Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein

Toxocariasis is a neglected zoonotic disease with worldwide distribution and has been shown to be especially prevalent among children from socio-economically disadvantaged populations. The causative agents are Toxocara canis and Toxocara cati in which the definitive hosts are dogs and cats, respe...

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Main Author:	Tan Farrizam, Siti Naqiuyah
Format:	Thesis
Language:	English
Published:	2020
Subjects:	R5-130.5 General works
Online Access:	http://eprints.usm.my/55648/1/Siti%20Naqiuyah%20Tan%20Farrizam_Msc%20in%20Molecular%20Medicine_January%202020%20cut.pdf
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spelling	my-usm-ep.556482022-11-15T05:01:45Z Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein 2020-01 Tan Farrizam, Siti Naqiuyah R5-130.5 General works Toxocariasis is a neglected zoonotic disease with worldwide distribution and has been shown to be especially prevalent among children from socio-economically disadvantaged populations. The causative agents are Toxocara canis and Toxocara cati in which the definitive hosts are dogs and cats, respectively. Although it is acknowledged that human toxocariasis is caused by both T. canis and T. cati, however, the importance of T. cati as a zoonotic agent of the disease has been under-recognized since T. cati TES is rarely used for Toxocara serodiagnosis and T. canis TES antigens are generally thought to be able to detect infections by both species. Thus, it is highly likely that some cases of T. cati infection may be missed by tests using only T. canis antigen. Therefore, it is an urgent need for the development of diagnostic tools that use both T. cati and T. canis recombinant antigens to enable detection of infections caused by both species. Previously, a T. cati recombinant protein TES-120 was successfully produced and cloned into a pGEX-4T-1 expression vector which showed good diagnostic potential in western blot. However, the yield was low and unsuitable for rapid test development. In this study, the T. cati DNA insert was cloned into a pET32 expression vector and two short peptides tagged variants [five residues each of aspartic acid (D) and lysine(K)] were designed to increase the chances of obtaining a good yield of the soluble recombinant protein. All three variants namely TES-120 cati/pET32a, TES-120 5D cati/pET32a, and TES-120 5K cati/pET32a were expressed and purified. 2020-01 Thesis http://eprints.usm.my/55648/ http://eprints.usm.my/55648/1/Siti%20Naqiuyah%20Tan%20Farrizam_Msc%20in%20Molecular%20Medicine_January%202020%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Perubatan Molekul
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-130.5 General works
spellingShingle	R5-130.5 General works Tan Farrizam, Siti Naqiuyah Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein
description	Toxocariasis is a neglected zoonotic disease with worldwide distribution and has been shown to be especially prevalent among children from socio-economically disadvantaged populations. The causative agents are Toxocara canis and Toxocara cati in which the definitive hosts are dogs and cats, respectively. Although it is acknowledged that human toxocariasis is caused by both T. canis and T. cati, however, the importance of T. cati as a zoonotic agent of the disease has been under-recognized since T. cati TES is rarely used for Toxocara serodiagnosis and T. canis TES antigens are generally thought to be able to detect infections by both species. Thus, it is highly likely that some cases of T. cati infection may be missed by tests using only T. canis antigen. Therefore, it is an urgent need for the development of diagnostic tools that use both T. cati and T. canis recombinant antigens to enable detection of infections caused by both species. Previously, a T. cati recombinant protein TES-120 was successfully produced and cloned into a pGEX-4T-1 expression vector which showed good diagnostic potential in western blot. However, the yield was low and unsuitable for rapid test development. In this study, the T. cati DNA insert was cloned into a pET32 expression vector and two short peptides tagged variants [five residues each of aspartic acid (D) and lysine(K)] were designed to increase the chances of obtaining a good yield of the soluble recombinant protein. All three variants namely TES-120 cati/pET32a, TES-120 5D cati/pET32a, and TES-120 5K cati/pET32a were expressed and purified.
format	Thesis
qualification_level	Master's degree
author	Tan Farrizam, Siti Naqiuyah
author_facet	Tan Farrizam, Siti Naqiuyah
author_sort	Tan Farrizam, Siti Naqiuyah
title	Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein
title_short	Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein
title_full	Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein
title_fullStr	Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein
title_full_unstemmed	Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein
title_sort	development of a rapid test for toxocariasis using recombinant toxocara cati tes-120 protein
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian Perubatan Molekul
publishDate	2020
url	http://eprints.usm.my/55648/1/Siti%20Naqiuyah%20Tan%20Farrizam_Msc%20in%20Molecular%20Medicine_January%202020%20cut.pdf
_version_	1776101103903965184

Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein

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