Immunomodulatory and cell cycle regulatory effects of clinacanthus nutans
This research comprises analyses of proliferative activity, nitric oxide, cytokine and phagocytosis assays to evaluate its immunomodulatory properties as well as gene expression analysis of cell cycle regulatory genes. Murine macrophage cell line (J774A.1) was treated with various concentrations...
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my-usm-ep.566232023-02-28T08:57:35Z Immunomodulatory and cell cycle regulatory effects of clinacanthus nutans 2018-08 Ghazali, Wan Afiqah Syahirah Wan @ Wan Azmi QH573-671 Cytology R Medicine This research comprises analyses of proliferative activity, nitric oxide, cytokine and phagocytosis assays to evaluate its immunomodulatory properties as well as gene expression analysis of cell cycle regulatory genes. Murine macrophage cell line (J774A.1) was treated with various concentrations (250, 125, 62.5, 31.25 and 15.625 μg/ml) of aqueous and ethanol extracts of the leaves of C. nutans. MTS assay showed enhanced cell proliferation with an EC50 range between 4.051 to 100.6 μg/ml. Cytokine assay revealed that C. nutans possesses anti-inflammatory properties and increases the nitric oxide production in macrophages with a mean of production (41-130 μmol/L) that could modulate the inflammatory response. Percentage of latex beads IgG-FITC engulfment ranged between 14.9 and 55.1%. Phagocytic activity showed increase in percentage of latex beads IgG-FITC engulfment as the concentration of the extract increased. Human gingival fibroblast cell line (HGF-1) was treated with ethanol and aqueous extracts of Clinacanthus nutans (C. nutans) leaves for 1, 3 and 7 days. Gene expression analyses showed that BCL-2 was down-regulated in ethanol extract on day 1 and 7 indicating an anti-apoptotic effect and an up-regulation on day 3 indicating its apoptotic property. Down-regulation of BAX in aqueous extract on day 1 indicated the apoptotic nature of C. nutans and up-regulation of BAX in both the extracts on all days showed its pro-apoptotic properties. Down-regulation of C-MYC in aqueous extract on day 1 could result in decrease in the number of cells due to arrest in growth. Up-regulation of C-MYC in both the extracts at all days could inhibit signals for death and survival. Up-regulation of CDK1 in the ethanol extract on day 7 and aqueous extract on day 3 and 7 showed that C. nutans could regulate cell cycle progression and promote mitosis. There was down-regulation of SUMO-1 in ethanol extract on day 1, 3 and 7 and on day 1 and 3 in aqueous extract which results in the failure of SUMO-1 to conjugate to cellular proteins. There was up-regulation of SUMO-1 in aqueous extract on day 1, 3 and 7 and on day 1 and 3 in ethanol extract. The results of this study support the role of C. nutans extracts in regulating cellular processes such as apoptosis, protein stability, transcription and nuclear transport by modulating the gene expression of BCL-2, BAX, C-MYC, CDK1 and SUMO-1 thereby regulating cell cycle activity. 2018-08 Thesis http://eprints.usm.my/56623/ http://eprints.usm.my/56623/1/Dr.%20Wan%20Afiqah%20Syahirah%20Wan%20Ghazali-24%20pages.pdf application/pdf en public masters Universiti Sains Malaysia Pusat Pengajian Sains Perubatan |
| institution |
Universiti Sains Malaysia |
| collection |
USM Institutional Repository |
| language |
English |
| topic |
QH573-671 Cytology R Medicine |
| spellingShingle |
QH573-671 Cytology R Medicine Ghazali, Wan Afiqah Syahirah Wan @ Wan Azmi Immunomodulatory and cell cycle regulatory effects of clinacanthus nutans |
| description |
This research comprises analyses of proliferative activity, nitric oxide, cytokine
and phagocytosis assays to evaluate its immunomodulatory properties as well as gene
expression analysis of cell cycle regulatory genes. Murine macrophage cell line (J774A.1)
was treated with various concentrations (250, 125, 62.5, 31.25 and 15.625 μg/ml) of
aqueous and ethanol extracts of the leaves of C. nutans. MTS assay showed enhanced cell
proliferation with an EC50 range between 4.051 to 100.6 μg/ml. Cytokine assay revealed
that C. nutans possesses anti-inflammatory properties and increases the nitric oxide
production in macrophages with a mean of production (41-130 μmol/L) that could
modulate the inflammatory response. Percentage of latex beads IgG-FITC engulfment
ranged between 14.9 and 55.1%. Phagocytic activity showed increase in percentage of
latex beads IgG-FITC engulfment as the concentration of the extract increased. Human
gingival fibroblast cell line (HGF-1) was treated with ethanol and aqueous extracts of
Clinacanthus nutans (C. nutans) leaves for 1, 3 and 7 days. Gene expression analyses
showed that BCL-2 was down-regulated in ethanol extract on day 1 and 7 indicating an
anti-apoptotic effect and an up-regulation on day 3 indicating its apoptotic property.
Down-regulation of BAX in aqueous extract on day 1 indicated the apoptotic nature of C.
nutans and up-regulation of BAX in both the extracts on all days showed its pro-apoptotic
properties. Down-regulation of C-MYC in aqueous extract on day 1 could result in
decrease in the number of cells due to arrest in growth. Up-regulation of C-MYC in both
the extracts at all days could inhibit signals for death and survival. Up-regulation of CDK1 in the ethanol extract on day 7 and aqueous extract on day 3 and 7 showed that C. nutans
could regulate cell cycle progression and promote mitosis. There was down-regulation of
SUMO-1 in ethanol extract on day 1, 3 and 7 and on day 1 and 3 in aqueous extract which
results in the failure of SUMO-1 to conjugate to cellular proteins. There was up-regulation
of SUMO-1 in aqueous extract on day 1, 3 and 7 and on day 1 and 3 in ethanol extract.
The results of this study support the role of C. nutans extracts in regulating cellular
processes such as apoptosis, protein stability, transcription and nuclear transport by
modulating the gene expression of BCL-2, BAX, C-MYC, CDK1 and SUMO-1 thereby
regulating cell cycle activity. |
| format |
Thesis |
| qualification_level |
Master's degree |
| author |
Ghazali, Wan Afiqah Syahirah Wan @ Wan Azmi |
| author_facet |
Ghazali, Wan Afiqah Syahirah Wan @ Wan Azmi |
| author_sort |
Ghazali, Wan Afiqah Syahirah Wan @ Wan Azmi |
| title |
Immunomodulatory and cell cycle regulatory effects of clinacanthus nutans |
| title_short |
Immunomodulatory and cell cycle regulatory effects of clinacanthus nutans |
| title_full |
Immunomodulatory and cell cycle regulatory effects of clinacanthus nutans |
| title_fullStr |
Immunomodulatory and cell cycle regulatory effects of clinacanthus nutans |
| title_full_unstemmed |
Immunomodulatory and cell cycle regulatory effects of clinacanthus nutans |
| title_sort |
immunomodulatory and cell cycle regulatory effects of clinacanthus nutans |
| granting_institution |
Universiti Sains Malaysia |
| granting_department |
Pusat Pengajian Sains Perubatan |
| publishDate |
2018 |
| url |
http://eprints.usm.my/56623/1/Dr.%20Wan%20Afiqah%20Syahirah%20Wan%20Ghazali-24%20pages.pdf |
| _version_ |
1776101166863613952 |