Evaluation of the wound healing in full thickness skin autograft with different doses of topical stichopus spj extract in sprague dawley rats

Gamat refers to a collection of sea cucumbers most commonly consumed as traditional remedies by Malaysians. With its known healing properties to date, the effect of topical gamat extract could be beneficial in the healing process of the skin grafts and the effective dose could be determined. Obje...

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Main Author:	Sulong, Husnaida Abdul Manan
Format:	Thesis
Language:	English
Published:	2010
Subjects:	R Medicine (General)
Online Access:	http://eprints.usm.my/56181/1/DR%20HUSNAIDA%20BINTI%20ABDUL%20MANAN%40SULONG%20-%20e.pdf
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spelling	my-usm-ep.561812023-02-14T08:48:36Z Evaluation of the wound healing in full thickness skin autograft with different doses of topical stichopus spj extract in sprague dawley rats 2010 Sulong, Husnaida Abdul Manan R Medicine (General) Gamat refers to a collection of sea cucumbers most commonly consumed as traditional remedies by Malaysians. With its known healing properties to date, the effect of topical gamat extract could be beneficial in the healing process of the skin grafts and the effective dose could be determined. Objectives: The aim of this study was to investigate the effect of topical gamat from the species Stichopus spl at different concentrations (5 %, 10 % and 20 % w/w) on the healing process of skin auto grafts in female Sprague Dawley rats using macroscopic and microscopic parameters respectively. Method: This study was a randomized control trial with parallel design. Animals were randomly divided into control, low dose treated (5 %), medium dose treated (10 %) and high dose treated (20 %) groups. Dorsal full thickness skin sheets (2cm x 2cm) were harvested and were preserved between 6° to go Celsius for 5 days. 0.5 ml of topical substances (control and gamat extracts) was uniformly applied onto the graft wound beds at Day zero and Day five post-harvesting. Skin grafts were autotransplanted at Day five and fixed with 5-0 silk interrupted sutures. Seven days post graft transplantation, the grafts were assessed macroscopically and microscopically. Results: The animal mortality rate observed in this study is 27.6 %. Macroscopic assessment showed that there were no significant difference, in term of graft adherence, graft color and graft pliability between all gamat-treated groups and the controls. Semiquantitative microscopic assessment revealed that there were no significant difference in the infiltration of inflammatory cells (neutrophils, macrophages) and proliferation of fibroblasts and new blood vessels of the grafts between gamat-treated animals and the controls. However, there was a significant difference in term of graft plane separation between the controls and the medium dose treated group (10 %) (P<O.OOS). In all groups, the body weight showed significantly difference with declining pattern from Day zero to Day fivr post-harvesting (P<O.OS). There were no significant differences within groups seen from Day six to Day twelve. Cmrclusion: Application of Stich opus sp 1 extract (1 0 % w/w) seemed to hasten the acceptance of the skin graft by the wound bed, as demonstrated by the reduction of the plane separation following the application of the extract. Therefore, Stichopus spl extract does have an effect on the healing process of skin auto grafts in Sprague Dawley rats. 2010 Thesis http://eprints.usm.my/56181/ http://eprints.usm.my/56181/1/DR%20HUSNAIDA%20BINTI%20ABDUL%20MANAN%40SULONG%20-%20e.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian sains perubatan
institution	Universiti Sains Malaysia
collection	USM Institutional Repository
language	English
topic	R Medicine (General)
spellingShingle	R Medicine (General) Sulong, Husnaida Abdul Manan Evaluation of the wound healing in full thickness skin autograft with different doses of topical stichopus spj extract in sprague dawley rats
description	Gamat refers to a collection of sea cucumbers most commonly consumed as traditional remedies by Malaysians. With its known healing properties to date, the effect of topical gamat extract could be beneficial in the healing process of the skin grafts and the effective dose could be determined. Objectives: The aim of this study was to investigate the effect of topical gamat from the species Stichopus spl at different concentrations (5 %, 10 % and 20 % w/w) on the healing process of skin auto grafts in female Sprague Dawley rats using macroscopic and microscopic parameters respectively. Method: This study was a randomized control trial with parallel design. Animals were randomly divided into control, low dose treated (5 %), medium dose treated (10 %) and high dose treated (20 %) groups. Dorsal full thickness skin sheets (2cm x 2cm) were harvested and were preserved between 6° to go Celsius for 5 days. 0.5 ml of topical substances (control and gamat extracts) was uniformly applied onto the graft wound beds at Day zero and Day five post-harvesting. Skin grafts were autotransplanted at Day five and fixed with 5-0 silk interrupted sutures. Seven days post graft transplantation, the grafts were assessed macroscopically and microscopically. Results: The animal mortality rate observed in this study is 27.6 %. Macroscopic assessment showed that there were no significant difference, in term of graft adherence, graft color and graft pliability between all gamat-treated groups and the controls. Semiquantitative microscopic assessment revealed that there were no significant difference in the infiltration of inflammatory cells (neutrophils, macrophages) and proliferation of fibroblasts and new blood vessels of the grafts between gamat-treated animals and the controls. However, there was a significant difference in term of graft plane separation between the controls and the medium dose treated group (10 %) (P<O.OOS). In all groups, the body weight showed significantly difference with declining pattern from Day zero to Day fivr post-harvesting (P<O.OS). There were no significant differences within groups seen from Day six to Day twelve. Cmrclusion: Application of Stich opus sp 1 extract (1 0 % w/w) seemed to hasten the acceptance of the skin graft by the wound bed, as demonstrated by the reduction of the plane separation following the application of the extract. Therefore, Stichopus spl extract does have an effect on the healing process of skin auto grafts in Sprague Dawley rats.
format	Thesis
qualification_level	Master's degree
author	Sulong, Husnaida Abdul Manan
author_facet	Sulong, Husnaida Abdul Manan
author_sort	Sulong, Husnaida Abdul Manan
title	Evaluation of the wound healing in full thickness skin autograft with different doses of topical stichopus spj extract in sprague dawley rats
title_short	Evaluation of the wound healing in full thickness skin autograft with different doses of topical stichopus spj extract in sprague dawley rats
title_full	Evaluation of the wound healing in full thickness skin autograft with different doses of topical stichopus spj extract in sprague dawley rats
title_fullStr	Evaluation of the wound healing in full thickness skin autograft with different doses of topical stichopus spj extract in sprague dawley rats
title_full_unstemmed	Evaluation of the wound healing in full thickness skin autograft with different doses of topical stichopus spj extract in sprague dawley rats
title_sort	evaluation of the wound healing in full thickness skin autograft with different doses of topical stichopus spj extract in sprague dawley rats
granting_institution	Universiti Sains Malaysia
granting_department	Pusat Pengajian sains perubatan
publishDate	2010
url	http://eprints.usm.my/56181/1/DR%20HUSNAIDA%20BINTI%20ABDUL%20MANAN%40SULONG%20-%20e.pdf
_version_	1776101145837568000

Evaluation of the wound healing in full thickness skin autograft with different doses of topical stichopus spj extract in sprague dawley rats

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