Interaction Between Hydrogen Sulphide (H2s) And Nitric Oxide (No) In Left Ventricular Hypertrophy And Their Effect On Responsiveness Of Alpha I-adrenergic Receptors Subtypes In The Rat Kidney
The present study investigated the effect of left ventricular hypertrophy (LVH) on the responsiveness of (II-adrenergic receptor subtypes to adrenergic stimuli in the rat. The role of hydrogen sulphide (H2S) and nitric oxide (NO) systems and their interaction in the progression of LVH was studied...
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| Format: | Thesis |
| Language: | English |
| Published: |
2016
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| Subjects: | |
| Online Access: | http://eprints.usm.my/57701/1/00001808094%20Ashfaq%20Ahmad24.pdf |
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| Summary: | The present study investigated the effect of left ventricular hypertrophy
(LVH) on the responsiveness of (II-adrenergic receptor subtypes to adrenergic
stimuli in the rat. The role of hydrogen sulphide (H2S) and nitric oxide (NO) systems
and their interaction in the progression of LVH was studied by examining the effect
of altered expression of cystathione y lyase (CSE mRNA) and endothelial nitric
oxide synthase (eNOS mRN A) in the heart during LVH. Cardiovascular parameters
such as cardiac geometry, oxidative stress, arterial stiffness and vascular
responsiveness to vasoactive stimuli were studied. In addition, this study examined
renal excretory functions, haemodynamics and histopathological changes after
exogenous administration of NaHS, an H2S donor, L-arginine, an NO donor and a
combination of NaHS and L-arginine. Wistar-Kyoto (WKY) rats were divided into
two major groups of Control and LVH. These groups were then subdivided into 8
groups based on treatment. NaHS (56JlM/kg I.P. for 5 weeks) or L-arginine (1.25g1L
for 5 weeks in drinking water) treated control or LVH groups. LVH was induced
using isoprenaline (Smg/kg, S.c. every 72 hours for 2 weeks) and caffeine (62mglL
in drinking water for 2 weeks). The uj-adrenergic receptors subtypes was studied by
examining the responsiveness to noradrenaline (NA), phenylephrine (PE) and
methoxamine (ME) in the presence of a background intrarenal infusion of selective
uj-adrenergic receptors blockers (5-methylurapidil (5-MeU), chloroethy1clonidine
(CEC) and BMY 7378). Real-time quantitative peR data (normalized to |
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