such as antioxidant and lifespan-extending activities on organisms in vitro and in vivo. The budding yeast Saccharomyces cerevisiae is a model organism for studying the mechanisms of ageing because yeast shares conserved pathways to higher eukaryotes. The chronological life span (CLS) of yeast is defined as the capacity of cells to remain viable in a non-dividing state. In this study, leaf extracts of of Manihot esculenta, Wodyetia bifurcata and Tabernaemontana divaricata were found to extend yeast CLS. A high-throughput 96-well based CLS assay was developed to enable quantitative screening of many PEs simultaneously to identify plant extracts with yeast CLS-extending activity. 2019-11 Thesis http://eprints.usm.my/55599/ http://eprints.usm.my/55599/1/24%20Pages%20from%20Mandy%20Kwong%20Mun%20Yee.pdf application/pdf en public masters Universiti Sains Malaysia Institut Penyelidikan Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R735-854 Medical education Medical schools Research
spellingShingle	R735-854 Medical education Medical schools Research Mandy Kwong, Mun Yee Development Of A High-Throughput Assay For Screening Plant Extracts That Can Extend The Chronological Life Span Of Yeast Saccharomyces Cerevisiae
description	Bioactive compounds are produced in different parts of a plant, and the compounds can be extracted as plant extracts (PEs). Some bioactive compounds have pharmacological properties such as antioxidant and lifespan-extending activities on organisms in vitro and in vivo. The budding yeast Saccharomyces cerevisiae is a model organism for studying the mechanisms of ageing because yeast shares conserved pathways to higher eukaryotes. The chronological life span (CLS) of yeast is defined as the capacity of cells to remain viable in a non-dividing state. In this study, leaf extracts of of Manihot esculenta, Wodyetia bifurcata and Tabernaemontana divaricata were found to extend yeast CLS. A high-throughput 96-well based CLS assay was developed to enable quantitative screening of many PEs simultaneously to identify plant extracts with yeast CLS-extending activity.
format	Thesis
qualification_level	Master's degree
author	Mandy Kwong, Mun Yee
author_facet	Mandy Kwong, Mun Yee
author_sort	Mandy Kwong, Mun Yee
title	Development Of A High-Throughput Assay For Screening Plant Extracts That Can Extend The Chronological Life Span Of Yeast Saccharomyces Cerevisiae
title_short	Development Of A High-Throughput Assay For Screening Plant Extracts That Can Extend The Chronological Life Span Of Yeast Saccharomyces Cerevisiae
title_full	Development Of A High-Throughput Assay For Screening Plant Extracts That Can Extend The Chronological Life Span Of Yeast Saccharomyces Cerevisiae
title_fullStr	Development Of A High-Throughput Assay For Screening Plant Extracts That Can Extend The Chronological Life Span Of Yeast Saccharomyces Cerevisiae
title_full_unstemmed	Development Of A High-Throughput Assay For Screening Plant Extracts That Can Extend The Chronological Life Span Of Yeast Saccharomyces Cerevisiae
title_sort	development of a high-throughput assay for screening plant extracts that can extend the chronological life span of yeast saccharomyces cerevisiae
granting_institution	Universiti Sains Malaysia
granting_department	Institut Penyelidikan Perubatan Molekul
publishDate	2019
url	http://eprints.usm.my/55599/1/24%20Pages%20from%20Mandy%20Kwong%20Mun%20Yee.pdf
_version_	1776101096922546176

Development Of A High-Throughput Assay For Screening Plant Extracts That Can Extend The Chronological Life Span Of Yeast Saccharomyces Cerevisiae

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