Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein

Toxocariasis is a neglected zoonotic disease with worldwide distribution and has been shown to be especially prevalent among children from socio-economically disadvantaged populations. The causative agents are Toxocara canis and Toxocara cati in which the definitive hosts are dogs and cats, respe...

全面介紹

Saved in:
書目詳細資料
主要作者: Tan Farrizam, Siti Naqiuyah
格式: Thesis
語言:English
出版: 2020
主題:
在線閱讀:http://eprints.usm.my/55648/1/Siti%20Naqiuyah%20Tan%20Farrizam_Msc%20in%20Molecular%20Medicine_January%202020%20cut.pdf
標簽: 添加標簽
沒有標簽, 成為第一個標記此記錄!
id my-usm-ep.55648
record_format uketd_dc
spelling my-usm-ep.556482022-11-15T05:01:45Z Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein 2020-01 Tan Farrizam, Siti Naqiuyah R5-130.5 General works Toxocariasis is a neglected zoonotic disease with worldwide distribution and has been shown to be especially prevalent among children from socio-economically disadvantaged populations. The causative agents are Toxocara canis and Toxocara cati in which the definitive hosts are dogs and cats, respectively. Although it is acknowledged that human toxocariasis is caused by both T. canis and T. cati, however, the importance of T. cati as a zoonotic agent of the disease has been under-recognized since T. cati TES is rarely used for Toxocara serodiagnosis and T. canis TES antigens are generally thought to be able to detect infections by both species. Thus, it is highly likely that some cases of T. cati infection may be missed by tests using only T. canis antigen. Therefore, it is an urgent need for the development of diagnostic tools that use both T. cati and T. canis recombinant antigens to enable detection of infections caused by both species. Previously, a T. cati recombinant protein TES-120 was successfully produced and cloned into a pGEX-4T-1 expression vector which showed good diagnostic potential in western blot. However, the yield was low and unsuitable for rapid test development. In this study, the T. cati DNA insert was cloned into a pET32 expression vector and two short peptides tagged variants [five residues each of aspartic acid (D) and lysine(K)] were designed to increase the chances of obtaining a good yield of the soluble recombinant protein. All three variants namely TES-120 cati/pET32a, TES-120 5D cati/pET32a, and TES-120 5K cati/pET32a were expressed and purified. 2020-01 Thesis http://eprints.usm.my/55648/ http://eprints.usm.my/55648/1/Siti%20Naqiuyah%20Tan%20Farrizam_Msc%20in%20Molecular%20Medicine_January%202020%20cut.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Perubatan Molekul
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic R5-130.5 General works
spellingShingle R5-130.5 General works
Tan Farrizam, Siti Naqiuyah
Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein
description Toxocariasis is a neglected zoonotic disease with worldwide distribution and has been shown to be especially prevalent among children from socio-economically disadvantaged populations. The causative agents are Toxocara canis and Toxocara cati in which the definitive hosts are dogs and cats, respectively. Although it is acknowledged that human toxocariasis is caused by both T. canis and T. cati, however, the importance of T. cati as a zoonotic agent of the disease has been under-recognized since T. cati TES is rarely used for Toxocara serodiagnosis and T. canis TES antigens are generally thought to be able to detect infections by both species. Thus, it is highly likely that some cases of T. cati infection may be missed by tests using only T. canis antigen. Therefore, it is an urgent need for the development of diagnostic tools that use both T. cati and T. canis recombinant antigens to enable detection of infections caused by both species. Previously, a T. cati recombinant protein TES-120 was successfully produced and cloned into a pGEX-4T-1 expression vector which showed good diagnostic potential in western blot. However, the yield was low and unsuitable for rapid test development. In this study, the T. cati DNA insert was cloned into a pET32 expression vector and two short peptides tagged variants [five residues each of aspartic acid (D) and lysine(K)] were designed to increase the chances of obtaining a good yield of the soluble recombinant protein. All three variants namely TES-120 cati/pET32a, TES-120 5D cati/pET32a, and TES-120 5K cati/pET32a were expressed and purified.
format Thesis
qualification_level Master's degree
author Tan Farrizam, Siti Naqiuyah
author_facet Tan Farrizam, Siti Naqiuyah
author_sort Tan Farrizam, Siti Naqiuyah
title Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein
title_short Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein
title_full Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein
title_fullStr Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein
title_full_unstemmed Development Of A Rapid Test For Toxocariasis Using Recombinant Toxocara Cati Tes-120 Protein
title_sort development of a rapid test for toxocariasis using recombinant toxocara cati tes-120 protein
granting_institution Universiti Sains Malaysia
granting_department Pusat Pengajian Perubatan Molekul
publishDate 2020
url http://eprints.usm.my/55648/1/Siti%20Naqiuyah%20Tan%20Farrizam_Msc%20in%20Molecular%20Medicine_January%202020%20cut.pdf
_version_ 1776101103903965184