Comparative Proteomic, Glycoproteomic And Phosphoproteomic Analyses Of Breast Cancer Tissues

Breast cancer is the second-most leading cause of death among women worldwide. This is true in the Malaysian population as well; breast cancer is one of the most common types of cancer among women. This study was aimed to identify potential tumour proteins that are characteristic of breast carcinoge...

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Main Author:	Al-Wajeeh, Abdullah Saleh Saleh Abdullah
Format:	Thesis
Language:	English
Published:	2019
Subjects:	R5-130.5 General works
Online Access:	http://eprints.usm.my/55727/1/Abdullah%20thesis%20%28Final%20for%20examiners%29%20cut.pdf
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id	my-usm-ep.55727
record_format	uketd_dc
spelling	my-usm-ep.557272022-11-23T02:56:18Z Comparative Proteomic, Glycoproteomic And Phosphoproteomic Analyses Of Breast Cancer Tissues 2019-10 Al-Wajeeh, Abdullah Saleh Saleh Abdullah R5-130.5 General works Breast cancer is the second-most leading cause of death among women worldwide. This is true in the Malaysian population as well; breast cancer is one of the most common types of cancer among women. This study was aimed to identify potential tumour proteins that are characteristic of breast carcinogenesis. In order to detect the tumour-specific proteins, differential proteomic profiling was conducted using advanced proteomics techniques in healthy and tumour breast tissue samples. In this study, protein profiles of breast tumour and their adjacent normal tissues from Malaysian women were obtained and examined using GELFREE separation, LTQ-Orbitrap LC-MS/MS identification, and bioinformatic analyses. Findings of the current proteomic profile showed that among the proteins that were detected, several proteins were found with post-translation modifications (PTMs) such as phosphorylation and glycosylation. The analysis of proteins resulted in the identification of 137 proteins, out of that 21 proteins were significantly abundant in tumour tissues. In addition, 81 phosphoproteins were identified, out of that 12 proteins were found significantly higher in abundance in tumour tissues than compared to that their normal counterparts. Furthermore, the analysis identified a total of 73 glycoproteins, out of these 10 glycoproteins were recorded to be significantly abundant in tumour tissues. Generally, most of the identified proteins were found to be involved in physiological processes such as proliferation, cell survival, motility, invasion, angiogenesis, metastasis and stem cell self-renewal and cell structure signalling pathways. 2019-10 Thesis http://eprints.usm.my/55727/ http://eprints.usm.my/55727/1/Abdullah%20thesis%20%28Final%20for%20examiners%29%20cut.pdf application/pdf en public phd doctoral Universiti Sains Malaysia Pusat Penyelidikan Dadah dan Ubat-Ubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R5-130.5 General works
spellingShingle	R5-130.5 General works Al-Wajeeh, Abdullah Saleh Saleh Abdullah Comparative Proteomic, Glycoproteomic And Phosphoproteomic Analyses Of Breast Cancer Tissues
description	Breast cancer is the second-most leading cause of death among women worldwide. This is true in the Malaysian population as well; breast cancer is one of the most common types of cancer among women. This study was aimed to identify potential tumour proteins that are characteristic of breast carcinogenesis. In order to detect the tumour-specific proteins, differential proteomic profiling was conducted using advanced proteomics techniques in healthy and tumour breast tissue samples. In this study, protein profiles of breast tumour and their adjacent normal tissues from Malaysian women were obtained and examined using GELFREE separation, LTQ-Orbitrap LC-MS/MS identification, and bioinformatic analyses. Findings of the current proteomic profile showed that among the proteins that were detected, several proteins were found with post-translation modifications (PTMs) such as phosphorylation and glycosylation. The analysis of proteins resulted in the identification of 137 proteins, out of that 21 proteins were significantly abundant in tumour tissues. In addition, 81 phosphoproteins were identified, out of that 12 proteins were found significantly higher in abundance in tumour tissues than compared to that their normal counterparts. Furthermore, the analysis identified a total of 73 glycoproteins, out of these 10 glycoproteins were recorded to be significantly abundant in tumour tissues. Generally, most of the identified proteins were found to be involved in physiological processes such as proliferation, cell survival, motility, invasion, angiogenesis, metastasis and stem cell self-renewal and cell structure signalling pathways.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Al-Wajeeh, Abdullah Saleh Saleh Abdullah
author_facet	Al-Wajeeh, Abdullah Saleh Saleh Abdullah
author_sort	Al-Wajeeh, Abdullah Saleh Saleh Abdullah
title	Comparative Proteomic, Glycoproteomic And Phosphoproteomic Analyses Of Breast Cancer Tissues
title_short	Comparative Proteomic, Glycoproteomic And Phosphoproteomic Analyses Of Breast Cancer Tissues
title_full	Comparative Proteomic, Glycoproteomic And Phosphoproteomic Analyses Of Breast Cancer Tissues
title_fullStr	Comparative Proteomic, Glycoproteomic And Phosphoproteomic Analyses Of Breast Cancer Tissues
title_full_unstemmed	Comparative Proteomic, Glycoproteomic And Phosphoproteomic Analyses Of Breast Cancer Tissues
title_sort	comparative proteomic, glycoproteomic and phosphoproteomic analyses of breast cancer tissues
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Penyelidikan Dadah dan Ubat-Ubatan
publishDate	2019
url	http://eprints.usm.my/55727/1/Abdullah%20thesis%20%28Final%20for%20examiners%29%20cut.pdf
_version_	1776101109187739648

Comparative Proteomic, Glycoproteomic And Phosphoproteomic Analyses Of Breast Cancer Tissues

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