Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam

Asparagus (Asparagus officinalis) is becoming more important in South East Asia (SEA) and quickly becoming a preferred vegetable. All varieties planted throughout SEA have been and now are still facing the most destructive disease i.e. asparagus crown and root rot. The main aim of the thesis was...

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Main Author: Al-Amodi, Mohamed Othman Saeed
Format: Thesis
Language:English
Published: 2006
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Online Access:http://eprints.usm.my/56227/1/00001648347%20MOSA-A.pdf
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spelling my-usm-ep.562272023-01-05T07:37:28Z Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam 2006-04 Al-Amodi, Mohamed Othman Saeed QH1-278.5 Natural history (General) Asparagus (Asparagus officinalis) is becoming more important in South East Asia (SEA) and quickly becoming a preferred vegetable. All varieties planted throughout SEA have been and now are still facing the most destructive disease i.e. asparagus crown and root rot. The main aim of the thesis was to collect and isolate Fusarium spp. from asparagus plants showing crown and root rot symptoms and their soils in Malaysia and Brunei Darussalam. The other objectives were to evaluate pathogenicity and genetic diversity within the Fusarium spp. and their variability using peR-based RAPD. A total of 110 isolates comprising five species of Fusarium have been isolated from six sampling areas in Malaysia and one in Brunei Darussalam. The five species identified were F. proliferatum, F. oxysporum, F. solani, F. semitectum and F. /ongipes, based on morphological characteristics. F. proliteretum and F. oxysporum represented the highest percentage (830/0). Pathogenicity tests of F. proiiteretum and F. oxysporum isolates by inoculating healthy asparagus seedlings var. UC157 in the greenhouse confirmed that all isolates tested were pathogenic. The typical symptoms were initially observed as yellowing of leaves and branches. Infected plants were stunted with reddishbrown discoloration and shrivelled roots. Sliced crowns and stems clearly showed reddish-brown discolorations of the infected tissues. Heavily infected plants collapsed and died. 2006-04 Thesis http://eprints.usm.my/56227/ http://eprints.usm.my/56227/1/00001648347%20MOSA-A.pdf application/pdf en public phd doctoral Perpustakaan Hamzah Sendut Pusat Pengajian Sains Kajihayat
institution Universiti Sains Malaysia
collection USM Institutional Repository
language English
topic QH1-278.5 Natural history (General)
spellingShingle QH1-278.5 Natural history (General)
Al-Amodi, Mohamed Othman Saeed
Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam
description Asparagus (Asparagus officinalis) is becoming more important in South East Asia (SEA) and quickly becoming a preferred vegetable. All varieties planted throughout SEA have been and now are still facing the most destructive disease i.e. asparagus crown and root rot. The main aim of the thesis was to collect and isolate Fusarium spp. from asparagus plants showing crown and root rot symptoms and their soils in Malaysia and Brunei Darussalam. The other objectives were to evaluate pathogenicity and genetic diversity within the Fusarium spp. and their variability using peR-based RAPD. A total of 110 isolates comprising five species of Fusarium have been isolated from six sampling areas in Malaysia and one in Brunei Darussalam. The five species identified were F. proliferatum, F. oxysporum, F. solani, F. semitectum and F. /ongipes, based on morphological characteristics. F. proliteretum and F. oxysporum represented the highest percentage (830/0). Pathogenicity tests of F. proiiteretum and F. oxysporum isolates by inoculating healthy asparagus seedlings var. UC157 in the greenhouse confirmed that all isolates tested were pathogenic. The typical symptoms were initially observed as yellowing of leaves and branches. Infected plants were stunted with reddishbrown discoloration and shrivelled roots. Sliced crowns and stems clearly showed reddish-brown discolorations of the infected tissues. Heavily infected plants collapsed and died.
format Thesis
qualification_name Doctor of Philosophy (PhD.)
qualification_level Doctorate
author Al-Amodi, Mohamed Othman Saeed
author_facet Al-Amodi, Mohamed Othman Saeed
author_sort Al-Amodi, Mohamed Othman Saeed
title Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam
title_short Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam
title_full Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam
title_fullStr Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam
title_full_unstemmed Morphological, Pathogenic, Genetic And Molecular Variabilities Of Fusarium Spp., The Pathogens Of Asparagus Crown And Root Rot In Malaysia And Brunei Darussalam
title_sort morphological, pathogenic, genetic and molecular variabilities of fusarium spp., the pathogens of asparagus crown and root rot in malaysia and brunei darussalam
granting_institution Perpustakaan Hamzah Sendut
granting_department Pusat Pengajian Sains Kajihayat
publishDate 2006
url http://eprints.usm.my/56227/1/00001648347%20MOSA-A.pdf
_version_ 1776101153730199552