Characterisation Of Polyhydroxyalkanoate Synthesis

Polyhydroxyalkanoate (PHA) is a biodegradable and renewable thermoplastic that has potential to replace synthetic petroleum-based plastic due to their similar properties. Azotobacter vinelandii, a Gram-negative soil bacterium, is one of the microbes that can produce PHA. Many studies have been carri...

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Main Author:	Mok, Pei Shze
Format:	Thesis
Language:	English
Published:	2019
Subjects:	QH1 Natural history (General - Including nature conservation geographical distribution)
Online Access:	http://eprints.usm.my/56170/1/MOK%20PEI%20SHZE24.pdf
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id	my-usm-ep.56170
record_format	uketd_dc
spelling	my-usm-ep.561702022-12-31T13:02:55Z Characterisation Of Polyhydroxyalkanoate Synthesis 2019-11 Mok, Pei Shze QH1 Natural history (General - Including nature conservation, geographical distribution) Polyhydroxyalkanoate (PHA) is a biodegradable and renewable thermoplastic that has potential to replace synthetic petroleum-based plastic due to their similar properties. Azotobacter vinelandii, a Gram-negative soil bacterium, is one of the microbes that can produce PHA. Many studies have been carried out to increase the production or value of PHA. In this study, a wild type strain, A. vinelandii ATCC 12837, and a deletion mutant of A. vinelandii that without or lacks the S-layer, designated as ΔAvin_16040, were used to produce PHA. The PHA accumulation was evaluated under different parameters and compared with wild-type strain. Around 3-fold higher amount of PHA was obtainable by the mutant strain compared to the wild type strain and the absence of the S-layer surrounding the cell was believed to played important role on higher PHA production. Optimisation on PHA production by the mutant strain had shown increment of 6 % and 39.5 % for cell dry weight and PHA concentration, respectively. The properties of poly(3-hydroxybutyrate), [P(3HB)] between two A. vinelandii strains were similar in terms of molecular weight, thermal and tensile properties. Besides, this bacterium is known to produce PHA containing 3-hydroxybutyrate (3HB) or mixture of 3HB and 3-hydroxyvalerate (3HV). Interestingly, in this study, 4-hydroxybutyrate (4HB) monomer was discovered to be incorporated for formation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] by both the wild type and mutant strains when structurally-related carbon source was provided. 2019-11 Thesis http://eprints.usm.my/56170/ http://eprints.usm.my/56170/1/MOK%20PEI%20SHZE24.pdf application/pdf en public phd doctoral Universiti Sains Malaysia. Pusat Pengajian Sains Kajihayat
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	QH1 Natural history (General - Including nature conservation geographical distribution)
spellingShingle	QH1 Natural history (General - Including nature conservation geographical distribution) Mok, Pei Shze Characterisation Of Polyhydroxyalkanoate Synthesis
description	Polyhydroxyalkanoate (PHA) is a biodegradable and renewable thermoplastic that has potential to replace synthetic petroleum-based plastic due to their similar properties. Azotobacter vinelandii, a Gram-negative soil bacterium, is one of the microbes that can produce PHA. Many studies have been carried out to increase the production or value of PHA. In this study, a wild type strain, A. vinelandii ATCC 12837, and a deletion mutant of A. vinelandii that without or lacks the S-layer, designated as ΔAvin_16040, were used to produce PHA. The PHA accumulation was evaluated under different parameters and compared with wild-type strain. Around 3-fold higher amount of PHA was obtainable by the mutant strain compared to the wild type strain and the absence of the S-layer surrounding the cell was believed to played important role on higher PHA production. Optimisation on PHA production by the mutant strain had shown increment of 6 % and 39.5 % for cell dry weight and PHA concentration, respectively. The properties of poly(3-hydroxybutyrate), [P(3HB)] between two A. vinelandii strains were similar in terms of molecular weight, thermal and tensile properties. Besides, this bacterium is known to produce PHA containing 3-hydroxybutyrate (3HB) or mixture of 3HB and 3-hydroxyvalerate (3HV). Interestingly, in this study, 4-hydroxybutyrate (4HB) monomer was discovered to be incorporated for formation of poly(3-hydroxybutyrate-co-4-hydroxybutyrate) [P(3HB-co-4HB)] by both the wild type and mutant strains when structurally-related carbon source was provided.
format	Thesis
qualification_name	Doctor of Philosophy (PhD.)
qualification_level	Doctorate
author	Mok, Pei Shze
author_facet	Mok, Pei Shze
author_sort	Mok, Pei Shze
title	Characterisation Of Polyhydroxyalkanoate Synthesis
title_short	Characterisation Of Polyhydroxyalkanoate Synthesis
title_full	Characterisation Of Polyhydroxyalkanoate Synthesis
title_fullStr	Characterisation Of Polyhydroxyalkanoate Synthesis
title_full_unstemmed	Characterisation Of Polyhydroxyalkanoate Synthesis
title_sort	characterisation of polyhydroxyalkanoate synthesis
granting_institution	Universiti Sains Malaysia.
granting_department	Pusat Pengajian Sains Kajihayat
publishDate	2019
url	http://eprints.usm.my/56170/1/MOK%20PEI%20SHZE24.pdf
_version_	1776101143455203328

Characterisation Of Polyhydroxyalkanoate Synthesis

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